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本研究分析了不同人工饲料(S1, S2和S3)对马氏珠母贝(Pinctada fucata martensii)消化酶、免疫和生长相关基因表达的影响。共设置了6个实验组(EG1, EG2, EG3, EG4, EG5, EG6)和1个对照组(CG)。实验组EG1、EG2、EG3分别只投喂饲料S1、S2和S3,EG4投喂饲料S1+亚心形扁藻(Platymonas subcordiformis),EG5投喂饲料S2+亚心形扁藻,EG6投喂饲料S3+亚心形扁藻。对照组CG仅投喂亚心形扁藻。养殖60d,结果显示:(1) EG5、EG6和CG的蛋白酶活力显著的高于其他组(p<0.05);EG6和CG淀粉酶活性显著高于其他组(p<0.05)。(2) EG5、EG6和CG的FGF18、TβR I和GHITM m RNA的表达水平显著高于其他组(p<0.05)。(3) EG1的免疫酶(ALP, SOD, CAT和POD)活性最低,MDA含量最高;EG1、EG2和EG3中SOD mRNA的表达水平显著低于EG5, EG6和CG (p<0.05);EG1、EG2和EG3 GPx mRNA的表达水平显著低于EG5、EG6和CG (p<0.05);EG1、EG2和EG3 CAT mRNA的相对表达水平显著低于EG6和CG (p<0.05)。因此,饲料S2和S3与微藻混合投喂可以促进马氏珠母贝的消化吸收以及抗应激的能力,从而获得较好的生长性能和保持最佳的免疫机能。
Abstract：This study analyzed the effects of different artificial diets(S1, S2 and S3) on digestive enzymes, immunity and growth-related gene expression in Pinctada fucata martensii. A total of six experimental groups(EG1, EG2,EG3, EG4, EG5 and EG6) and one control group(CG) were set up. EG1, EG2 and EG3(experimental groups)were fed with S1, S2 and S3, respectively. EG4 was fed with S1 +Platymonas subcordiformis, EG5 was fed with S2+Platymonas subcordiformis, EG6 was fed with S3+Platymonas subcordiformis. CG(control group) was only fed with P. subcordiformis. After 60 days of breeding, the results showed that:(1) The protease activities of EG5, EG6 and CG were significantly higher than those of other groups(p<0.05); the activities of EG6 and CG amylase were significantly higher than other those of groups(p<0.05).(2) the expression levels of FGF18, TβR I and GHITM mRNA in EG5, EG6 and CG were significantly higher than those in other groups(p<0.05).(3) The activity of Immunoenzymes(ALP, SOD, CAT and POD) of EG1 was the lowest and the content of MDA was the highest;.The mRNA expression of SOD in EG1, EG2 and EG3 were significantly lower than those in EG5, EG6 and CG(p<0.05). The mRNA expression of GPx in EG1, EG2 and EG3 were significantly lower than those in EG6 and CG(p<0.05). And the relative expression levels of CAT mRNA in EG1, EG2 and EG3 were significantly lower than those in EG6 and CG(p< 0.05). Thus, the mixed feeding of S2 and S2 with microalgae could promote digestion and absorption of P. f. martensii and its ability to resist stress, so as to obtain better growth performance and maintain the best immune function.
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基本信息:
DOI：10.13417/j.gab.037.005251
中图分类号:S917.4
引用信息:
[1]廖永山,杨创业,王庆恒,等.不同饲料对马氏珠母贝(Pinctada fucata martensii)消化酶、免疫和生长相关基因表达的影响[J].基因组学与应用生物学,2018,37(12):5251-5257.DOI:10.13417/j.gab.037.005251.
基金信息:
广东省科技计划(2014A020208122);; 广东省教育厅重大培育项目(2014GKXM049);; 广东省海洋渔业科技专项(Z2014009和B201601-Z10);; 国家贝类产业体系专项(CRAS-047)共同资助
2017-09-21
2017-09-21
2017-09-21