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采用不连续聚丙烯酰胺凝胶电泳技术对楸树体细胞胚胎发生过程中的酯酶(EST)、过氧化物酶(POD)、淀粉酶(AMY)及ATP酶4种同工酶进行分析。结果表明,EST及POD同工酶酶带在楸树体细胞胚胎发生不同时期呈现规律性变化,胚性愈伤组织中EST、POD同工酶酶带较非胚性愈伤组织多且表达活跃,子叶胚时期活性最强,表明这一时期细胞内代谢旺盛。EST、POD、AMY及ATP同工酶在楸树胚性与非胚性愈伤组织中谱带差异明显,表明这4种同工酶与体胚发生具有密切关系,可以作为楸树胚性愈伤组织和体胚发生的重要标志。
Abstract：The non-denatured polyarcrylamide gel electrophoresis(PAGE) technology was applied to analyze four isoenzymes such as esterase(EST),peroxidase(POD),amylase(AMY) and ATPase in somatic embryogenesis of Catalpa bungei C. A. Mey.. The results showed that regular variations of EST and POD isoenzyme activities were presence with the development of somatic embryos,isoenzyme strands of EST and SOD in embryogenic callus tissue were more than that of non-embryogenic callus tissue,and a higher expression of these two isoenzymes in cotyledon embryo indicated that vigorous metabolism was proceeding in this period. Moreover,observed significant differences of EST,POD,AMY and ATP isoenzymes shared close relationship in somatic embryogenesis,and can be regarded as an important reference label for each specific developmental stage.
Du J.C.,Jing D.L.,Chen F.J.,and Li F.L.,2010,6 kinds o isozymes after long-term subculture of Emmenopterys henry Oliv.,Agri.Sci.Tech.,11(5):22-27
He Z.X.,and Zhang S.Z.,eds.,1999,Electrophoresis,Scienc Press,Beijing,China,pp.37-38,286-300(何忠效,张树政,主编,1999,电泳,科学出版社,中国,北京,pp.37-38,286-300)
Jiang C.R.,Peng F.R.,and Tan P.P.,2014,Somatic embyrogene sis and the physiological and biochemical characteristics in Catalpa fargesii Bur.f.duclouxii(Dode)Gilmour,Linye Keji Kaifa(China Forestry Science and Technology),28(1)25-29(江翠荣,彭方仁,谭鹏鹏,2014,滇楸体胚发生及生理生化特性研究,林业科技开发,28(1):25-29)
Kochba J.,Lavee S.,and Spiegel-Roy P.,1977,Differences in peroxidase activity and isoenzymes in embryogenic are non-embryogenic‘Shamouti’orange ovular callus lines Plant Cell Physiol.,18(2):463-467
Lai H.G.,Li R.M.,Wang J.,Fu S.P.,Hu X.W.,Zhang P.,and Guo J.C.,2011,Esterase and amylase isozymograms during somatic embryogenesis in cassava(Manihot esculenta Crantz SC5 and SC6,Redai Zuowu Xuebao(Chinese Journal of Tr opical Crops),32(7):1201-1205(赖杭桂,李瑞梅,王静,符少萍,胡新文,张鹏,郭建春,2011,华南5号和华南6号木薯体胚发生过程中酯酶和淀粉酶同工酶分析,热带作物学报,32(7):1201-1205)
Li S.M.,Li M.,and Tian J.H.,2014,Influences of different cul ture times on zymogram of esterase isozyme of Pleurotu eryngii,Beifang Yuanyi(Northern Horticulture),(14):98-100(李守勉,李明,田景花,2014,不同培养时间对杏鲍菇酯酶同工酶酶谱的影响,北方园艺,(14):98-100)
Olsson M.,1995,Alterations in lipid composition,lipid peroxida tion and anti-oxidative protection during senescence in drought stressed plants and non-drought stressed plants o Pisum sativum,Plant Physiol.Bioch.,33(5):547-553
Reng Y.,Peng F.R.,Yang Y.,and Wang L.F.,2011,Morphologi cal and cytological observation on the different callus in Catalpa bungei,Linye Keji Kaifa(China Forestry Science and Technology),25(5):43-45(任莺,彭方仁,杨燕,王列富2011,楸树不同类型胚性与非胚性愈伤组织的细胞学观察,林业科技开发,25(5):43-45)
Su B.,Wang Y.X.,Lan H.,Wang W.,and Zhang Y.P.,1999Phylogenetic study of complete cytochrome b gene in musk deer(Genus moschus)using museum samples,Mol.Phylo genet Evol.,12(3):241-249
Tang L.,Jiang W.B.,and Weng M.L.,2007,Landscape charac ters of Catalpa bungei and their exploitation and application Yuanyi Yuanlin Kexue(Chinese Agricultural Science Bul letin),23(4):276-280(唐玲,姜卫兵,翁忙玲,2007,楸树的园林特性及其开发应用,园艺园林科学,23(4):276-280)
Wang L.H.,Wang X.,Cui K.R.,Jiao C.J.,and Wang Y.F.,1996Starch metabolism during somatic embryogenesis of Aspa ragus officinalis and Codonopsis pilosula,Zhiwuxue Tong bao(Chinese Bulletin of Botany),13(1):41-45(汪丽虹,王星,崔凯荣,焦成瑾,王亚馥,1996,石刁柏及党参胚状体发生中的淀粉代谢动态,植物学通报,13(1):41-45)
Wei Q.,Cao Y.L.,Chen F.,Zhou L.J.,and Chen D.L.,2000,Ob servation of somatic embryogenesis and analyses of peroxi dase isozymes in culture of the pith of Lycium barbarum L.Guangxi Zhiwu(Guihaia),20(2):168-171(魏琴,曹有龙,陈放,周黎军,陈东林,2000,枸杞髓组织培养中体细胞胚胎发生与过氧化物酶同工酶分析,广西植物,20(2)168-171)
Xiong D.,Chen F.J.,Liang H.W.,Wang Y.B.,and Li X.L.,2008,Studies on four kinds of isozymes during somatic embryogenesis of Emmenopterys henryi Oliv.,Xibei Zhiwu Xuebao(Act Botanica Boreali-Occidentalia Sinica),28(10):2011-2016(熊丹,陈发菊,梁宏伟,王玉兵,李晓玲,2008,香果树体细胞胚胎发生过程中4种同工酶的研究,西北植物学报28(10):2011-2016)
Yang J.L.,Gui Y.L.,and Guo Z.C.,1998,Studies on cytohistolo gy and starch accumulation during somatic embryogenesi of Picea meyeri rehd.et.Wils.,Xibei Zhiwu Xuebao(Act Botanica Boreali-Occidentalia Sinica),18(3):335-338(杨金玲,桂耀林,郭仲琛,1998,白杄体细胞胚胎发生的细胞组织学和淀粉积累动态的研究,西北植物学报,18(3):335-338)
Zhai X.Q.,Zhang S.,and Fan G.Q.,2006,Peroxidase isozyme changes during somatic embryogenesis in Paulownia,Henan Kexue(Henan Science),24(1):56-59(翟晓巧,张胜,范国强,2006,泡桐体细胞胚胎发生过程中过氧化物酶的变化河南科学,24(1):56-59)
Zhan Y.F.,Wu Z.,Jin X.X.,and Wang G.D.,2006,Anti-oxida tive enzymes and some physiological features in somati embryogenesis of garlic,Xibei Zhiwu Xuebao(Acta Botani ca Boreali-Occidentalia Sinica),26(9):1799-1802(詹园凤吴震,金潇潇,王广东,2006,大蒜体细胞胚胎发生过程中抗氧化酶活性变化及某些生理特征,西北植物学报,26(9):1799-1802)
Zhang B.,Jing D.L.,Li X.L.,Wang Y.B.,and Zhang D.C.,2011Studies on isozymes during somatic embryogenesis o Tapiscia sinensis,Guangxi Zhiwu(Guihaia),31(4):526-530(张博,景丹龙,李晓玲,王玉兵,张德春,2011,珍稀濒危植物银鹊树体细胞胚时期同工酶分析,广西植物,31(4)526-530)
Zhao X.Y.,Wang J.H.,Zhang J.F.,Zhang S.G.,Zhang J.G.,and Ma J.W.,2008,Study on phenotypic traits and germination characters of four taxons of Catalpa genus seed,Xibe Nonglin Keji Daxue Xuebao(Journal of Northwest A&F Un iversity),36(12):149-154(赵曦阳,王军辉,张金凤,张守攻张建国,马建伟,2008,梓树属4个种种子表型性状和发芽特性的研究,西北农林科技大学学报,36(12):149-154)
基本信息:
DOI：10.13417/j.gab.035.003122
中图分类号:S792.99;Q946.5
引用信息:
[1]王长兰,张青,魏文桃,等.楸树体细胞胚胎发生过程中4种同工酶分析[J].基因组学与应用生物学,2016,35(11):3122-3127.DOI:10.13417/j.gab.035.003122.
基金信息:
国家“十二五”科技支撑计划课题项目(2012BAD21B03)资助
2016-11-25
2016-11-25