基因组学与应用生物学

为了评估肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白I(cTnI)、肌红蛋白(Myo)和N端脑钠肽前体(NT-proBNP)这4种体外诊断试剂在诊断急性心肌梗死中的价值,本研究检测了200例急性心肌梗死患者和100例健康受试者血清中各项指标的水平。研究结果显示,急性心肌梗死患者的血清CK-MB、cTnI、Myo和NT-proBNP水平依次为36.74 ng/mL、14.29 ng/mL、381.96μg/mL和1 375.54 pg/mL,而健康受试者的血清CK-MB、cTnI、Myo和NT-proBNP水平依次为3.21 ng/mL、0.33 ng/mL、65.2μg/mL和72.78 pg/mL。心肌梗死患者的上述4种指标均显著高于健康受试者(p<0.05)。4项指标中,cTnI的敏感度、特异性和曲线下面积均为最高,依次为0.764、0.719和0.747。此外,4项指标联合诊断的敏感度、特异性和曲线下面积依次为0.836、0.812和0.828,均高于单独指标诊断。本研究说明CK-MB、cTnI、Myo和NT-proBNP水平在急性心肌梗死患者的血清中明显升高,并且4项指标联合检测有利于急性心肌梗死的早期诊断。

To evaluate the value of four in vitro diagnostic reagents, creatine kinase-MB(CK-MB), cardiac troponin I(cTnI), myoglobin(Myo) and N-terminal pro-B type natriuretic peptide(NT-proBNP), in the diagnosis of acute myocardial infarction. This study examined the levels of various indicators in the serum of 200 patients with acute myocardial infarction and 100 healthy subjects. Studies have shown that serum CK-MB, cTnI, Myo, and NT-proBNP levels in patients with acute myocardial infarction are 36.74 ng/mL, 14.29 ng/mL, 381.96 μg/mL, and1 375.54 pg/mL, respectively, while the levels of serum CK-MB, cTnI, Myo, and NT-proBNP in healthy subjects were 3.21 ng/mL, 0.33 ng/mL, 65.2 μg/mL, and 72.78 pg/mL, respectively. The above four indexes in patients with myocardial infarction were significantly higher than those of healthy subjects(p<0.05). Among the four indicators,the sensitivity, specificity and area under the curve of cTnI were the highest, followed by 0.764, 0.719 and 0.747.In addition, the sensitivity, specificity, and area under the curve of the combined diagnosis of the four indicators were 0.836, 0.812, and 0.828, respectively, which were higher than the individual indicators. These results indicate that the levels of CK-MB, cTnI, Myo and NT-proBNP are significantly elevated in the serum of patients with acute myocardial infarction, and the combined detection of four indicators is conducive to the early diagnosis of acute myocardial infarction.