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为研究饲料中分别添加芽孢杆菌(Bacillus)和乳酸菌(Lactobacillus)2种益生菌对斑点叉尾鮰(Ictalurus punctatus)肠道微生物群落以及菌群代谢物的影响，本研究采用16S rRNA基因高通量技术和基于液相色谱的代谢组学技术分析斑点叉尾鮰肠道菌群组成以及代谢组学。选取体重为(50±3) g的斑点叉尾鮰360尾，随机分为3组，分别为对照组(DZ),芽孢杆菌组(YB)以及乳酸菌组(RS),每组3个平行，试验周期为11周。结果表明，饲料中添加芽孢杆菌和乳酸菌均可提高菌群Alpha多样性，在门水平上，厚壁杆菌门(Firmicutes)与拟杆菌门(Bacteroidetes)的比值线性降低，厚壁杆菌门丰度显著低于对照组的(P<0.05),而变形杆菌门(Proteobacteria)、放线菌门(Actinobacteria)的相对丰度显著高于对照组的(P<0.05);在属水平上，与对照组相比，伯克霍尔德菌属(Burkholderia-Caballeronia-Paraburkholderia)的相对丰度显著提高(P<0.05),而苏黎世杆菌属(Turicibacter)和罗姆布茨菌属(Romboutsia)的相对丰度显著下降(P<0.05)。不同分组之间的差异代谢物分析发现，与对照组相比，芽孢杆菌组和乳酸菌组的差异代谢物5-羟基吲哚乙酸相对含量极显著上调(P<0.01),黄嘌呤酸相对含量极显著下调(P<0.01)。综上，饲料中添加芽孢杆菌和乳酸菌可改善斑点叉尾鮰肠道微生物群落，增加群落多样性，提高伯克霍尔德菌属等有益菌的相对丰度，提高吲哚类化合物含量，降低黄嘌呤酸的含量，继而提高斑点叉尾鮰免疫力，维持肠道菌群稳态。
Abstract：In order to investigate the effects of two probiotics, Bacillus and Lactobacillus, respectively, on the intestinal microbial community and microbiota metabolites of channel catfish(Ictalurus punctatus), 16S rRNA high throughput sequencing and liquid chromatography-based metabolomics technology were used to analyze the intestinal microbiota composition and metabolomics of channel catfish. A total of 360 channel catfish with a body weight of(50±3) g were selected and randomly divided into 3 groups, and named as the control group, the Bacillus group and the Lactobacillus group. There were 3 parallels in each group, and the experiment lasted 11 weeks. The results showed that the addition of Bacillus and Lactobacillus to the feed could improve the Alpha diversity of the intestinal microbiota. In the phylum level, the ratio of Firmicutes to Bacteroides was linear decreased; the abundance of Firmicutes was significantly lower than that of the control group(P<0.05), and the relative abundance of Proteobacteria and Actinobacteria was significantly higher than that of the control group(P<0.05). While in the genus level, compared with the control group, the relative abundance of Burkholderia-Caballeronia-Paraburkholderia was significantly increased(P<0.05), while the relative abundance of Turicibacter and Romboutsia were significantly decreased(P<0.05). The metabolite analysis between different groups showed that compared with the control group, the relative content of 5-hydroxyindoleacetic acid in the Bacillus group and Lactobacillus group was very significantly increased(P<0.01), and the relative content of fulvic acid was very significantly lowered(P<0.01). In summary, the addition of Bacillus and Lactobacillus to feed could improve the intestinal microbial community of channel catfish, increase community diversity, increase the relative abundance of beneficial bacteria such as Burkholderia-Caballeronia-Paraburkholderia, increase the content of indole compounds, reduce the content of fulvic acid, and then improve the immunity of channel catfish and maintain the homeostasis of intestinal microbiota.
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基本信息:
DOI：10.13417/j.gab.042.001100
中图分类号:S965.128
引用信息:
[1]周丽颖,钟立强,张世勇,等.芽孢杆菌和乳酸菌对斑点叉尾鮰肠道菌群及代谢组学的影响[J].基因组学与应用生物学,2023,42(10):1100-1114.DOI:10.13417/j.gab.042.001100.
基金信息:
国家自然科学基金项目(32002378); 国家特色淡水鱼产业技术体系项目(CARS-46); 江苏现代农业(大宗鱼类)产业技术体系种质创新与苗种繁育创新团队项目[JATS(2022)413]共同资助
2023-06-14
2023-06-14
2023-06-14