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为探究大豆肽对凡纳滨对虾肠道菌群影响以及肠道菌群的变化与生长的相互关系,本实验配制大豆肽替代鱼粉量分别为0%(FM),20%(FSM20),100%(FSM100)的3种等氮等能饲料。实验设置共分3组,每组3个重复,凡纳滨对虾初重为(1.53±0.36) g,在水泥池中的网箱养殖50 d。实验结束后,每组取15尾虾,利用高通量测序等原理,对肠道微生物进行测序分析。结果表明,大豆肽对凡纳滨对虾的生长性能影响显著,全鱼粉组FM、增重率、特定生长率等指标显著高于全替代组FSM100 (p<0.05),但与FSM20组差异不显著(p>0.05);大豆肽对肠道菌群的影响与大豆肽的量有重要关系。FM组与FSM100组菌落组成有显著的差异,但FM组与FSM20组差异不显著。全大豆肽组中,螺旋体纲(Spirochaetes)的数量会有显著下降,γ-变形菌纲(Gamma proteobacteria)占比明显上升,肠道微生物多样性显著下降(p<0.05)。凡纳滨对虾FSM100组生长性能显著低于全鱼粉组FM,可能与肠道菌群中螺旋体纲与γ-变形菌纲数量的变化有关。
Abstract：This study aimed to explore the effects of soy peptide on the intestinal flora of Litopenaeus Vannamei and the relationship between the changes and growth of intestinal flora. In this experiment, three kinds of isonitrogenous and isoenergetic experimental diets were formulated by replacing fish meal with soy peptide by 0%(FM),20%(FSM20) and 100%(FSM100). The experimental shrimps were divided into 3 groups, with 3 replicates in each group. The shrimps with an initial weight of(1.53±0.36) g were fed the three test diets for 50 days. At the end of the experiment, 15 shrimps were taken from each group, and the intestinal microbes were sequenced by high-throughput sequencing. The results showed that soy peptide had significant effects on the growth performance of Litopenaeus Vannamei. The weight gain rate and specific growth rate of FM were significantly higher than FSM100(p<0.05), but the difference was not significant with the FSM20(p>0.05). The effects of soy peptide on intestinal flora is related to the amount of soy peptide. Here was a significant difference in the colony composition between the FM group and the FSM100 group, but the difference between the FM group and the FSM20 group was not significant. In the FSM100 group, the number of Spirochaetes decreased significantly, the proportion of γ-Proteobacteria increased significantly, and the microbial diversity of intestinal tract decreased significantly(p<0.05). The growth performance of FSM100 group was significantly lower than that FM group, which may be related to the change of the number of spirochetes and γ-proteobacteria in the intestinal microflora.
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基本信息:
DOI：10.13417/j.gab.039.005084
中图分类号:S968.22
引用信息:
[1]张彦俊,石磊,谢天,等.大豆肽替代鱼粉对凡纳滨对虾肠道菌群的影响[J].基因组学与应用生物学,2020,39(11):5084-5090.DOI:10.13417/j.gab.039.005084.
基金信息:
凡纳滨对虾功能性饲料的开发与应用项目(ZX-20180101)资助
2020-11-25
2020-11-25