| 400 | 4 | 141 |
| 下载次数 | 被引频次 | 阅读次数 |
本研究对分别经过低浓度(5 mg/L)和高浓度(10 mg/L)恩诺沙星抗菌药物24 h短期浸泡处理后的东星斑(Plectropomus leopardus)抗氧化酶活力、抗氧化物质水平及消化酶活力进行了检测。实验结果表明:10mg/L恩诺沙星浸泡处理会显著影响东星斑抗氧化酶(超氧化物歧化酶SOD;过氧化氢酶CAT;过氧化物酶POD;溶菌酶LZM;谷胱甘肽过氧化物酶GSH-Px)正常活力的表达,并显著抑制抗氧化物质丙二醛(MDA)的表达;而5 mg/L恩诺沙星浸泡处理则不会。5 mg/L和10 mg/L恩诺沙星短期浸泡处理都不会对东星斑的消化酶(胃蛋白酶;脂肪酶;淀粉酶)的正常表达活性产生显著影响。因此,以低浓度恩诺沙星抗菌药物对东星斑进行短期浸泡处理以进行细菌性疾病的预防及治疗的方式不会对其本身酶活生理造成不可逆损伤,其中5 mg/L的浓度可作为该抗菌药物的安全浸泡浓度进行使用。
Abstract：In this study, Plectropomus leopardus antioxidant enzyme activity, antioxidant substance level and digestive enzyme activity were measured after short soak of enoxacin antibacterial drugs in low concentration(5 mg/L) and high concentration(10 mg/L) for 24 h. The results showed that 10 mg/L enrofloxacin solution affects the activity of SOD, CAT, POD, LZM, GSH-Px significantly and suppressed MDA level significantly, while 5 mg/L did not yet. The digestive enzymes activity were not affected by short-term immersion with 5 mg/L and 10 mg/L enrofloxacin bath administration. Thus, the antioxidant and digestive enzymes activity of Plectropomus leopardus were not affected significantly by short-term immersion treatment with low concentration enrofloxacin bath administration. And 5 mg/L enrofloxacin solution is suggested to use in Plectropomus leopardus aquaculturing as safe bath administration dose.
Deng J.M.,Mai K.S.,Ai Q.H.,Zhang W.B.,Tan B.P.,Xu W.,and Liufu Z.G.,2010,Alternative protein sources in diets for Japanese flounder Paralichthys olivaceus(Temminck and Schlegel):Ⅱ.effects on nutrient digestibility and digestive enzyme activity,Aquaculture Res.,41(6):861-870
Dong L.,He Y.Z.,Wang Y.L.,and Dong Z.Y.,2013,Research progress on application of superoxide dismutase(SOD),Zhongguo Nongye Keji Daobao(Journal of Agricultural Science and Technology),15(5):53-58(董亮,何永志,王远亮,董志扬,2013,超氧化物歧化酶(SOD)的应用研究进展,中国农业科技导报,15(5):53-58)
Fang X.,Liu X.,Liu W.,and Lu C.,2012,Pharmacokinetics of enrofloxacin in allogynogenetic silver crucian carp,Carassius auratus gibelio,J.Vet.Pharmacol.Ther.,35(4):397-401
Gu L.B.,Xu L.W.,Feng J.,Su Y.L.,Liu G.F.,and Guo Z.X.,2015,Identification and drug sensitive test of bacterial pathogens from Plectropomus leopardus with tail fester disease,Nanfang Shuichan Kexue(South China Fisheries Science),11(4):71-80(辜良斌,徐文力,冯娟,苏友禄,刘广峰,郭志勋,2015,豹纹鳃棘鲈尾部溃烂症病原菌的鉴定与药敏试验,南方水产科学,11(4):71-80)
Jiang X.L.,Li Z.Q.,and Kang Z.S.,2001,The recent progress of research on peroxidase in plant disease resistance,Xibei Nonglin Keji Daxue Xuebao(Ziran Kexue Ban)(Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition)),29(6):124-129(蒋选利,李振岐,康振生,2001,过氧化物酶与植物抗病性研究进展,西北农林科技大学学报(自然科学版),29(6):124-129)
Li M.,Yu N.,Qin J.G.,Li E.C.,Du Z.Y.,and Chen L.Q.,2014,Effects of ammonia stress,dietary linseed oil and Edwardsiella ictaluri challenge on juvenile darkbarbel catfish Pelteobagrus vachelli,Fish Shellfish Immunol.,38(1):158-165
Liu T.M.,Wang Y.B.,Fu S.Y.,Yang M.Q.,Fu Y.F.,and Li X.M.,2012,On the artifical seedling of Plectropomus leopardus in large outdoor cement pools,Haiyang Yuye(Marine Fisheries),34(4):400-495(刘天密,王永波,符书源,杨明秋,符一凡,李向民,2012,豹纹鳃棘鲈室外大型水泥池的人工育苗技术,海洋渔业,34(4):400-405)
Liu Y.J.,Hu J.,Zhou S.J.,Yang R.,Qin J.G.,Ma Z.H.,and Yang Q.B.,2018,Effect of acute ammonia stress on antioxidant enzymes and digestive enzymes in barramundi Lates calcarifer Larvae,Isr.J.Aquacult.Bamid.,15(8):1-6
Ma Z.H.,Zhang N.,Guo H.Y.,Zheng P.L.,and Zhang D.C.,2015,Replacement of frozen fish meat based diet with artificial diets in rearing of coral trout Plectropomus leopardus(Lacepede,1802)fingerlings,Indian J.Fish.,62(2):118-122
Meng Q.W.,2006,Studies on feeding behavior and digestive enzyme activities of Litopenaeus vannamei and Fenneropenaeus chinensis in different culture conditions,Thesis for M.S.,Fisheries College of Ocean University of China,Supervisor:Zhang X.M.,pp.1-5(孟庆武,2006,不同养殖模式中对虾摄食行为及消化酶活力的研究,硕士学位论文,中国海洋大学水产学院,导师:张秀梅,pp.1-5)
Ren X.Y.,2014,Study on toxicity assessment of polycyclic aromatic hydrocarbons and antibiotics on Litopenaeus vannamei,Dissertation for Ph.D.,Fisheries College of Ocean University of China,Supervisor:Pan L.Q.,pp.16-21(任宪云,2014,多环芳烃和渔用抗生素在凡纳滨对虾体内蓄积毒性效应的研究,博士学位论文,中国海洋大学水产学院,导师:潘鲁青,pp.16-21)
Sun L.S.,and GAO Q.,2006,Biotechnological applications of peroxidasess,Huagong Jishu yu Kaifa(Technology&Devel opent of Chemical Industry),35(12):13-16,28(孙立水,高强,2006,过氧化物酶的应用研究进展,化工技术与开发,35(12):13-16,28
Sun Y.C.,Wang R.X.,Zhao M.M.,Cao C.,Lu Y.S.,Yao T.,and Wang J.Y.,2017,Study of antibiotic-resistance of heterotrophic bacteria from intestines of abalone(Haliotis discus hannai)and farming water,Nanfang Shuichan Kexue(South China Fisheries Science),13(3):58-65(孙永婵,王瑞旋,赵曼曼,曹超,鲁义善,姚托,王江勇,2017,鲍消化道及其养殖水体异养菌的耐药性研究,南方水产科学,13(3):58-65)
Sun Z.J.,Jiang S.G.,Yu Y.G.,Zhang Z.K.,and Xia S.D.,2013,Effects of temperature on the feeding,growth and survival of the juvenile Plectropomus leopardus,Hebei Yuye(Hebei fisheries),(5):1-4,12(孙志景,江曙光,于燕光,张振奎,夏苏东,2013,水温对东星斑幼鱼存活、摄食及生长的影响,河北渔业,(5):1-4,12)
Yang F.,and Sun H.C.,2010,Effects of enrofloxacin on activities of CAT in Carassius auratus,Hubei Nongye Kexue(Hubei Agricultural Sciences),49(1):156-158(杨帆,孙翰昌,2010,恩诺沙星对鲫鱼过氧化氢酶活性的影响,湖北农业科学,49(1):156-158)
Yang J.D.,Wei F.J.,Pan X.X.,Lv Z.P.,Tang S.J.,and Zhang Y.J.,2016,Research process of animal catalase(CAT),Heilongjiang Xumu Shouyi(Heilongjiang Animal Science and Veterinary Medicine),(7):59-62(杨佳栋,魏凤菊,潘新新,吕转萍,唐思静,张英杰,2016,动物过氧化氢酶(CAT)的研究进展,黑龙江畜牧兽医,(7):59-62)
Yang Y.K.,Yu W.,Lin H.Z.,Li T.,Huang X.L.,Huang Z.,and Qi C.L.,2017,Experimental starvation of Plectropomus leopardus larvae and study of point of no return,Nanfang Shuichan Kexue(South China Fisheries Science),13(6):90-96(杨育凯,虞为,林黑着,李涛,黄小林,黄忠,戚常乐,2017,豹纹鳃棘鲈仔鱼饥饿实验和不可逆点研究,南方水产科学,13(6):90-96)
Yoseda K.,Yamamoto K.,Asami K.,Chimura M.,Hashimoto K.,and Kosaka S.,2008,Influence of light intensity on feeding,growth,and early survival of leopard coral grouper(Plectropomus leopardus)larvae under mass-scale rearing conditions,Aquaculture,279(1-4):55-62
Zhang B.,Meng Z.H.,Liu B.S.,Li H.M.,Su J.Q.,Huang G.J.,Wu K.C.,and Yu D.H.,2017,Effect of nucleus-inserting surgery damage on anti-oxidation and immunity of pearl oyster(Pinctada fucata),Nanfang Shuichan Kexue(South China Fisheries Science),13(5):72-77(张博,孟子豪,刘宝锁,李海梅,苏家齐,黄桂菊,吴开畅,喻达辉,2017,插核手术损伤对合浦珠母贝抗氧化免疫水平的影响,南方水产科学,13(5):72-77)
Zhang L.M.,Wu Z.H.,Jian J.C.,Lu Y.S.,and Jian H.J.,2007,Effects of florfenicol on immune indexes of crimson snapper(Lutjanus sanguineus),Guangdong Haiyang Daxue Xuebao(Journal of Guangdong Ocean U niversity)27(3):50-54(张丽敏,吴灶和,简纪常,鲁义善,简贺君,2007,注射氟苯尼考对红笛鲷免疫指标的影响,广东海洋大学学报,27(3):50-54)
Zhang L.M.,Wu Z.H.,Jian J.C.,Lu Y.S.,and Jian H.J.,2009,Effects of enrofloxacin on AKP activity,Ig M content,lysozyme content and antibiotic ability of Crimson snapper(Lutjanus sanguineus),Shuichan Yangzhi(Journal of Aquaculture),30(4):1-7(张丽敏,吴灶和,简纪常,鲁义善,简贺君,2009,恩诺沙星对红笛鲷血清中碱性磷酸酶活力、抗体Ig M含量、溶菌酶含量及其抗菌能力的影响,水产养殖,30(4):1-7)
Zhang Z.,Li J.,Feng W.,He Y.Y.,and Chen P.,2011,Effects of norfloxacin on the non-specific immune response of Fenneropenaeus chinensis,Yuye Kexue Jinzhan(Progress in Fishery Sciences),32(2):53-59(张喆,李健,冯伟,何玉英,陈萍,2011,不同浓度诺氟沙星对中国对虾非特异性免疫酶活的影响,渔业科学进展,32(2):53-59)
基本信息:
DOI：10.13417/j.gab.039.003977
中图分类号:S948
引用信息:
[1]吝思琪,胡静,周胜杰,等.抗菌药物短期浸泡对东星斑抗氧化酶、物质及消化酶活性的影响[J].基因组学与应用生物学,2020,39(09):3977-3982.DOI:10.13417/j.gab.039.003977.
基金信息:
广西创新驱动发展专项资金项目(桂科AA18242031);; 现代农业产业技术体系建设项目(CARS-47)共同资助
2020-09-25
2020-09-25