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本研究旨在探讨运动强度对2型糖尿病血流动力学和能量代谢的影响。将SD大鼠随机分为对照组、模型组、低强度组和中强度组。通过高脂饮食和注射链脲佐菌素(50 mg/kg)来建立2型糖尿病大鼠模型。建模后,低强度组和中强度组大鼠分别按照10 m/min和15 m/min的速度进行跑台运动,跑台坡度为0°,时间为30 min,每周5 d,共进行4周。运动后检查各组大鼠的心功能参数(LVEDD, LVESD和LVEF)、血流动力学指标(SBP, DBP和MAP)、血脂代谢指标(TG, TC, HDL-C和LDL-C)和能量代谢指标(Glu, Lac, BUN和NEFA)。通过Periodic-acid-schiffs (PAS)染色和天狼星红染色评价大鼠肾脏组织病变。研究显示,中强度组的LVEDD和LVESD显著低于低强度组,而LVEF显著高于低强度组。中强度组的SBP、DBP和MAP水平显著低于低强度组。与低强度组相比,中强度组的TG、TC和LDL-C水平均显著降低,而HDL-C水平显著升高。与低强度组相比,中强度组的Glu、Lac、BUN和NEFA水平显著降低。2型糖尿病大鼠近曲肾小管细胞扩张,血管变性明显,肾小球扩大,肾脏组织间隙胶原蛋白显著增加,肾脏纤维化明显。而两个运动组大鼠肾脏组织病变明显减轻。中强度组的GLUT-4和IRS-1蛋白表达水平显著高于低强度组。本研究表明,运动可有效改善2型糖尿病的血流动力学、能量代谢和脂代谢。在承受范围之内适当提高运动强度更有利于2型糖尿病患者的康复。
Abstract：This study aims to explore the effects of exercise intensity on hemodynamics and energy metabolism in type 2 diabetes. SD rats were randomly divided into control group, model group, low-intensity group and medium-intensity group. A high fat diet and injection of streptozotocin(50 mg/kg) were used to establish a rat model of type 2 diabetes. After the modeling, the rats in the low-intensity group and the medium-intensity group performed treadmill exercise at a speed of 10 m/min and 15 m/min, respectively. The slope of running platform was 0°, the time was 30 minutes, 5 days a week, a total of 4 weeks. After exercise, cardiac function parameters(LVEDd, LVESD and LVEF), hemodynamic indexes(SBP, DBP and MAP), lipid metabolism indexes(TG, TC,HDL-C and LDL-C) and energy metabolism indexes(Glu, LAC, bun and NEFA) were examined. Rat kidney tissue lesions were evaluated by periodic-acid-schiffs(PAS) staining and Sirius Red staining. Studies showed that LVEDD and LVESD in the medium intensity group are significantly lower than those in the low intensity group,while LVEF is significantly higher than that in the low intensity group. The levels of SBP, DBP and MAP in the medium intensity group were significantly lower than those in the low intensity group. Compared with the low-intensity group, the levels of TG, TC, and LDL-C in the medium-intensity group were significantly reduced,while the HDL-C levels were significantly increased. Compared with the low-intensity group, the levels of Glu,Lac, BUN, and NEFA were significantly reduced in the medium-intensity group. In type 2 diabetic rats, the proximal tubule cells expanded, vascular degeneration was obvious, the glomerulus was enlarged, the collagen in the renal tissue space was significantly increased, and renal fibrosis was obvious. The kidney tissue lesions of the two exercise groups were significantly reduced. The expression levels of GLUT-4 and IRS-1 proteins in the medium intensity group were significantly higher than those in the low intensity group. This study indicates that exercise can effectively improve the hemodynamics, energy metabolism and lipid metabolism of type 2 diabetes.Properly increasing exercise intensity within the tolerance range is more conducive to the rehabilitation of patients with type 2 diabetes.
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基本信息:
DOI：10.13417/j.gab.039.004270
中图分类号:R587.1
引用信息:
[1]赵昆,黎璞.运动强度对2型糖尿病血流动力学和能量代谢的影响[J].基因组学与应用生物学,2020,39(09):4270-4276.DOI:10.13417/j.gab.039.004270.
基金信息:
四川省科技厅支撑计划项目(2014SZ0107)资助
2020-09-25
2020-09-25