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本研究以三角梅(Bougainvillea glabra)品系‘Bouform 1’种子下胚轴为外植体，通过研究在MS培养基上添加不同的植物生长调节剂，其在诱导分化以及不定芽增殖、壮苗、生根等阶段的生长状况，以期筛选出最适宜其生长的培养基，建立稳定有效的再生体系。结果表明：下胚轴诱导分化最佳培养基为MS+IBA(吲哚丁酸)0.1 mg/L+6-BA(6-苄氨基嘌呤) 2.0 mg/L，分化率和平均分化芽数为81.35%和7.04个；不定芽增殖最佳培养基为MS+NAA(萘乙酸) 0.05 mg/L+6-BA 3.0 mg/L，增殖系数达到5.22；壮苗最佳培养基为MS+NAA 0.1 mg/L+6-BA 1.0 mg/L，平均苗高达到5.59 cm；不定芽生根最佳培养基为1/2 MS+NAA 0.2 mg/L，生根率和平均生根数最高，分别为82.54%和4.38条。本研究建立的三角梅下胚轴再生技术，为后续离体突变体诱导、多倍体育种及遗传转化体系的建立提供了技术保障。
Abstract：To establish a stable and effective in vitro regeneration protocol, in the present investigation, the seed hypocotyl of Bougainvillea glabra‘Bouform 1'was used as the explant, and the growth status of induced differentiation, adventitious buds proliferation, strong seedling, rooting and so on was evaluated on MS medium supplemented with various plant growth regulators. The results showed that the optimal medium for adventitious differentiation was MS+IBA 0.1 mg/L+6-BA 2.0 mg/L, with the differentiation rate at 81.35% and the differentiated buds at 7.04 on average. The medium for adventitious buds proliferation was MS+NAA 0.05 mg/L+6-BA 3.0 mg/L, with the proliferation coefficient at 5.22. The seedling strength medium was MS+NAA 0.1 mg/L+6-BA 1.0 mg/L and the average seedling height reached 5.59 cm. The rooting medium was 1/2 MS+NAA 0.2 mg/L, with the rooting rate at 82.54% and 4.38 roots per seedling on average. The Bougainvillea hypocotyl regeneration technology established in this study provides technical support for subsequent in vitro mutant induction, polyploidy breeding and the establishment of genetic transformation systems.
Bu X.X.,and Chen W.L.,1988,Adsorption of activated carbon on plant growth regulators in culture medium,Zhiwu Shengli Xuebao(Plant Physiology Journal),14(4):401-405.(卜学贤,陈维伦,1988,活性炭对培养基中植物生长调节物质的吸附作用,植物生理学报,14(4):401-405.)
Du T.K.,and Xu Q.,2004,Tissue culture of Bougainvillea spectabilis,Ningxia Daxue Xuebao(Journal of Ningxia University(Natural Science)),(1):69-71.(杜天奎,徐青,2004,叶子花的组织培养,宁夏大学学报(自然科学版),(1):69-71.)
Gao H.B.,Tang X.J.,and Meng Q.F.,2006,Effect of high level of 6-BA on the endogenous hormones level changes in the vitrification seeding of Prunus cerasus,Linye Kexue Yanjiu(Forest Research),(4):488-490.(高红兵,唐晓杰,孟庆繁,2006,高浓度6-BA诱导酸樱桃苗的玻璃化苗内源激素含量变化,林业科学研究,(4):488-490.)
Javed A.M.,Said H.,and Saima N.,1996,In vitro propagation of(Bougainvillea spectabilis) through shoot apex culture,Pak.J.Bot.,28(2):207-211.
Liu F.,Zhu W.L.,Huang M.Z.,Ding Z.T.,and Tang Y.Q.,2017,Inducement of philippine ground orchid(Spathoglottis plicata) protocormlike bodies and plant regeneration,Jiyinzuxue yu Yingyong Shengwuxue(Genomics and Applied Biology),36(5):2082-2087.(刘帆,朱文丽,黄明忠,丁泽婷,唐燕琼,2017,紫花苞舌兰类原球茎诱导及植株再生,基因组学与应用生物学,36(5):2082-2087.)
Liu X.L.,Li B.P.,Liu L.P.,and Fan X.F.,2017,Callus induction and plant regeneration of Bougainvillea glabra Choisy,Linye Keji Tongxun(Forest Science and Technology),(1):25-30.(刘秀丽,李博萍,刘丽萍,范小峰,2017,三角梅愈伤组织诱导和植株再生,林业科技通讯,(1):25-30.)
Li Z.Y.,2017,Comparation on culture propogation of three varieties of Bougainvillea spectabilis,Thesis for M.S.,Guangxi University,Supervisor:Wen Y.G.,pp.14-32.(李祖毅,2017,三种三角梅品种的组培繁殖比较研究,硕士学位论文,广西大学,导师:温远光,pp.14-32.)
Lu Z.,Huang C.M.,Wei Y.W.,Deng Z.N.,Cao H.Q.,Wu K.C.,Luo H.B.,Jiang S.L.,and Xu L.,2019,Study on dedifferentiation regeneration system of Passiflora edulis,Zhongguo Guoshu(China Fruits),(6):65-68.(陆珍,黄诚梅,魏源文,邓智年,曹辉庆,吴凯朝,罗海斌,蒋胜理,徐林,2019,西番莲脱分化再生体系研究,中国果树,(6):65-68.)
Ma L.X.,Wang S.Y.,Wang Z.,and Zhou Y.,2018,Establishment of tissue culture regeneration system of Flax variety neiya no.7,Zhongzi(Seed),37(12):73-76.(马立晓,王树彦,王卓,周宇,2018,胡麻品种内亚7号下胚轴组织培养再生体系的建立,种子,37(12):73-76.)
Mu Y.,Hong J.Y.,Chen J.,Tang C.G.,Ni J.,Chen M.,and Wu L.F.,2016,Establishment of direct regeneration system from hypocotyls of tung tree(Vernicia fordii),Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(7):1821-1826.(穆燕,侯金艳,陈晶,汤才国,倪军,陈明,吴丽芳,2016,油桐下胚轴直接再生体系的建立,分子植物育种,14(7):1821-1826.)
Pang L.,Lin S.Z.,Cao G.Q.,and Xiao X.P.,2008,A study on induction root of tissue-cultured seedling on Chinese fir superior clones,Jiangxi Nongye Daxue Xuebao(Acta Agriculturae Uniersitatis Jiangxiensis),30(4):283-286.(庞丽,林思祖,曹光球,肖晓蓬,2008,杉木优良无性系组培苗诱导根的研究,江西农业大学学报,30(4):283-286.)
Sharma A.K.,Prasad R.N.,and Chaturvedi H.C.,1981,Clonal propagation of Bougainvillea glabra‘Magnifica’through shoot apex culture,Plant Cell Tissue Organ Cult.,(1):33-38.
Wang Z.W.,Zhang Y.,Qi S.Z.,Zhu J.J.,Yuan W.,Zhang Y.D.,Liu X.Z.,and Zhang J.F.,2018,Tissue culture regeneration of hybrid Liquidambar styraciflua×L.formosana,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),40(8):42-49.(王泽伟,张炎,齐帅征,朱军杰,袁玮,张亚东,刘学增,张金凤,2018,杂种枫香组织培养再生研究,北京林业大学学报,40(8):42-49.)
Ye D.Y.,and Zhang J.,2004,Study on technique system of the tissue culture in Bougainvillea glabra,Sichuan Nongye Daxue Xuebao(Sichuan Agricultural University),22(2):142-145.(叶顶英,张建,2004,三角梅组织培养外植体再生体系建立研究,四川农业大学学报,22(2):142-145.)
Ye D.Y.,2011,Study on the in vitro and ex vitro rooting for test-tube seedlings of Bougainvillea glabra,Beifang Yuanyi(Northern Horticulture),(15):169-171.(叶顶英,2011,三角梅组培苗试管内外生根研究,北方园艺,(15):169-171.)
Zhu L.H.,and Wu X.Q.,2005,Factor affecting root formation of slash pine shoots by tissue culture,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),(5):15-18.(朱丽华，吴小芹，2005，湿地松组培苗生根的影响因子,东北林业大学学报,(5):15-18.)
Zhao T.,2019,Development of SSR molecular marker of transcriptome and construction of vitro regeneration system in Bougainvillea,Thesis for M.S.,Hainan University,Supervisor:Yin J.M.,pp.35-43.(赵彤,2019,三角梅转录组SSR分子标记开发与离体再生体系构建,硕士学位论文,海南大学,导师:尹俊梅,pp.35-43.)
基本信息:
DOI：10.13417/j.gab.040.003655
中图分类号:S685.99
引用信息:
[1]陆锦萍,冷青云,赵彤,等.三角梅下胚轴再生体系的建立[J].基因组学与应用生物学,2021,40(Z4):3655-3662.DOI:10.13417/j.gab.040.003655.
基金信息:
中国热带农业科学院基本科研业务费专项资金项目(1630032017020; 1630032017022); 海南省重大科技计划项目(zdkj201815)共同资助
2021-04-30
2021-04-30
2021-04-30