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植物特有的NAC转录因子在植物的生长发育、胁迫应答和激素调节等方面具有重要功能。本研究从橡胶树胶乳cDNA中克隆了HbNAC33基因,生物信息学分析显示该基因的完整开放阅读框(ORF)为1 092 bp,编码363个氨基酸。HbNAC33蛋白的N-端第3~156位氨基酸之间含有一个典型的NAC结构域,酵母试验表明,HbNAC33具有转录激活活性,转录激活区在C-端,具备了NAC转录因子的基本特征。序列比对和系统进化分析表明HbNAC33蛋白属于NAC转录因子家族中的ANAC011亚族。实时荧光定量PCR分析表明,HbNAC33在叶中的表达量显著高于其他部位;割胶,乙烯利(ET)和茉莉酸(JA)处理均能诱导HbNAC33的表达上调。以上结果表明,HbNAC33可能参与植物的生长发育和胁迫应答过程。
Abstract：Plant-specific NAC transcription factors play crucial roles in plant growth and development, hormone regulations and stress response. In this study, the whole open reading frame(ORF) sequence of HbNAC33 was cloned from cDNA of Hevea brasiliensis Müll. Arg. latex. Bioinformatics analysis showed that HbNAC33 with an ORF of 1 092 bp, encoding a protein with 363 amino acids. It's N-terminal region had a conserved NAC domain from amino acid 3 to 156. The HbNAC33 protein possesses transcriptional activation activity and the transcription regulatory region was located in the C-terminal region in yeast assay system. Sequence alignment and phylogenetic analysis revealed that HbNAC33 was a member of a NAC transcription factor family belonging to ANAC011 subgroup. The Real-time PCR indicated that expression level of HbNAC33 in leaves was significantly higher than that in other parts of the rubber tree. The expression of HbNAC33 in latex was induced by tapping, ethephon(ET) and jasmonic acid(JA). The results suggested that HbNAC33 may be involved in response to stress and developmental processes in rubber tree.
Al Abdallat M.A.,Ayad J.Y.,Abu Elenein J.M.,Al Ajlouni Z.,and Harwood W.A.,2014,Overexpression of the transcription factor HvSNAC1 improves drought tolerance in barley(Hordeum vulgare L.),Mol.Breeding,33(2):401-414
Cenci A.,Guignon V.,Roux N.,and Rouard M.,2014,Genomican alysis of NAC transcription factors in banana(Musaacuminata) and definition of NAC orthologous groups formono cots and dicots,Plant Mol.Biol.,85(1-2):63-80
Chen X.,Wang Y.F.,Lv B.,Li J.,Luo L.Q.,Lu S.C.,Zhang X.,Ma H.,and Ming F.,2014,The NAC family transcription factor Os NAP confers abiotic stress response through the ABA pathway,Plant Cell Physiol.,55(3):604-619
Hao B.Z.,and Wu J.L.,2000,Laticifer differentiation in Hevea brasiensis:Induction by exogenous jasmonic acid and linolenic acid,Ann.Bot.,85(1):37-43
Jiang X.Q.,Zhang C.Q.,LüP.T.,Jiang G.M.,Liu X.W.,Dai F.W.,and Gao J.,2014,RhNAC3,a stress-associated NAC transcription factor,has a role in dehydration tolerance through regulating osmotic stress-related genes in rosepetals,Plant Biotechnol.J.,12(1):38-48
Kang G.J.,Zeng R.Z.,Nie Z.Y.,Dai L.J.,Duan C.F.,and Li Y.,2012,Cloning and bioinformatics analysis of a membrane-bound NAC transcription factor gene HbNTL1 from Hevea brasiliensis,Zhongguo Nongxue Tongbao(ChineseAgri cultural Science Bulletin),28(31):7-14(康桂娟,曾日中,聂智毅,代龙军,段翠芳,黎瑜,2012,巴西橡胶树膜结合NAC转录因子HbNTL1的克隆及生物信息学分析,中国农学通报,28(31):7-14)
Kang G.J.,Zeng R.Z.,Nie Z.Y.,Li Y.,Dai L.J.,and Duan C.F.,2012,Cloning and bioinformatics analysis of a NAC Transcription factor HbNAC1 from Hevea brasiliensis,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),28(34):1-11(康桂娟,曾日中,聂智毅,黎瑜,代龙军,段翠芳,2012,巴西橡胶树NAC转录因子HbNAC1基因的克隆及生物信息学分析,中国农学通报,28(34):1-11)
Kim H.J.,Hong S.H.,Kim Y.W.,Lee I.H.,Jun J.H.,Phee B.K.,Rupak T.,Jeong H.,Lee Y.,Hong B.S.,Nam H.G.,Woo H.R.,and Lim P.O.,2014,Gene regulatory cascade of senescence-associated NAC transcription factors activated by ETHYLENE-INSENSITIVE2-mediated leaf senescencesi gnalling in Arabidopsis,J.Exp.Bot.,65(14):4023-4036
Kim S.G.,and Park C.M.,2007,Membrane-mediated salt stress signaling in flowering time control,Plant Signal.Behave.,2(6):517-518
Li X.L.,Yang X.,Hu Y.X.,Yu X.D.,and Li Q.L.,2014,A novel NAC transcription factor from Suaeda liaotungensis K.enhanced transgenic Arabidopsis drought,salt,and cold stressto lerance,Plant Cell Rep.,33(5):767-778.
Li P.,Zhou H.,Shi X.L.,Yu B.,Zhou Y.,Chen S.L.,Wang Y.F.,Peng Y.,Meyer R.C.,Smeekens S.C.,and Teng S.,2014,The ABI4-induced Arabidopsis ANAC060 transcription factor attenuates ABA signaling and renders seedlings sugarin sensitive when present in the nucleus,PLoS genet.,10(3):e1004213
Liu G.Z.,Li X.L.,Jin S.X.,Liu X.Y.,Zhu L.F.,Nie Y.C.,and Zhang X.L.,2014,Overexpression of rice NAC geneSNAC1 improves drought and salt tolerance by enhancing root development and reducing transpiration rate in transgenic cotton,PloS One,9(1):e86895
Nuruzzaman M.,Manimekalai R.,Sharoni A.M.,Satoh K.,Kondoh H.,Ooka H.,and Kikuchi S.,2010,Genome-wide analysis of NAC transcription factor family in rice,Gene,465(1-2):30-44
Nuruzzaman M.,Sharoni A.M.,and Kikuchi S.,2013,Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants,Front.Microbiol.,4:248
Ooka H.,Satoh K.,Doi K.,Nagata T.,OtomoY.,Murakami K.,Matsubara K.,Osato N.,Kawai J.,Carninci P.,HayashizakiY.,Suzuki K.,Kojima K.,Takahara Y.,Yamamoto K.,and Kikuchi S.,2003,Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana,DNA Res.,10(6):239-247
Puranik S.,Sahu P.P.,Srivastava P.S.,and Prasad M.,2012,NAC proteins:Regulation and role in stress tolerance,Trends Plant Sci.,17(6):369-381
Qiu Q.,Zhu J.H.,and Zhang Z.L.,2012,Cloning and expressionan alysis of HbNAC1 in Hevea brasiliensis,Redai YaredaiZhiwu Xuebao(Journal of Tropical and Subtropical Botany),20(5):469-474(邱琼,朱家红,张治礼,2012,巴西橡胶树HbNAC1基因的克隆和表达分析,热带亚热带植物学报,20(5):469-474)
Quach T.N.,Tran L.S.,Valliyodan B.,Nguyen H.T.,Kumar R.,Neel akandan A.K.,Guttikonda S.K.,Sharp R.E.,andNguyen H.T.,2014,Functional analysis of water stress-responsive soybean GmNAC003 and GmNAC004 transcription factors in lateral root development in Arabidopsis,PloSOne,9(1):e84886
Rachmat A.,Nugroho S.,Sukma D.,and Aswidinnoor H.,2014,Overex pression of OsNAC6 transcription factor from Indonesia rice cultivar enhances drought and salt to lerance,Emir.J.Food Agric.,26(6):519-527
Shan W.,Kuang J.F.,Lu W.J.,and Chen J.Y.,2014,A bananafruit NAC transcription factor MaNAC1 is a direct target ofMaICE1 and involved in cold stress through interacting with MaCBF1,Plant Cell Environ.,37(9):2116-2127
Singh A.K.,Sharma V.,Pal A.K.,Acharya V.,and Ahuja P.S.,2013,Genome-wide organization and expression profilingof the NAC transcription factor family in potato(Solanumtu berosum L.),DNA Res.,20(4):403-423
Su H.Y.,Zhang S.Z.,Yin Y.L.,Zhu,D.Z.,and Han L.Y.,2014,Genome-wide analysis of NAM-ATAF1,2-CUC2 transcription factor family in Solanum lycopersicum,J.Plant Biochem.Biot.,255-259
Su H.Y.,Zhang S.Z.,Yuan X.W.,Chen C.T.,Wang X.F.,andHao Y.J.,2013,Genome-wide analysis and identification ofstress-responsive genes of the NAM-ATAF1,2-CUC2 transcription factor family in apple,Plant Physiol.Bioch.,71:11-21
Souer E.,van Houwelingen A.,Kloos D.,Mol J.,and Koes R.,1996,The no apical meristem gene of Petunia is required forpattern formation in embryos and flowers and is expressed atmeristem and primordia boundaries,Cell,85(2):159-170
Wang Z.Y.,and Dane F.,2013,NAC(NAM/ATAF/CUC)transcription factors in different stresses and their signalingpathway,Acta.Physiol.Plant.,35(5):1397-1408
Xu Q.F.,He Q.,Li S.,and Tian Z.D.,2014,Molecular characterization of StNAC2 in potato and its overexpression confersdrought and salt tolerance,Acta Physiol.Plant.,36(7):1841-1851
Yang X.,Hu Y.X.,Li X.L.Yu X.D.,and Li Q.L.,2014,Molecular characterization and function analysis of SlNAC2 inSuaeda liaotungensis K.,Gene.,543(2):190-197
Yu X.W.,Peng H.,Liu Y.M.,Zhang Y.,Shu Y.J.,Chen Q.J.,ShiS.B.,Ma L.,Ma H.,and Zhang H.,2014,CarNAC2,a novelNAC transcription factor in chickpea(Cicer arietinum L.),isassociated with drought-response and various developmen talprocesses in transgenic Arabidopsis,J.Plant Biol.,57(1):55-66
Zeng R.Z.,Duan C.F.,Li X.Y.,Tian W.M.,and Nie Z.Y.,2009,Vacuolar-type inorganic pyrophosphatase located on therubber particle in the latex is an essential enzyme in regulation of the rubber biosynthesis in Hevea brasiliensis,PlantSci.,176(5):602-607
Zhang J.J.,Chen J.,Yi Q.,Hu Y.F.,Liu H.M.,Liu Y.H.,andHuang Y.B.,2014,Novel role of ZmaNAC36 in co-expression of starch synthetic genes in maize endosperm,PlantMol.Biol.,84(3):359-369
Zhao Y.J.,Sun J.Y.,Xu P.,Zhang R.,and Li L.G.,2014,Wood-associated NAC transcription factor1B regulates cellwall thickening during fiber development in Populusspecies,Plant Physiol.,164(2):765-776
Zhu M.K.,Chen G.P.,Zhou S.,Tu Y.,Wang Y.,Dong T.T.,andHu Z.L.,2014,A new tomato NAC(NAM/ATAF1/2/CUC2)transcription factor,SlNAC4,functions as a positiveregulator of fruit ripening and carotenoid accumulation,Plant Cell Physiol.,55(1):119-135
基本信息:
DOI：10.13417/j.gab.033.000845
中图分类号:S794.1
引用信息:
[1]康桂娟,黎瑜,曾日中.巴西橡胶树HbNAC33基因的克隆和表达分析[J].基因组学与应用生物学,2014,33(04):845-852.DOI:10.13417/j.gab.033.000845.
基金信息:
国家自然科学基金项目(31201143);; 海南省自然科学基金项目(313061);; 中国热带农业科学院橡胶研究所基本科研业务费重点项目(1630022011024)共同资助
2014-08-28
2014-08-28