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巴西橡胶中Rop家族基因能调控植物小G蛋白合成,是分子信号开关,参与橡胶树刮伤诱导乳管分化、防御胁迫应答和胶乳再生。为了揭示巴西橡胶树HbRop基因家族5个成员在细胞核染色体上的实际位置,展现家族基因之间的分布特点和连锁遗传关系,丰富橡胶树分子细胞遗传学信息,为橡胶树的分子辅助育种和比较基因组学研究提供分子细胞遗传学的科学理论依据。本研究以巴西橡胶树‘热研7-33-97’品种为材料将HbRop基因家族5个成员(HbRop1, HbRop2, HbRop3, HbRop4, HbRop5)定位在细胞核染色体上,通过双探针荧光原位杂交技术对橡胶树Rop小G蛋白基因家族5个成员在细胞核染色体上进行物理定位分析。实验结果表明:HbRop1基因定位在第1号染色体的短臂上,其信号位点到着丝粒的平均百分距离是63.34;HbRop2、HbRop3、HbRop4和HbRop5分别定位在第4、第3、第7和第10号染色体的长臂上,这些基因的信号位点到对应染色体着丝粒的平均百分距离分别是25.13、44.68、44.33和17.46,同时还讨论了它们与其他已定位的基因的位置关系。HbRop基因家族5个基因分别位于不同的染色体上,彼此间不存在连锁现象。
Abstract：Hevea brasiliensis Rop family genes can regulate the synthesis of small G protein in plants, which is a molecular signal switch involved in Hevea brasiliensis scratching induced milk duct differentiation, defense stress response and latex regeneration. In order to reveal the actual location of five members of the HbRop gene family of Hevea brasiliensis on the nuclear chromosome, to reveal the distribution characteristics and linkage genetic relationships between family genes, to enrich the cytogenetic information of Hevea brasiliensis molecules,molecular assisted breeding of Hevea brasiliensis and comparative genomics research Provide scientific theoretical basis for molecular cytogenetics. In this study, we located five members of the HbRop gene family(HbRop1,HbRop2, HbRop3, HbRop4, HbRop5) on chromosomes of Hevea brasiliensis, in order to provide reference for the homing of the scaffold on the chromosomes of the Hevea brasiliensis, and to provide a scientific basis for the molecular assisted breeding and comparative genomics of Hevea brasiliensis. In this study, Hevea brasiliensis‘Reyan 7-33-97'was used as the material to carry out physical location analysis on the nuclear chromosome of the rubber tree Rop small G protein gene family by dual-probe fluorescence in situ hybridization. The experimental results show that the HbRop1 gene was located on the short arm of chromosome 1 and the mean percentage distance from the signaling site to the centromere is 63.34; HbRop2, HbRop3, HbRop4 and HbRop5 are located on the long arms of chromosomes 4, 3, 7 and 10, respectively. The mean percentage distances from the signal points of these genes to the corresponding centromeres are 25.13, 44.68, 44.33 and 17.46, respectively their positional relationship with other localized genes was also discussed. The 5 gene sequences of the HbRop gene family located on different chromosomes, and there is no linkage phenomenon.
Cao Y.R.,Li Z.G.,Chen T.,Zhang Z.G.,Zhang J.S.,and Chen S.Y.,2008,Overexpression of a tobacco small G protein gene Nt Rop1 causes salt sensitivity and hydrogen peroxide production in transgenic plants,Science in China,51(5):383-390
Chan J.,and Peter K.P.,2007,Brassica napus Rop GTPases and their expression in microspore cultures,Planta,225(2):469-484
Chen L.,Shiotani K.,Togashi T.,Miki D.,Aoyama M.,Wong H.L.,Kawasaki T.,and Shimamoto K.,2010,Analysis of the Rac/Rop small GTPase family in rice:expression,subcellular localization and role in disease resistance,Plant and Cell Physiology,51(4):585-595
Gao H.Q.,Zhuang N.S.,Wang Y.,and Qiu H.Y.,2009,Karyotype analysis of two varieties in Hevea brasiliensis,Wuhan Zhiwyxue Yanjiu(Journal of Wuhan Botanical Research),27(5):537-540(高和琼,庄南生,王英,邱海燕,2009,橡胶树两个品种的核型分析,武汉植物学研究,27(5):537-540)
Gong L.,Chen P.,Cheng W.,Liu X.J.,and Liu X.X.,2016,Her-2 gene status and its correlation with expression of p53,ki-67,topo域in breast cancer,Xiandai Zhongliu Yixue(Journal of Modern Oncology),24(21):3382-3386(巩雷,陈萍,程薇,刘宪军,刘学鑫,2016,荧光原位杂交(fish)检测乳腺癌中her-2基因状态与p53、ki-67、topo域表达的相关性,现代肿瘤医学,24(21):3382-3386)
Guan J.Y.,2014,The physical locations of SUT and RZF gene families from Hevea brasiliensis,Thesis for M.S.,Hainan University,Supervisor:Zhuang N.S.,pp.21-45(官锦燕,2014,巴西橡胶树SUT和RZF基因家族物理定位的研究,硕士学位论文,海南大学,导师:庄南生,pp.21-45)
Guan J.Y.,Wang Y.,Gao H.Q.,and Zhuang N.S.,2014,Physical location of four HbRZF genes from rubber tree(Hevea brasiliensis),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(3):610-616(官锦燕,王英,高和琼,庄南生,2014,巴西橡胶树4个环锌指蛋白基因(HbRZF)的物理定位,基因组学与应用生物学,33(3):610-616)
Hall A.,and Lalli G.,2010,Rho and ras GTPases in axon growth,guidance,and branching,Cold Spring Harbor Perspectives in Biology,2(2):a001818
He D.X.,Li X.Y.,Wang J.J.,and Jiao K.,2015,Functions of small GTPase Rac1 in nodulation progress in Lotus japonicus,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),35(12):2365-2372(柯丹霞,李祥永,王静静,焦珂,2015,百脉根小G蛋白Rac1基因的克隆与功能分析,西北植物学报,35(12):2365-2372)
Huang Y.C.,2013,Gene cloning and expression analysis of the Rop gene family in Hevea brasiliensis,Thesis for M.S.,Hainan University,Supervisor:Tang C.Y.,pp.4-45(黄亚成,2013,橡胶树Rop小G蛋白基因家族的克隆与表达分析,硕士学位论文,海南大学,导师:唐朝荣,pp.4-45)
Jones M.A.,Shen J.J.,Fu Y.,Li H.,Yang Z.,and Grierson C.S.,2002,The Arabidopsis Rop2 GTPase is a positive regulator of both root hair initiation and tip growth,Plant Cell,14(4):763-776
Lang S.,Lei X.J.,Song J.,Yao L.N.,Hou Z.F.,Zheng H.Y.,Shang L.H.,and Wang Y.P.,2017,The applications of fluorescence in situ hybridization in studies on medicinal plants of Panax L.,Techan Yanjiu(Special Wild Economic Animal and Plant Research),39(2):63-66(梁韶,雷秀娟,宋娟,姚丽娜,侯志芳,郑惠元,商丽煌,王英平,2017,荧光原位杂交技术在人参属药用植物研究中的应用,特产研究,39(2):63-66)
Li X.Y.,Su L.L.,Gao J.J.,Wang Y.,Gao H.Q.,and Zhuang N.S.,2017,Physical location of six members of jaz gene family in Hevea brasiliensis,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(12):4992-4999(李晓燕,苏莉莉,高佳佳,王英,高和琼,庄南生,2017,JAZ基因家族6个成员在橡胶树上的物理定位,分子植物育种,15(12):4992-4999)
Li Y.,2016,Expression and functional analysis of PtROP and Pt Rab gene family in populus,Thesis for M.S.,Chinese Academy of Forestry,Supervisor:Lu M.Z.,pp.5-21(李煜,2016,杨树Pt ROP与Pt Rab基因家族表达分析及功能预测,硕士学位论文,中国林业科学研究院,导师:卢孟柱,pp.5-21)
Liu W.,Chen A.M.,Feng L.X.,Cao L.P.,Sun J.,and Wang Y.Z,2010,Role ofsmall GTP-binding protein ROP in M.truncatula in symbiosis:1.isolation and expression analysis of MtROP5 gene,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),43(7):1355-1362(刘伟,陈爱民,冯利兴,曹连莆,孙杰,王彦章,2010,蒺藜苜蓿小G蛋白ROP在共生过程中的作用:1.MtROP5的克隆和表达分析,中国农业科学,43(7):1355-1362)
Niu J.,2011,Preliminary study of banana small GTPases gene MaROP1 in transgenic Arabidopsis,Thesis for M.S.,Hainan University,Supervisor:Xu B.Y.,pp.4-16(牛杰,2011,香蕉小G蛋白基因MaROP1转化拟南芥的初步研究,硕士学位论文,海南大学,导师:徐碧玉,pp.4-16)
Song Y.C.,and Gustafson J.P.,1995,The physical location of fourteen RFLP markers in rice(Oryza sativa L.)Theor.Appl.Genet.,90(1):113-119
Sun P.G.,Miao H.X.,Xu B.Y.,and Jin Z.Q.,2013,Proceedings in biological function of small GTPases gene ROP of plant,Beifang Yuanyi(Northern Horticulture),(22):188-192(孙佩光,苗红霞,徐碧玉,金志强,2013,植物小G蛋白基因ROP生物学功能研究进展,北方园艺,(22):188-192)
Sundaravelpandian K.,Chandrika N.N.,and Schmidt W.,2013,PFT1,a transcriptional Mediator complex subunit,controls root hair differentiation through reactive oxygen species(ROS)distribution in Arabidopsis,New Phytol.,197(1):151-161
Tan R.F.,and Li L.,2006,Advances in research of ROP,Shengming Kexue Yanjiu(Life Science Research),10(2):80-84(谭汝芳,李玲,2006,小G蛋白ROP的研究进展,生命科学研究,10(2):80-84)
Tang C.R.,Yang M.,Fang Y.J.,Luo Y.F.,Gao S.H.,Xiao X.H.,An Z.W.,Zhou B.H.,Zhang B.,Tan X.Y.,Yeang H.Y.,Qin Y.X.,Yang J.,Lin Q.,Mei H.,Montoro P.,Long X.Y.,Qi J.,Hua Y.,He Z.,Sun M.,Li W.,Zeng X.,Cheng H.,Liu Y.,Yang J.,Tian W.,Zhuang N.,Zeng R.,Li D.,He P.,Li Z.,Zou Z.,Li S.,Li C.,Wang J.X.,Wei D.,Lai C.Q.,Luo W.,Yu J.,Hu S.N.,and Huang H.S.,2016,The rubber tree genome reveals new insights into rubber production and species adaptation,Nature Plants,2(6):1-10
Yang F.,2011,The establishment of patato suspension cell lines and the subcellular localization of small GTPase At Rop1,St Racl,Thesis for M.S.,Neimenggu University,Supervisor:Zhao J.,pp.8-30(杨帆,2011,马铃薯悬浮细胞系的建立及小G蛋白AtRop1,St Rac1的亚细胞定位,硕士学位论文,内蒙古农业大学,导师:赵君,pp.8-30)
Yang G.Y.,Zhen F.,Wang Y.,Gao H.Q.,and Zhuang N.S.,2018,Physical location of fish of five members in nac gene family from Hevea brasiliensis,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),16(2):512-517(杨光涌,郑菲,王英,高和琼,庄南生,2018,巴西橡胶树NAC基因家族5个成员的荧光原位杂交物理定位,分子植物育种,16(2):512-517)
Ying G.,Vernoud V.,Ying F.,and Yang Z.,2003,ROP GTPase regulation of pollen tube growth through the dynamics of tip-localized F-actin,J.Exp.Bot.,54(380):93-101
基本信息:
DOI：10.13417/j.gab.038.005534
中图分类号:S794.1
引用信息:
[1]陈秋惠,王英,高和琼,等.巴西橡胶树HbRop基因家族5个成员的染色体物理定位[J].基因组学与应用生物学,2019,38(12):5534-5541.DOI:10.13417/j.gab.038.005534.
基金信息:
国家自然科学基金项目(31360186);; 国家天然橡胶产业技术体系(CARS-34);; 海南大学热带作物学院研究生科研创新课题(ZWCX2018025)共同资助
2018-08-09
2018-08-09
2018-08-09