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从泸州老窖酒厂的酒糟和窖泥中采集的168个样品中分离出苏云金芽孢杆菌1株,经光学显微镜观察伴孢晶体形态为菱形。以cry1/7/9、cry2、cry3、cry5、cry6、cry8、cry4/10、cry11、cry1Ⅰ的引物做PCR分析进行基因型鉴定,发现该分离株有cry1基因。同时还对该株蛋白质图谱和杀虫活性等特性分析,结果表明该分离株对小菜蛾有一定活性,死亡率可达80%,但该菌株对致倦库蚊无活性。
Abstract：One Bacillus thuringiensis strain was isolated from 168 grain and pit mud samples, which were collected in Luzhou distillers'. Microscopic analysis showed that the shape of its parasporal crystal was rhombus. The genotype was analysized by PCR using primer of cry1/7/9, cry2, cry3, cry5, cry6, cry8, cry4/10, cry11 and cry1Ⅰ, and only cry1 was detected. Meanwhile, the characteristics of protein pattern and insecticidal activity of this new isolate were also tested. Bioassay indicated this isolate was toxic to lepiteran pest diamondback moth, with mortality of 80%. However, it was non-toxic to dipteran pest Culex quinquefasciatus.
Crickmore N.,Zeigler D.R.,Feitelson J.,Schnepf E.,van Rie J.,Lereclus D.,Baum J.,and Dean D.H.,1998,Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins,Microbiol&Mol.Biol.Rev.,62(3):807-813
Gao L.,2004,Comprehensive utilization of distiller's grains,Niangjiu Keji(Liquor-Making Science&Technology),125(5):101-102(高路,2004,酒糟的综合利用,酿酒科技,125(5):101-102)
Iriarte J.,Porcar M.,Lecadet M.M.,and Caballero P.,2000,Iso-lation and cordially,haracterization of Bacillus thuringiensis strains from aquatic environments in Spain,Current Microbiology,40(6):402-408
Kuo W.S.,and Chak K.F.,1996,Identification of novel cry-type genes from Bacillus thuringiensis strains on the basis of restriction fragment length polymorphism of the PCR-amplified DNA,Appl.Environ.Microbiol.,62(4):1369-1377
Li S.L.,Zhu Y.W.and Li X.S.,2008,Application of manmade pit mud,Niangjiu Keji(Liquor-Making Science&Technology),173 (11):87-89(李绍亮,朱云武,李学思,2008,人工培养窖泥的应用,酿酒科技,173(11):87-89)
Luo L.,Fang F.,Lin J.,Guan C.Y.,and Zhang L.L.,2005,Progress of studies on isolating Bacillus thuringiensis(Bt)from the plant leaf surface,Huandong Kunchong Xuebao(Entomological Journal of East China),14(4):348-352(骆兰,方昉,林晶,关春宇,张灵玲,2005,叶面分离苏云金芽孢杆菌的研究进展,华东昆虫学报,14(4):348-352)
Luo L.,Xie B.Y.,Yuan Z.L.,and Yang Z.W.,2005,Identification of cry-type gene and isolation of Bacillus thuringiensis from soil in China,Yingyong Yu Huanjing Shengwu Xuebao(Chinese Journal Applied and Environmental Biology),11(6):756-759(罗兰,谢丙炎,袁忠林,杨之为,2005,我国土壤中苏云金芽孢杆菌的分离与基因型的鉴定,应用与环境生物学报,11(6):756-759)
Maeda M.,Mizuki E.,Nakamura Y.,Hatano T.,and Ohba M.2000,Recovery of Bacillus thuringiensis from marine sediments of Japan,Current Microbiology,40(6):418-422
Mizuki E.,Maeda M.,Tanaka P.,Lee D.W.,Hara M.,Akao T.,Yamashita S.,Kim H.S.,Ichimatsu T.,and Ohba M.,2001,Bacillus thuringiensis:A common member of microflora in activated sludges of a sewage treatment plant,Current Microbiology,42(6):422-425
Morris O.N.,Kanaaratnam P.,and Converse V.,1997,Suitability of 30 aricultural and by-products as nutrient sources for laboratory prodution of Bacillus thuringiensis subup.Aizawai(HD133),J.Invert.Pathol.,70(2):113-120
Sambrook J.,and Russell D.V.,eds.,Huang P.T.,trans.,2002,Molecular cloning:A laboratory manual,Science Press,Beijing,China,pp.422-424
Poopathi S.,and Kumar K.A.,2003,Novel fermentation media for production of Bacillus thuringiensis subsp.Israelensis,J.Econ.Entomol.,96(4):1039-1044
Wu L.Y.,He J.Q.,Zhou Y.,Chen G.R.,Zhang L.,Zhang Y.R.,Wu S.Q.,and Guan X.,2010,Isolation and genotype identification of Bacillus thuringiensis from waste water and sludge,Fujian Nonglin Daxue Xuebao(Journal of Fujian Agriculture and Forestry University(Natural Science Edition)),39(1):19-24(吴丽云,何金清,周勇,陈国荣,张琳,张永嵘,吴松青,关雄,2010,污水,污泥中苏云金杆菌的分离及其基因型鉴定,福建农林大学学报,39(1):19-24)
Xu Z.C.,2001,Thought of the development of liquor-making in dustryin China,Niangjiu Keji(Liquor-making Science&Technology),108(6):24-25(徐占成,2001,对发展中国白酒行业的思考,酿酒科技,108(6):24-25)
Zhang L.L.,Lin J.,Luo L.,Fang F.,Huang T.P.,Xu J.H.,Wu G.Y.,Wang Q.S.,and Guan X.,2005,Occurrence of Bacillus thuringiensis on the phylloplane and screening of highly toxic strains to tea pests,Chaye Kexue(Journal of Tea Science),25 (1):56-60(张灵玲,林晶,骆兰,方昉,黄天培,徐金汉,吴光远,王庆森,关雄,2005,叶面分离Bt及对茶树主要害虫高毒力菌株的筛选,茶叶科学,25(1):56-60)
基本信息:
DOI：10.13417/j.gab.033.000293
中图分类号:TS261.1
引用信息:
[1]郑琦,邓波,关雄,等.酒糟和窖泥中苏云金芽孢杆菌的分离及鉴定[J].基因组学与应用生物学,2014,33(02):293-298.DOI:10.13417/j.gab.033.000293.
基金信息:
国家863项目(2011AA10A203);; 泸州老窖技术开发项目(2013K-116))共同资助
2014-04-28
2014-04-28