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为探讨当归多糖(angelica polysaccharide, AP)对免疫性卵巢早衰小鼠内分泌功能的影响及分子机制,本研究将60只雌性BALB/c小鼠分为正常组、模型组、AP低剂量(100 mg/kg)、中剂量(200 mg/kg)、高剂量(400 mg/kg)组和补佳乐阳性组,以小鼠透明带多肽为抗原,皮下多点注射建立免疫性卵巢早衰(premature ovarian failure, POF)小鼠模型,灌胃相应药物,连续治疗4周后检测各组小鼠卵巢指数,检测各组小鼠血液SOD和MDA水平;通过ELISA检测各组小鼠卵巢组织IL-1β和IL-6水平,Western blotting检测各组小鼠p-AKT和FOXO3蛋白表达水平。与空白组相比,模型组小鼠卵巢指数显著降低,与模型组比较,AP低、中、高剂量组和阳性组小鼠卵巢指数显著升高,差异具有统计学意义(p<0.01)。与空白组比较,模型组小鼠血液SOD活性显著降低,MDA含量显著升高,与模型组比较,AP低、中、高剂量组和阳性组小鼠血液SOD活性显著升高,MDA含量显著降低。ELISA结果显示,与空白组比较,模型组小鼠卵巢组织IL-1β和IL-6含量显著升高,与模型组比较,AP低、中、高剂量组小鼠卵巢组织IL-1β和IL-6含量显著降低,差异具有统计学意义(p<0.01)。Western blotting结果显示,与空白组比较,模型组小鼠卵巢p-AKT和FOXO3蛋白表达显著降低,与模型组比较,AP低、中、高剂量组和阳性组小鼠卵巢p-AKT和FOXO3蛋白表达显著升高,差异具有统计学意义(p<0.01)。本研究结果说明,当归多糖AP激活AKT/FOXO3通路,抑制小鼠氧化应激,改善免疫性卵巢早衰小鼠内分泌功能,可在一定程度上抑制免疫卵巢早衰。
Abstract：To investigate the effect of Angelica polysaccharide(AP) on endocrine function in and its molecular mechanism in mice with immune premature ovarian failure. This study divided sixty female BALB/c mice into normal group, model group, AP low dose(100 mg/kg), medium dose(200 mg/kg), high dose(400 mg/kg) group and Bujiale positive group. The mouse model of immune premature ovarian failure(POF) was established by subcutaneous multipoint injection with a mouse zona pellucida polypeptide as an antigen. The corresponding drugs were intragastrically administered. The ovarian index and the levels of SOD and MDA in blood of mice in each group was detected after 4 weeks of continuous treatment. The levels of IL-1β and IL-6 in ovarian tissue of mice in each group were detected by ELISA. The expression levels of p-AKT and FOXO3 protein of mice in each group were detected by Western blotting. Compared with the blank group, the ovarian index of the model group decreased significantly. Compared with the model group, the ovarian index of the low, medium and high dose groups of AP and the positive group significantly increased, and the difference was statistically significant( p<0.01).Compared with the blank group, the blood SOD activity of the model group significantly decreased, and the MDA content significantly increased. Compared with the model group, the blood SOD activity of the low, medium and high dose groups of AP and the positive group significantly increased, and the MDA content significantly decreased. The results of ELISA showed that the contents of IL-1β and IL-6 in the ovarian tissue of the model group were significantly higher than those in the blank group. Compared with the model group, the contents of IL-1β and IL-6 in the ovarian tissue of the low, medium and high dose groups of AP significantly decreased, and the difference was statistically significant(p<0.01). Western blotting results showed that compared with the blank group, the expression of p-AKT and FOXO3 protein in the ovary of mice in model group was significantly lower. Compared with the model group, the the expression of p-AKT and FOXO3 protein in ovaries of mice in the low, medium and high dose groups of AP and the positive group significantly increased, and the difference was statistically significant( p<0.01). AP activated AKT/FOXO3 pathway, inhibited oxidative stress in mice, and improved endocrine function in mice with immune ovarian premature failure, which would inhibit immune premature ovarian failure.
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基本信息:
DOI：10.13417/j.gab.038.003268
中图分类号:R285.5
引用信息:
[1]李莉,卢鹏,高霞.当归多糖通过AKT/FOXO3通路调节免疫性卵巢早衰小鼠内分泌功能[J].基因组学与应用生物学,2019,38(07):3268-3272.DOI:10.13417/j.gab.038.003268.
2019-07-25
2019-07-25