| 129 | 4 | 43 |
| 下载次数 | 被引频次 | 阅读次数 |
为探讨陕西省桑园凹缘菱纹叶蝉种群间的遗传多样性、分子变异和遗传分化程度,本研究从陕西省7个主要蚕桑产区各选出一个重点县(区),采集凹缘菱纹叶蝉标本,PCR扩增得到其线粒体COⅠ基因核心序列。研究共得到了131条COⅠ基因序列,发现了229个变异位点,15个单倍型。总核苷酸多样性指数为0.065 8,各种群内核苷酸多样性0.010 9～0.120 0,总种群的单倍型为0.807 0,各种群内单倍型的多样性为0.709 1～0.857 1。总群体和各种群的Tajima's D检验结果均不显著,说明陕西桑园凹缘菱纹叶蝉的进化符合中性模型,种群数量较为稳定。分子变异方差分析结果表明,桑园凹缘菱纹叶蝉的种群的固定系数为0.124 37,种群内方差变异组分占总变异的87.56%,遗传分化主要来自于种群内部。这为进一步明晰其种群进化规律提供了参考。
Abstract：For the research of the genetic diversity, molecular variation and genetic differentiation of Hishimones sellatus Uhler in Shaanxi Province, samples were collected from the seven primary sericulture producing counties(districts) in Shaanxi province respectively, and the core sequence of COⅠ gene were amplified by PCR amplication.A total of 131 COⅠ sequences were obtained, and 229 mutation sites and 15 haplotypes were found. The total nucleotide diversity index was 0.065 8, ranging from 0.010 9 to 0.120 0 in each population. The haplotype diversity of the total population was 0.807 0, ranging from 0.709 1 to 0.857 1 in each population. The Tajima's D test for total population and each single population were not significant, which indicated that the evolution of Hishimonus sellatus Uhler in Shaanxi remained on neutral model and the population is relatively stable. Analysis of molecular variance showed that the FST of the the population was 0.124 37 and the variance components within population accounted for 87.56% of the total variation, and the genetic differentiation mainly occurred within the population. These results provide a reference for clear its population evolution.
Bandelt H.J.,Forster P.,and Rohl A.,1999,Median-joining networks for inferring intraspecific phylogenies,Mol.Biol.Evol.,16(1):37-48
Blouin M.S.,Yowell C.A.,Courtney C.H.,and Dame J.B.,1998,Substitution bias,rapid saturation,and the use of mt DNA for nematode systematics,Mol.Biol.Evol.,15(12):1719-1727
Drummond C.S.,Xue H.J.,Yoder J.B.,and Pellmyr O.,2010,Host-associated divergence and incipient speciation in the yucca moth Prodoxus coloradensis(Lepidoptera:Prodoxidae)on three species of host plants,Heredity(Edinb),105(2):183-196
Excoffier L.,and Lischer H.E.,2010,Arlequin suite ver 3.5:a new series of programs to perform population genetics analyses under Linux and Windows,Mol.Ecol.Resour.,10(3):564-567
Folmer O.,Black M.,Hoeh W.,Lutz R.,and Vrijenhoek R.,1994,DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates,Mol.Mar.Biol.Biotechnol.,3(5):294-299
Fletcher M.,Locker H.,Mitchell A.,and Gopurenko D.,2017,Arevision of the genus orosius distant(Hemiptera:Cicadellidae)based on male genitalia and DNA barcoding,Austral Entomology,56(2):198-217
Hebert P.D.,Cywinska A.,Ball S.L.,and de Waard J.R.,2003,Biological identifications through DNA barcodes,Proc.Biol.Sci.,270(1512):313-321
Kimura M.,1980,A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences,J.Mol.Evol.,16(2):111-120
Kumar S.,Stecher G.,and Tamura K.,2016,MEGA7:Molecular evolutionary genetics analysis version 7.0 for bigger datasets,Mol.Biol.Evol.,33(7):1870-1874
Librado P.,and Rozas J.,2009,DnaSP v5:an software for comprehensive analysis of DNA polymorphism data,Bioinformatics,25(11):1451-1452
Pan C.Y.,Hu J.,Zhang X.,and Huang Y.,2006,The DNA barcoding application of mt DNA COⅠgenes in seven species of catantopidae(Orthoptera),Kunchong Fenlei Xuebao(Entomotaxonomia),28(2):103-110(潘程莹,胡婧,张霞,黄原,2006,斑腿蝗科Catantopidae七种蝗虫线粒体COⅠ基因的DNA条形码研究,昆虫分类学报,28(2):103-110)
Simon C.,Buckley T.R.,Frati F.,Stewart J.B.,and Beckenbach A.T.,2006,Incorporating molecular evolution into phylogenetic analysis,and a new compilation of conserved polymerase chain reaction primers for animal mitochondrial DNA,Annu.Rev.Ecol.Evol.S.,37(37):545-579
Tajima F.,1989,Statistical method for testing the neutral mutation hypothesis by DNA polymorphism,Genetics,123(3):585-595
Thompson J.D.,Gibson T.J.,Plewniak F.,Jeanmougin F.,and Higgins D.G.,1997,The CLUSTAL_X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools,Nucleic.Acids.Res.,25(24):4876-4882
Wang C.Y.,and Mam timin S.,2017,Genetic differentiation of nrDNA ITS sequence of Bryum argenteum Hedw(Bryaceae)populations in Xinjiang uygur autonomous region,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(4):1426-1435(汪琛颖,买买提明·苏来曼,2017,新疆银叶真藓居群nrDNAITS序列的遗传分化,分子植物育种,15(4):1426-1435)
Wei S.J.,and Chen X.X.,2011,Progress in research on the comparative mitogenomics of insects,Yingyong Kunchong Xuebao(Chinese Journal of Applied Entomology),48(6):1573-1585(魏书军,陈学新,2011,昆虫比较线粒体基因组学研究进展,应用昆虫学报,48(6):1573-1585)
Yin M.H.,He L.S.,Zhu Q.Z.,Xia H.Y.,and Hong S.R.,2017,Identification of early pear main cultivars in shangrao and genetic stability SSR analysis of their regenerated plants,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(11):3120-3129(尹明华,何林森,朱奇志,夏华炎,洪森荣,2016,上饶早梨品种鉴定及再生苗遗传稳定性SSR检测,分子植物育种,14(11):3120-3129)
Zhang Y.B.,Yang N.W.,Zhang G.F.,Wang J.J.,and Wan F.H.,2015,Identification of naturally occurring Eretmocerus parasitoids by COⅠsequence analysis and crossing test,Huanjing Kunchong Xuebao(Journal of Environmental Entomology),37(2):281-292(张毅波,杨念婉,张桂芬,王进军,万方浩,2015,利用COⅠ基因序列和杂交实验分析鉴定桨角蚜小蜂属的本地种群,环境昆虫学报,37(2):281-292)
Zhou N.N.,Wang M.X.,Cui L.,Pan C.,Zhang X.T.,and Han B.Y.,Genetic variation of Empoasca vitis(GOthe)(Hemiptera:Cicadellidae)among different geographical populations based on mt DNA COⅠcomplete sequence,Shengtai Xuebao(Acta Ecologica Sinica),34(23):6879-6889(周宁宁,王梦馨,崔林,潘铖,张新亭,韩宝瑜,2014,基于COⅠ基因全长序列的假眼小绿叶蝉地理种群遗传分化研究,生态学报,34(23):6879-6889)
基本信息:
DOI：10.13417/j.gab.038.003472
中图分类号:S888.72
引用信息:
[1]杨金宏,孔卫青.基于COⅠ序列的陕西凹缘菱纹叶蝉种群关系与遗传多样性[J].基因组学与应用生物学,2019,38(08):3472-3478.DOI:10.13417/j.gab.038.003472.
基金信息:
陕西省教育厅重点实验室科研计划项目(No.14JS003)资助
2019-08-25
2019-08-25