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为了获得α-LA作用肺癌细胞后的转录组数据库和差异表达基因,我们将A549细胞处理组和对照组作为测试样品,采用Illumina Hi Seq TM2000测序技术进行转录组测序,并进行系统的生物信息学分析。对照组和处理组两两比较共获得6 748个差异表达基因。GO(gene ontology)分类分析表明,差异表达基因属于细胞组分、分子功能和生物学过程的46个类别。KEGG Pathway显著性富集分析提示差异表达基因共涉及15条途径,包括肿瘤相关通路、内质网蛋白加工、细胞周期、核糖体、剪接体等相关途径。对α-LA作用肺癌细胞的转录组进行拼接、组装和功能注释,得到大量转录本信息,为探究α-LA作用肺癌细胞后基因差异表达及相关分子机制提供了宝贵的基因组数据库资源。
Abstract：To obtain the transcriptome database and differentially expressed genes of lung cancer cells treated with α-LA, We took the treated group and control group of A549 cells as experimental samples. Illumina Hi Seq TM2000 sequencing technology was used for transcriptome sequencing and systematic bioinformatics analysis. A total of 6 748 differentially expressed genes were obtained after pairwise comparisons on the control and treated groups. GO(gene ontology) classification analysis showed that differentially expressed genes belonged to 46 categories of cellular components, molecular function and biological processes. KEGG Pathway significant enrichment analysis indicated that differentially expressed genes involved 15 pathways, including pathways related with tumor, protein processing in endoplasmic reticulum, cell cycle, ribosome, spliceosome, etc. The transcriptome of lung cancer cells treated with α-LA was spliced, assembled and annotated to obtain a large number of transcript information, which might provide valuable genomic database resources for exploring the differential expression of gene and the related molecular mechanism of lung cancer cells treated with α-LA.
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基本信息:
DOI：10.13417/j.gab.037.001117
中图分类号:R734.2
引用信息:
[1]文娅,张满桥,陆菁潇,等.α-硫辛酸作用肺癌细胞的转录组分析[J].基因组学与应用生物学,2018,37(03):1117-1123.DOI:10.13417/j.gab.037.001117.
基金信息:
国家自然科学基金面上项目:Grb2介导α-硫辛酸抑制肺癌细胞增殖的机制研究(编号:81672282)、ILK在纤维粘连蛋白促进非小细胞肺癌发生发展中的机制(编号:81372507);; 建设高水平大学附属医院专项资金(编号:2016031638);; 深圳市科技计划项目(编号:JCYJ20160425105945739)共同资助
2018-03-25
2018-03-25