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CCAAT增强子结合蛋白(C/EBPs)家族是具有碱性一亮氨酸拉链结构的一类蛋白家族。为对普通牛CCAAT增强子结合蛋白(C/EBPs)家族基因编码蛋白的结构和功能进行生物信息学探析，本研究运用多个生物信息学分析软件分别对普通牛C/EBPs蛋白的理化性质、亲疏水性、基因结构、保守基序、亚细胞定位、染色体定位等进行了具体分析，构建了不同物种C/EBPs基因家族编码蛋白的系统发育树，并比对多种软件寻找准确的预测工具。通过分析研究发现，普通牛C/EBPs基因家族不同基因所编码蛋白质为亲水性不稳定蛋白，无跨膜结构和信号肽，均为非分泌型蛋白，家族中6个成员都是丝氨酸(Ser)磷酸化位点占比最大；但是普通牛C/EBPs基因家族不同基因所编码的氨基酸数、理论等电点、二级结构、染色体定位、保守基序等却存在一定差异；普通牛C/EBPs基因与牦牛、水牛、瘤牛亲缘关系较近，与鸡的遗传距离最远。这些研究结果为后续对牛C/EBPs家族基因功能的深入研究提供了重要的科学依据和参考，为其他使用软件对基因进行生物信息学分析的研究者提供了借鉴。
Abstract：The CCAAT enhancer binding protein(C/EBPs) family is a family of proteins with basic-leucine zipper structure. In order to analyze the structure and function of proteins encoded by common bovine CCAAT enhancer binding protein(C/EBPs)family genes, several bioinformatics analysis software were used to analyze the physical and chemical properties, hydrophilicity, gene structure, conserved motif, subcellular localization and chromosome location of common bovine C/EBPs protein. The phylogenetic trees of proteins encoded by C/EBPs gene family of different species were constructed, and the accurate prediction tools were compared with many kinds of software.Through analysis and study, it was found that the proteins encoded by different genes of the common bovine C/EBPs gene family were hydrophilic unstable proteins without transmembrane structure and signal peptide, all of them were non-secretory proteins, and the serine(Ser) phosphorylation sites accounted for the largest proportion of the six members of the family. However, there are some differences in the number of amino acids, theoretical isoelectric point, secondary structure, chromosome location and conserved motif encoded by different genes of the common bovine C/EBPs gene family, and the common bovine C/EBPs gene is closely related to consumption cattle, buffalo and tumor buffalo, and has the farthest genetic distance from chicken. These results provide an important scientific basis and reference for the further study of the gene function of bovine C/EBPs family, and provide a reference for other researchers who use software to analyze genes in bioinformatics.
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基本信息:
DOI：10.13417/j.gab.040.002944
中图分类号:S823
引用信息:
[1]周子航,张娟,封元,等.普通牛CCAAT增强子结合蛋白(C/EBPs)家族基因的生物信息学分析[J].基因组学与应用生物学,2021,40(Z3):2944-2958.DOI:10.13417/j.gab.040.002944.
基金信息:
宁夏回族自治区畜牧工作站的宁夏安格斯牛核心群建设项目; 宁夏自然科学基金项目(2019AAC03011)共同资助
2020-11-17
2020-11-17
2020-11-17