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为研究龙牙百合多糖(LP)对人肝癌HepG2细胞抑制和凋亡的影响,采用MTT法考察了3组LP不同浓度对人肝癌HepG2细胞抑制作用,采用Annexin-V/PI双染法和Western blotting法探究了LP1和LP42个组分对人肝癌HepG2细胞凋亡作用机制。LP对人肝癌HepG2细胞抑制呈剂量依赖关系,LP4组1 mg/mL对HepG2细胞的抑制率达46.36%,效果最佳;LP1组8 mg/mL最高抑制率达43.23%;LP2组4 mg/mL最高抑制率达24.35%;LP1和LP4对HepG2细胞的晚期凋亡率显著高于早期。激活Caspase-3、Bax、蛋白表达显著升高(p<0.05),Bcl-2蛋白表达水平明显降低(p<0.05)。研究表明,LP通过激活死亡受体凋亡途径和线粒体途径,诱导人肝癌HepG2细胞凋亡。
Abstract：In order to study the effects of Lilium polysaccharides(LP) on the inhibition and apoptosis induced in HepG2 cells, MTT assay was used to perform the inhibitory effect on HepG2 cells at certain tme intervals after incubation of various concentrations of LP. The protein levels of apoptosis in HepG2 cells with LP1 and LP4 were detected by Western blotting. Our research shows that LP reduced viability and proliferation of the HepG2 cells dose-dependently to some extent. The best inhibition rate of HepG2 cells was 46.36% in LP4 group with 1 mg/mL,followed by the highest inhibition rate of 43.23% in LP1 group with 8 mg/mLand the highest inhibition rate of 24.3% in LP2 group with 4 mg/mL. The late apoptotic rate of LP1 and LP4 cells was significantly higher than that of early apoptotic cells. Up-regulated the ratio of caspase-3, Bax, and down-regulated the ratio of Bcl-2 after treatment with SP. Above study, LP induces apoptosis of human HepG2 cells by activating death receptor apoptosis pathway and mitochondrial pathway.
Altekruse S.F.,Mc Glynn K.A.,and Reichman M.E.,2009,Hepatocellular carcinoma incidence,mortality,and survival trends in the United States from 1975 to 2005,J.Clin.Oncol.,27(9):1485-1491
Hou J.,Li F.,Li X.H.,Mei Q.B.,and Mi M.,2016,Lily polysaccharide 1 enhances the effect of metformin on proliferation and apoptosis of human breast carcinoma cells,Xibao Yu Fengzi Mianyixue Zazhi(Chinese Journal of Cellular and Molecular Immunology),32(6):780-788(侯进,李汾,李新华,梅其炳,弥曼,2016,百合多糖1可增强二甲双胍对乳腺癌细胞的抗增殖作用并促进其凋亡,细胞与分子免疫学杂志,32(6):780-788)
Hou J.,Zhu Y.X.,Li Y.,and Zhang B.L.,2017,Synergistic antitumor activity of lily polysaccharide combined with metformin on human liver cancer HepG2 cells,Liaoning Zhongyiyao Daxue Xuebao(Journal of Liaoning University of Traditional Chinese Medicine),19(6):30-32(侯进,朱永香,李宇,张冰楠,2017,百合多糖与二甲双胍联用对人类肝癌细胞HepG2株抑制作用,辽宁中医药大学学报,19(6):30-32)
Justin K.,Elizabeth J.O.,and David W.A.,2018,BCL-2 family proteins:Changing partners in the dance towards death,Cell Death.Differ.,25(1):65-80
Li H.Q.,Wu F.F.,Long Y.Z.,Zhao L.Z.,Xu Y.P.,and Li S.Y.,2017,In vitro antioxidative effects of Longhui Longya lilium polysaccharides,Shiping Anquan Zhiliang Jiance Xuebao(Journal of Food Safety&Quality),8(8):3068-3073(李化强,吴菲菲,龙艳珍,赵良忠,徐永平,李淑英,2017,隆回龙牙百合多糖的体外抗氧化活性研究,食品安全质量检测学报,8(8):3068-3073)
Li F.,Yuan B.X.,Mi M.,Ren L.J.,Li X.H.,Wang D.L.,and Wang Y.,2008,Effects of purified polysaccharides from Lily on tumor growth and immune functions of tumor-bearing mice,Xiandai Zhongliu Yixue(Modern Oncology),116(2):188-189(李汾,袁秉祥,弥曼,任利君,李新华,王多宁,王颖,2008,纯化百合多糖抗肿瘤作用和对荷瘤小鼠免疫功能的影响,现代肿瘤医学,116(2):188-189)
Mise Y.,Sakamoto Y.,Ishizawa T.,Kaneko J.,Aoki T.,Hasegawa K.,Sugawara Y.,and Kokudo N.,2013,A worldwide survey of the current daily practice in liver surgery,Liver Cancer,2(1):55-66
Reed J.C.,2008,Bcl-2 family proteins and hematologic malignancies:History and future prospects,Blood,111:3322-3330
Yu Q.S.,Yang L.,Zhang C.,and Peng G.P.,2017,Optimization of extraction process of polysaccharide from lilium brownii,Human Nongye Kexue(Hunan Agricultural Sciences),(5):84-86(余倩莎,杨岚,张城,彭国平,百合多糖提取工艺优化,湖南农业科学,(5):84-86)
基本信息:
DOI：10.13417/j.gab.039.002374
中图分类号:R285.5
引用信息:
[1]杨岚,余倩莎,彭逸斯,等.龙牙百合多糖对人肝癌细胞HepG2凋亡的影响[J].基因组学与应用生物学,2020,39(05):2374-2378.DOI:10.13417/j.gab.039.002374.
基金信息:
国家自然科学基金(NO.31471446);; 道地药用植物规范化栽培与综合开发湖南省工程实验室专项补助资金共同资助
2019-02-27
2019-02-27
2019-02-27