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通过改良解磷菌固体分离培养基的分离培养,从‘凤丹’牡丹(Paeonia ostii‘Fengdan’)根际土壤中分离到3株具有解磷活性的菌株,编号分别为YF19、YF20和YF21。通过74 h的连续发酵培养考察了三株菌的解磷活性,其中YF20菌株的解磷活性最强,发酵48 h时,发酵上清液中有效磷含量达到最大值1.35 mg/L。综合形态特征观察、生化测定和16S r DNA分析结果,确定菌株YF20隶属于鞘鞍醇杆菌属,暂命名为Novosphingobium sp.YF20。生长特性研究表明,菌株YF20对6种碳源的同化能力由强到弱的顺序是:柠檬酸钠>蔗糖>淀粉>乙酸钠>葡萄糖>乳糖;对6种氮源的同化能力由强到弱的顺序是:蛋白胨>酵母膏>硝酸钠>氯化铵>硝酸钾>硫酸铵;最适生长温度为32~36℃,最适p H值范围是6.06.5。菌株YF20可作为油用牡丹专用菌肥开发的出发菌株。
Abstract：3 kinds of strains with activity of phosphate solubilizing was isolated from the rhizosphere soil of Paeonia ostii by culture of the modified phosphate solid medium, and their numbers were YF19, YF20 and YF21,respectively. After 74 h continuous fermentation culture, the hydrolysis activity of three strains was investigated.YF20 strain had the best phosphate solubilizing activity, and the effective phosphorus content in the fermentation supernatant reached the maximum value of 1.35 mg/L during the fermentation of 48 h. By comprehensive results of morphological observation, biochemical assay and 16 S r DNA analysis, we initially identified YF20 belonged to Novosphingobium genus, and tentatively named after Novosphingobium sp. YF20. The growth characteristics study showed that the assimilation ability of strain YF20 to 6 kinds of carbon sources from strong to weak was sodium citrate >sucrose >starch >sodium acetate >glucose >lactose. The order of assimilation ability to 6 kinds of nitrogen sources from strong to weak was peptone >yeast extract >sodium nitrate >ammonium chloride >potassium nitrate >ammonium sulfate. In addition, the optimum growth temperature was 32~36℃, and the range of optimum p H value was 6.06.5. Strain YF20 could be used as strain to special fertilizer development of oil peony.
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基本信息:
DOI：10.13417/j.gab.036.005173
中图分类号:S154.3
引用信息:
[1]孙会忠,宋月芹,乔琦,等.解磷菌Novosphingobiumsp.YF20的分离及生长特性[J].基因组学与应用生物学,2017,36(12):5173-5178.DOI:10.13417/j.gab.036.005173.
基金信息:
河南省教育厅高等学校重点科研项目(16A180003);; 河南省科技厅农业公关计划项目(172102110020);; 河南科技大学学科提升振兴A计划项目(13660001)共同资助
2017-12-25
2017-12-25