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以落叶松种子园中21个长白落叶松无性系亲本为试验材料,对其结实和发芽性状进行研究,分析其遗传变异状况,以期筛选结实及发芽均表现优良的无性系。方差分析结果表明各无性系结实和发芽性状间存在极显著差异,其中无性系L52和L67平均发芽特性表现较好。各性状表型变异系数变化范围为13.34%～69.47%,遗传力变化范围为0.7757～0.9463,高变异系数、高遗传力有利于优良无性系评价选择。相关分析结果表明各性状间相关系数变化范围为0.054～0.889,其中种子发芽率、发芽势、发芽指数与千粒重、种子长度、种子宽度存在显著或极显著正相关,表明种子发芽特性与种子表型间存在较为密切的联系。最终以发芽率、发芽势和发芽指数为指标进行聚类分析,将各无性系种子聚为两类,无性系L17、L40、L49、L52、L55、L67、L71、L88和L99种子发芽性状较优良,可作为高发芽率优良亲本进行利用,此研究可为长白落叶松种子园亲本选择提供依据。
Abstract：With 21 Larix olgens is cl ones a s experimental materials, the fruit and seed germination traits were investigated and genetic variation analysis was conducted, which was hoped to select the clones with excellent seed germination and fruit characteristics. ANOVA analysis of fruit and seed germination traits showed that there was extremely significant difference among all clones of which L52 and L67 showed the better performance of seed germination. The phenotypic coefficients of all traits ranged from 13.34% to 69.47% and the heritability ranged from 0.7757 to 0.9463, the higher phenotypic coefficient and repeatability were conductive to excellent clone selection. Correlation analysis showed that the correlation coefficients among all traits were different, which varied from 0.054 to 0.889, and there was extremely significant or significant difference between germination rate,germination potential, germination index and thousand seed weight, seed length, and seed width, which indicated that there was close relationship between phenotype and seed germination. Using the germination rate, germination potential and germination index as cluster index to carry out analysis, 21 clones were divided into two categories.The clone L17, L40, L49, L52, L55, L67, L71, L88 and L99 possessed better performance of seed germination characteristics, which could be utilized as the excellent parents with high germination. The study might provide thetheoretical basis for excellent parent selection of Larix olgensis.
Burdon R.D.,1977,Genetic correlation as concept for studying genotype-environment interactions in forest breeding,Silvae Genet.,26:168-175
Chen N.L.,Wang P.,Wu P.F.,Jia Y.Y.,and Ma X.Q.,2015,Differences in seed biological characteristics of differen half-sib Chinese fir,Senlin Yu Huanjing Xuebao(Journal of Forest and Environment),35(3):230-235(陈奶莲,汪攀,吴鹏飞,贾亚运,马祥庆,2015,不同杉木半同胞家系种子生物学特性的差异,森林与环境学报,35(3):230-235)
Chen H.L.,Li Q.M.,Ma F.Y.,Liu Y.,and Zhang J.F.,2010Physiological and biochemical characteristics of Larix olgensis and L.gmelini seeds under ultra-dry treatment Zhongguo Shengtai Nongye Xuebao(Chinese Journal of E-co-Agriculture),18(3):570-575(陈怀梁,李庆梅,马风云刘艳,张俊风,2010,超干处理两种落叶松种子的生理生化特征研究,中国生态农业学报,18(3):570-575)
Guan C.Y.,Wang F.S.,Li S.S.,and Li J.,2014,Seed characteristics&sowing and seedling-raising technology for Larix laricina,Fanghulin Keji(Protection Forest Science and Technology),(3):11-13(贯春雨,王福森,李树森,李晶,2014阿拉斯加落叶松种子特性与播种育苗技术的研究,防护林科技,(3):11-13)
Hansen J.K.,and Roulund H.,1997,Genetic parameters for spira grain,stem form,pilodyn and growth in 13 years old clones of Sitka Spruce(Picea sitchensis(Bong.)Carr.),Silvae Genet.,46(7):107-112
Li Q.M.,Fu Z.J.,and Zhang H.Y.,2007,Effects of chitosam seed germination of Larix olgensis and Platycladus orientalis Linye Kexue Yanjiu(Forest Research),20(4):524-527(李庆梅,付增娟,张洪燕,2007,壳聚糖对长白落叶松和侧柏种子萌发的影响,林业科学研究,20(4):524-527)
Pliura A.,Zhang S.Y.,Mackay J.,and Bousquet J.,2007,Genotypic variation in wood density and growth traits of poplar hybrids at four clonal trials,Forest Ecology and Management,238(1):92-106
Qin X.J.,and Ding G.J.,2012,Analysis on the seed characters between difference geographical of Pinus massoniana and seedling stage growth,Zhongzi(Seed),31(01):14-17(秦晓佳,丁贵杰,2012,不同地理种源马尾松种子性状及芽苗生长分析,种子,31(01):14-17)
Sun Y.F.,and Lu Y.S.,2007,A grid based subspace clustering algorithm for high dimensional data streams,Jisuanji Kexue(Computer Science),34(4):199-203(孙玉芬,卢炎生,2007,一种基于网格方法的高维数据流子空间聚类算法,计算机科学,34(4):199-203)
Wang Y.L.,and Li Y.,2008,Study on phenotypic diversity of cone and seed in natural populations of Picea crassifolia in Qilian mountain,China,Zhiwu Shengtaixue Bao(Journal of Plant Ecology),32(2):355-362(王娅丽,李毅,2008,祁连山青海云杉天然群体的结实性状表型多样性,植物生态学报,32(2):355-362)
Wang Z.B.,Ji M.,Zhou Y.J.,Ren J.M.,and Zhang Y.X.,2015,Study on germination property of Larix gmelinii seed in different provenances,Linye Keji(Forestry Science and Technology),40(1):14-17(王志波,季蒙,周玉杰,任建民,张玉霞,2015,兴安落叶松不同种源发芽特性研究,林业科技,40(1):14-17)
Wang Z.B.,Ji M.,Ren J.M.,Hu Z.L.,Zhao D.W.,and Qi Z.X.,2012,Germination characteristics of seeds of different provenances of Larix principis-rupprechtii,Neimenggu Linye Keji(Journal of Inner Mongolia Forestry Science and Technology),38(4):11-15(王志波,季蒙,任建民,胡占林,赵德旺,齐振祥,2012,不同种源华北落叶松种子发芽特性研究,内蒙古林业科技,38(4):11-15)
Wei Z.G.,Gao Y.C.,Yang C.P.,Liu G.f.,Wang J.,and Liu G.J.,2009,Phenotypic traits and germination characters of Pinus sylvestris ssp.Kulendensis seeds from different provenances introduced from abroad,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),37(11):7-10(魏志刚,高玉池,杨传平,刘桂丰,王军,刘关君,2009,引种盐松不同种源种子表型性状和发芽特性,东北林业大学学报,37(11):7-10)
Wei Z.G.,Gao Y.C.,Yang C.P.,Liu G.F.,Wang J.,and Liu G.J.,2009,Seeds germination feature of different Pinus slvestrisl ssp kulendensis sukaczw provenances under Na Cl stress,Zhongzi(Seed),28(08):9-13(魏志刚,高玉池,杨传平,刘桂丰,王军,刘关君,2009,Na Cl胁迫下盐松不同种源种子的发芽特性,种子,28(08):9-13)
Xu H.W.,Liu Y.,Li Y.J.,Teng W.H.,Jiang J.,and Zhao X.Y.,2013,Comparative analysis on seed vitality of different families produced in Betula pendula Triploid seed orchard,Xinan Linye Daxue Xuebao(Journal of Southwest Forestry U-niversity),33(5):34-39(徐焕文,刘宇,李雅婧,滕文华,姜静,赵曦阳,2013,白桦三倍体制种园中各家系种子活力比较,西南林业大学学报,33(5):34-39)
Zhang J.G.,Duan A.G.,Zhang P.J.,Pei D.,Luo H.M.,and Shan J.Y.,2006,A study on seed characteristics of largeberry cultivars of sea buckthorn,Linye Kexue Yanjiu(Forest Research),19(6):700-705(张建国,段爱国,张俊佩,裴东,罗红梅,单金友,2006,不同品种大果沙棘种子特性研究,林业科学研究,19(6):700-705)
Zhao X.Y.,Wang J.H.,Zhang J.F.,Zhang S.G.,Zhang J.G.,and Ma J.W.2008,Study on phenotypic traits and germination characters of four taxons of catalpa genus seed,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&FUniversity),36(12):149-154(赵曦阳,王军辉,张金凤,张守攻,张建国,马建伟,2008,梓树属4个种种子表型性状和发芽特性的研究,西北农林科技大学学报(自然科学版),36(12):149-154)
Zheng L.C.,Tian G.X.,Jiang X.P.,and Peng H.F.,1999,An analysis of germination of Paulownia tomentosa seeds in different provenances and its cluster,Shengwu Shuxue Xuebao(Journal of Biomathematics),14(1):86-89(郑兰长,田国行,蒋小平,彭海凤,1999,不同种源毛泡桐种子发芽状况及其聚类分析,生物数学学报,14(1):86-89)
基本信息:
DOI：10.13417/j.gab.037.001334
中图分类号:S791.22
引用信息:
[1]张连生,赵国辉,孙晓阳,等.长白落叶松结实与发芽特性[J].基因组学与应用生物学,2018,37(03):1334-1342.DOI:10.13417/j.gab.037.001334.
基金信息:
“十三五”国家重点研发课题(No.2017YFD0600402)资助
2017-12-28
2017-12-28
2017-12-28