| 560 | 16 | 115 |
| 下载次数 | 被引频次 | 阅读次数 |
羽叶三七是五加科人参属的名贵药材,三萜皂苷为羽叶三七最主要的活性成分。为了探索羽叶三七根茎中皂苷物质生物合成的分子基础,采用Illumina Hi Seq 2000高通量测序获得羽叶三七根茎的转录组数据;使用Trinity和TGICL软件实现Uni Gene的de novo拼接;基于BLAST完成Uni Gene的蛋白功能注释、KOG功能注释、GO分类和KEGG代谢通路分析。最终通过de novo拼接注释得到Uni Gene 62 240个。研究发现,羽叶三七根茎部表达的26个Uni Gene与三萜碳环骨架合成相关;三萜合成通路中的关键酶FPS、SS、SE等,分别有11 114个Uni Gene。该研究发现的三萜皂苷合成相关候选基因对于阐明羽叶三七三萜皂苷合成方式研究提供了理论基础。
Abstract：Panax pseudoginseng is a valuable Chinese herbal medicine, belonging to Araliaceae family, Panax genus. Triterpene saponins are the most active components in Panax pseudoginseng. In order to explore the molecule basis of triterpenoid saponins biosynthesis in Panax pseudoginseng, the transcriptome data of rhizome of Panax pseudoginseng was obtained by Illumina Hi Seq 2000 high-throughput sequencing, and de novo splicing of Uni Gene was realized by Trinity and TGICL. The protein function annotation, KOG functional annotation, GO classification and metabolism of KEGG metabolic pathway analysis of Uni Gene were completed based on BLAST. 62 240 Uni Genes were finally obtained by de novo splicing and annotation. After annotation, we discovered 26 UniGenes expressed in rhizome of Panax pseudoginseng were involved in the biosynthesis of ginsenoside backbone.Additionally, there were 11 114 Uni Genes of the key enzymes FPS, SS, SE in triterpene saponins synthesis pathway.In conclusion, the related candidate genes of triterpenoid saponin synthesis found in this study would provide a theoretical basis for elucidating the synthesis of triterpenoid saponin in Panax pseudoginseng.
Ansorge W.J.,2009,Next-generation DNA sequencing techniques,N Biotechnol,25(4):195-203
Altschul S.F.,Gish W.,Miller W.,Myers E.W.,and Lipman D.J.,1990,Basic local alignment search tool,Journal of molecular biology,215(3):403-410
Chen S.L.,Lo H.M.,and Li Y.,2011,454 EST analysis detects genes putatively involved in ginsenoside biosynthesis in Panax ginseng,Plant Cell Rep.,30(9):1593-1601
Chen S.L.,Zhu X.X.,and Li C.F.,2012,Genomics and synthetic biology of traditional Chinese medicine,Yaoxue Xuebao(Acta Pharmaceutica Sinica),47(8):1070(陈士林,朱孝轩,李春芳,2012,中药基因组学与合成生物学,药学学报,47(8):1070)
Haas B.J.,Papanicolaou A.,Yassour M.,Grabherr M.,Blood P.D.,Bowden J.,Couger M.B.,Eccles D.,Li B.,and Lieber M.,2013,De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis,Nature Protocols,8(8):1494-1512
Jennings S.M.,Tsay Y.H.,Fisch T.M.,and Robinson G.W.,1991,Molecular cloning and characterization of the yeast gene for squalene synthetase,Proc.Natl.Acad.Sci.USA,88(14):6038-6042
Kim T.D.,Han J.Y.,Huh G.H.,and Choi Y.E.,2011,Expression and functional characterization of three squalene synthase genes associated with saponin biosynthesis in Panax gin seng,Plant Cell Physiol.,52(1):125-137
Li H.,Handsaker B.,Wysoker A.,Fennell T.,Ruan J.,Homer N.,Marth G.,Abecasis G.,and Durbin R.,2009,The sequence alignment/Map format and SAMtools,Bioinformatics,25(16):2078-2079
Li Y.,Luo H.M.,and Sun C.,2010,EST analysis reveals putative genes involved in glycyrrhizin biosynthesis,BMC Genomics,11:268
Luo H.M.,Sun C.,and Song J.Y.,2011,Analysis of the transcriptome of Panax notoginseng root uncovers putative triterpene saponin-biosynthetic genes and genetic markers,BMC Genomics,12(Suppl 5):S5
Lee M.H.,Jeong J.H.,Seo J.W.,Shin C.G.,Kim Y.S.,In J.G.,Yang D.C.,Yi J.S.,and Choi Y.E.,2004,Enhanced triterpene and phytos terol biosynthesis in Panax ginseng overexpressing squalene synthase gene,Plant Cell Physiol.,45(8):976-984
Li Y.,Zhao Y.Y.,and Yu H.P.,2010,The Chinese pharmacopoeia,Medical Technology Publisher,China,Beijing,pp.254(李燕,赵燕宜,于海平,2010,中国药典,医药科技版社,中国,北京,pp.254)
Li Q.Y.,Zhao H.,and Yue S.J.,1993,Analgesic and sedative effects of Dayezhuzishen,Huaxi Yaoxue Zazhi(West China Journal of Pharmaceutical Sciences),8(2):90-92(李巧云,赵恒,岳松健,1993,大叶珠子参总皂苷的镇痛镇静作用研究,华西药学杂志,8(2):90-92)
Lian J.,Zhang X.C.,and Gu J.T.,2015,Advance in transcriptomics and its application in olericulture research,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),31(8):118-122(廉洁,张喜春,谷建田,2015,转录组学及其在蔬菜学上应用研究进展,中国农学通报,31(8):118-122)
Lu H.Y.,Liu J.M.,Yang W.L.,and Gao S.L.,2007,Isolation of squalene synthase genes of Glycyrrhiza uralensis and construction of plant expression vector,14(4):255-258(卢虹玉,刘敬梅,阳文龙,高山林,2007,甘草鲨烯合成酶基因的分离及植物表达载体的构建,药物生物技术,14(4):255-258)
Martin M.,2011,Cutadapt removes adapter sequences from high-throughput sequencing reads,EMBnet,17(1):10-12
Ma S.J.,An C.,Xue W.J.,and Shangguan Y.Q.,2016,Deep sequencing-based transcriptome analysis of aureobasidium pullulans,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),4(35):931-941(马赛箭,安超,薛文娇,上官亦卿,2016,基于高通量测序的出芽短梗霉转录组学研究,基因组学与应用生物学,4(35):931-941)
Qian J.Y.,Ma C.T.,Chao N.X.,Chen Q.C.,Lan X.W.,Sun J.,and Wu S.Y.,2016,Cloning and positive selection analysis of squalene synthase gene ORF in green momordica charantia,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),8(36):1-11(钱洁颖,马成通,晁耐霞,陈奇聪,蓝秀万,孙健,吴耀生,2016,青皮苦瓜鲨烯合酶基因ORF序列的克隆和正选择分析,基因组学与应用生物学,8(36):1-11)
Sun C.,Li Y.,and Wu Q.,2010,De novo sequencing and analysis of the American ginseng root transcriptome using a GSFLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis,BMC Genomics,11:262
Wang D.Q.,1988,Saponins of the rhizome of Panax Japonicus var.bipinnatifidus and its significan t of chemotaxonmy,Yunnan Zhiwuyanjiu(Acta Botallica Yunnanica),10(1):120-204(王答祺,1988,羽叶三七根茎的三萜皂苷成分及其化学分类学意义,云南植物研究,10(1):120-204)
Yang X.,Jiao R.,Yang L.,Wu L.P.,Li Y.R.,and Wang J.,2011,New-generation high-throughput t ech nologies based'omics'research strategy in human disease,Yichuan(HEREDITAS),33(8):829-846(杨旭,焦睿,杨琳,吴莉萍,李英睿,王俊,2011,基于新一代高通量技术的人类疾病组学研究策略,遗传,33(8):829-846)
Zhao Y.,Zhao R.,Shan X.X.,Yang J.W.,Xu S.Z.,and Wen G.Z.,2012,Analysis of botanical characters and growth dynamic in Panax japonicus,Yunnanzhongyixuyuan Xuebao,Yunnan Zhongyixueyuan Xuebao(Journal of Yunnan University of Traditional Chinese Medicine),2(35):24-27(赵毅,赵仁,山学祥,杨杰武,徐绍忠,文国松,2012,羽叶三七植物性状及生长动态分析,云南中医学院学报,2(35):24-27)
Zhao R.,Zhao Y.,and Li D.M.,2008,The research progress of Panaxa japonicus C.A.Mey.varmajor(Burk.)C.Y.Wuet K.M.Feng,Zhongguo Xiandai Zhongyao(Modem Chinees Medie),10(10):3-6(赵仁,赵毅,李东明,2008,珠子参研究进展,中国现代中药,10(10):3-6)
Zhang S.P.,Jin J.,Hu B.X.,Wu Y.Y.,Yan Q.,Zeng W.Y.,Zheng Y.L.,Zhang X.F.,and Chen P.,2015,Transcriptome profiling and analysis of Panax japonicus var.major,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materia Medica),11(40):2084-2089(张绍鹏,金健,胡炳雄,吴亚运,闫祺,曾万勇,郑用琏,张西峰,陈平,2015,珍稀药用植物珠子参的转录组测序及分析,中国中药杂志,11(40):2084-2089)
Zhang Z.Q.,Shan X.X.,Li D.M.,Wang J.,Zhao Y.,and Zhao R.,2011,The research of standardized cultivation biology for Panax japonicus C.A.M eyer.var major(Burk.)C.Y.Wu et K.M.F rng and Panax japonicus Meyer.var.bipinnati fidus(Seem.)C.Y.Wu et K.M.Feng,Yunnan Zhongyi Zhongyao Zazhi(Yunnan Journal of Traditional Chinese Medicine and Materia Medica),9(32):34-36(张志清,山学祥,李东明,王俊,赵毅,赵仁,2011,珠子参、羽叶三七规范化栽培生物学研究,云南中医中药杂志,9(32):34-36)
基本信息:
DOI：10.13417/j.gab.036.002531
中图分类号:Q943.2;S567.5
引用信息:
[1]黄勋,金健,余洁,等.羽叶三七的转录组测序与三萜皂苷生物合成的关键酶基因的识别[J].基因组学与应用生物学,2017,36(06):2531-2538.DOI:10.13417/j.gab.036.002531.
基金信息:
国家自然科学基金(No.81274023)资助
2016-10-13
2016-10-13
2016-10-13