| 195 | 1 | 28 |
| 下载次数 | 被引频次 | 阅读次数 |
为探讨MDR1基因沉默对姜黄素诱导人胃癌SGC7901/ADM细胞凋亡的影响,将已构建的靶向MDR1基因的RNAi表达载体转染SGC7901/ADM细胞,建立转染细胞单克隆,流式细胞术检测细胞外排功能。姜黄素处理SGC7901/ADM细胞48 h后,通过激光共聚焦显微镜下观察细胞形态结构,琼脂糖凝胶电泳检测DNA片段化。结果显示,各干扰载体转染组细胞内Rho-123荧光强度不同程度增强,细胞经姜黄素处理48 h后,激光共聚焦显微镜下呈明显的凋亡形态结构,DNA琼脂糖凝胶电泳呈梯状条带,说明MDR1基因沉默能促进姜黄素诱导SGC7901/ADM细胞凋亡。
Abstract：The aim of this study was to investigate the effects of MDR1 gene silencing on the apoptosis of human gastric cancer SGC7901/ADM cells induced by curcumin. The constructed RNAi expression vector of targeting MDR1 gene was transfected into SGC7901/ADM cells, and one single clone was established, the cell efflux function was analyzed by flow cytometry. After the cells were treated with curcumin 48 hours, the morphological of the cells was observed under a confocal laser scanning microscope. The DNA fragmentation of the cells was evaluated by agarose gel electrophoresis. The results showed that fluorescence intensity of Rho-123 in the cells was transfected by various interference carriers was enhanced in different degrees. After treated with curcumin for 48 h, the apoptotic morphological structure was observed under laser confocal microscope, and DNA agarose gel electrophoresis showed a ladder band, which indicated that MDR1 gene silencing could promote the apoptosis of SGC7901/ADM induced by Curcumin.
Amin A.R.,Haque A.,Rahman M.A.,Chen Z.G.,Khuri F.R.,and Shin,D.M.,2015,Curcumin induces apoptosis of upper aerodigestive tract cancer cells by targeting multiple pathways,PLos One,10(4):e0124218
Cho S.,Lu M.,He X.L.,Ee P.L.,Bhat U.,Schneider E.,Miele L.,and Beck W.T.,2011,Notch1 regulates the expression of the multidrug resistance gene ABCC1/MRP1 in cultured cancer cells,Proc.Natl.Acad.Sci USA,108(51):20778-20783
Choi Y.K.,Cho S.G.,Choi H.S.,Woo S.M.,Yun Y.J.,Shin Y.C.,and Ko S.G.,2013,Jnk1/2 activation by an extract from the roots of morus alba l.reduces the viability of multidrug-resistant mcf-7/dox cells by inhibiting yb-1-dependent mdr1expression,Evidence-based complementary and alternative medicine,2013(6):741985
Ge L.,Wang H.J.,Yin D.,Lei C.,Zhu J.F.,Cai X.H.,and Zhang G.Q.,2012,Effectiveness of 5-flurouracil-based neoadjuvant chemotherapy in locally-advanced gastric/gastroesophageal cancer:A meta-analysis,World Journal of Gastroenterology,18(48):7384-7393
Kim S.G.,Ji S.M.,Lee N.R.,Park S.H.,You J.H.,Choi I.J.,Lee W.S.,Park S.J.,Lee J.H.,Seol S.Y.,Kim J.H.,Lim C.H.,Cho J.Y.,Kim G.H.,Chun H.J.,Lee Y.C.,Jung H.Y.,and Kim J.J.,2016,Quality of life after endoscopic submucosal dissection for early gastric cancer:a prospective multicenter cohort study,Gut&Liver,11(1):87-92
Litviakov N.V.,Cherdyntseva N.V.,Tsyganov M.M.,Slonimskaya E.M.,Ibragimova M.K.,Kazantseva P.V.,Kzhyshkowska J.,and Choinzonov E.L.,2016,Deletions of multidrug resistance gene loci in breast cancer leads to the down-regulation of its expression and predict tumor response to neoadjuvant chemotherapy,Oncotarget,7(7):7829-7841
Mansoori M.,Golalipour M.,Alizadeh S.,Jahangirerad A.,Khandozi S.R.,Fakharai H.,and Shahbazi M.,2014,Genetic variation in the ABCB1 gene may lead to m RNA level chabge:application to gastric cancer cases,Asian Pac.J.Cancer Prev.,16(18):8467-8471
Shao S.L.,Li X.Y.,Zhang W.W.,Yun D.Z.,Fu B.,and Zhang Z.Z.,2012,Effect of RNAi silencing MDR1 gene on the sensitivity of multidru g resistant actue promyelocytic leukemia cells HT9 to drugs,Zhongguo Xibao Shengwu Xuebao(Chinese Journal of Cell Biology),(10):983-987(邵淑丽,李旭艳,张伟伟,恽东泽,付博,张珍珠,2012,沉默MDR1基因增强急性早幼粒白血病耐药细胞HT9药物敏感性,中国细胞生物学学报,(10):983-987)
Strong V.E.,Wu A.,Selby L.V.,Gonen M.,Hsu M.,Song K.Y.,Park C.H.,Coit D.G.,Ji J.F.,and Brennan M.F.,2015,Differences in gastric cancer survival between the U.S.and china,Journal of Surgical Oncology,112(1):31-37
Sun X.D.,Shao.S.L.,Yun D.Z.,Li X.Y.,Zhang W.W.,Fu B.,and Li Y.,2017,Silencing MDR1 gene enhances the sensibility of SGC7901/ADM cells to curcumin,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(6):2486-2490(孙新迪,邵淑丽,恽冬泽,李旭艳,张伟伟,付博,李妍,2017,沉默MDR1基因增强SGC7901/ADM对姜黄素的敏感性,基因组学与应用生物学,36(6):2486-2490)
Sun X.R.,Sun J.J.,and Zhang L.X.,2015,Research progress on the mechanism of multidrug resistance of tumor,Xiandai Zhongliu Yixue(Journal of Modern Oncology(Modern Oncology)),25(1):164-166(孙晓冉,孙剑经,张林西,2015,肿瘤多药耐药机制的研究进展,现代肿瘤医学,25(1):164-166)
Waghela B.N.,Sharma A.,Dhumale S.,Pandey S.M.,and Pathak C.,2015,Curcumin conjugated with plga potentiates sustainability,anti-proliferative activity and apoptosis in human colon carcinoma cells,PLo S One,10(2):e0117526
Wang W.L.,Ji Y.H.,Wan L.X.,and Qu Z.Y.,2015,Effect of curcumin on proliferation and apoptosis of human hepatocellular carcinoma cell line snu475,Zhongyao Yaoli Yu Linchuang(Pharmacology and Clinics of Chinese Materia Medica),31(4):47-50(王文廉,姬颖华,万立新,屈中玉,2015,姜黄素对人肝癌细胞snu475增殖及凋亡的影响,中药药理与临床,31(4):47-50)
Watson J.L.,Hill R.,Yaffe P.B.,Greenshields A.,Walsh M.,Lee,P.W.,Giacomantonio C.A.,and Hoskin D.W.,2010,Curcumin causes superoxide anion production and p53-independen apoptosis in human colon cancer cells,Cancer letters,297(1):1-8
Ye Q.,Wang R.P.,and Zou X.,2016,Research progress of Chinese medicine reversing multidrug resistance of gastric cancer,Zhonghua Zhongyiyao Zazhi(China Journal of Traditional Chinese Medicine and Pharmacy),2016(8):3194-3197(叶青,王瑞平,邹玺,2016,中药逆转胃癌多药耐药作用的研究进展简,中华中医药杂志,2016(8):3194-3197)
Zhang W.W.,Shao S.L.,Zhang Z.Z.,Zhang Y.,and Fu B.,2013,Silencing MDR1 gene expression reverses resistance of drug-resistant leukemia HT9 cells to allicin,Zhongguo Zhongliu Shengwu Zhiliao Zazhi(Chinese Journal of Cancer Biotherapy),20(1):52-57(张伟伟,邵淑丽,张珍珠,张宇,付博,2013,沉默MDR1基因表达逆转白血病耐药HT9细胞对大蒜素的耐药性,中国肿瘤生物治疗杂志20(1):52-57)
Zhao J.J.,Wang D.,Yao H.,Sun D.W.,and Li H.Y.,2015,Ctla-4and mdr1 polymorphisms increase the risk for ulcerative colitis:a meta-analysis,World Journal of Gastroenterology,21(34):10025-10040
基本信息:
DOI：10.13417/j.gab.037.003066
中图分类号:R285.5
引用信息:
[1]孙新迪,邵淑丽,李旭艳,等.姜黄素诱导沉默MDR1基因的SGC7901/ADM细胞凋亡[J].基因组学与应用生物学,2018,37(07):3066-3070.DOI:10.13417/j.gab.037.003066.
基金信息:
黑龙江省自然科学基金(No.C201241;No.C200624;No.C2016057);; 黑龙江省教育厅科学技术项目(12511611);黑龙江省教育厅基本业务专项理工重点项目(135109104);; 齐齐哈尔大学研究生创新科研项目(YJSCX2016-ZD09)共同资助
2018-06-22
2018-06-22
2018-06-22