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由布鲁氏菌所引起的布鲁氏菌病,主要特征为发热、流产,是世界范围之内人畜共患慢性细菌传染病,对多种动物的健康带来了严重的影响,同时也影响着人们的生命健康和生产生活。布鲁氏菌能够感染多种家畜、野生动物,也可以感染人类,给国家、养殖户以及生产者带来了严重的损失,还会引起一系列公共卫生的问题。基于此,研究布鲁氏菌检查办法,能够为该病的预防和治疗提供针对性的解决信息。本研究主要阐述了免疫学ELASA和PCR等布鲁氏菌检测技术,这些快速检测技术均能够准确的检测出布鲁氏菌。
Abstract：Brucella disease is caused by Brucella and characterized by fever and abortion. It is a zoonotic and chronic bacterial infectious disease in the world, which has brought serious influence on the health of a variety of animals, and also affects the health and production and living of people. Brucella can infect a variety of livestock,wild animals and also humans, which can cause serious losses to the country, farmers and producers, and also brings about a series of problems in public health. Based on this, the study on the inspection methods of Brucella can provide targeted information for the prevention and treatment of disease. This research mainly expounded the ELISA and PCR immunological detection technology of Brucella, which are able to accurately detect Brucella.
Chen S.T.,2013,Cloning,expression and antigenicity identifica tion of Brucella abortus and Mycobacterium bovis antigen,Dissertation for Ph.D.,Jinan University,Supervisor:Tang S.Q.,pp.3-14(陈诗涛,2013,牛布鲁氏菌和牛分枝杆菌抗原的基因克隆、表达和抗原性鉴定,博士学位论文,暨南大学,导师:汤顺清,pp.13-21)
Chen T.T.,Shi F.,Chen C.F.,Wang Y.Z.,Zhang H.,Li Z.Q.,Lu Z.Z.,Jian T.,and Ma Q.M.,2014,Establishment of brucella antibody colloidal gold immunochromatography assay for rapid detection technology,Zhongguo Bingyuanshengwuxue Zazhi(Journal of Pathogen Biology),7(2):122-126(程婷婷,石峰,陈创夫,王远志,张辉,李志强,鲁芝子,监通,马齐蔓,2014,布鲁氏菌抗体胶体金免疫层析法快速检测技术的建立,中国病原生物学杂志,7(2):122-126)
Cheng T.T.,2014,Development of the colloldal gold Immunochromatographic Strip for Diagnosis of Brucella serum and Lyme disease,Thesis for Ph.D.,Shihezi University,Supervisor:Chen C.F.,pp.10(程婷婷,2014,布鲁氏菌病与莱姆病胶体金免疫层析试纸条的研制,硕士学位论文,石河子大学,导师:陈创夫,pp.10)
Li J.Y.,2012,A method for rapid detection of Brucella,Dissertation for Ph.D.,Inner Mongolia Agricultural University,Supervisor:Li.Y.Z.,pp.11-16(李建云,2012,动物布鲁氏菌快速检测方法的研究,博士学位论文,内蒙古农业大学,导师:李云章,pp.11-16)
Lin G.Z.,2012,Establishment of brucellosis lamp detection method and double gene co expression of molecular vaccine research,Dissertation for Ph.D.,Chinese Academy of Agri cultural Sciences,Supervisor:Qiu C.Q.,pp.3-14(蔺国珍,2012,布鲁氏菌病LAMP检测方法的建立及双基因共表达分子疫苗研究,博士学位论文,中国农业科学院,导师:邱昌庆,pp.3-14)
Song L.Y.,and Chen S.Y.,2010,Research progress on rapid detection of Brucella,Redai Yixue Zazhi(Journal of Tropical Medicine),10(11):1361-1363(宋榴艳,陈守义,2010,布鲁氏菌快速检测技术研究进展,热带医学杂志,10(11):1361-1363)
基本信息:
DOI：10.13417/j.gab.036.000693
中图分类号:S852.61
引用信息:
[1]李向阳,霍晓伟,武迎红,等.牛布鲁氏菌病原快速检测技术研究[J].基因组学与应用生物学,2017,36(02):693-695.DOI:10.13417/j.gab.036.000693.
基金信息:
国家自然科学基金项目(31260608);; 内蒙古自治区高等学校科学技术研究重点项目(NJZZ12117);; 内蒙古通辽市校科技合作项目(SXZD2012131);; 内蒙古自治区自然科学基金项目(2015MS0339)共同资助
2017-02-25
2017-02-25