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野油菜黄单胞菌野油菜致病变种(简称Xcc)是十字花科作物黑腐病的病原菌,同时也是发酵工业生产黄原胶的菌种。在以前的工作中,证实了Xcc8004菌株基因组中一个包含XC3813-3815三个基因的新位点与致病性和胞外多糖(EPS,又称黄原胶)合成有关。本工作通过PCR合成包含相应ORF及其上游不同长度的DNA片段,并克隆于不带启动子的质粒载体pLAFR6后,将所获的重组质粒互补相应的突变体。通过这一功能互补的方法,确定了这三个基因在其ORF上游约50bp内分别拥有自身的启动子。
Abstract：Xanthomonas campestris pv.campestris(Xcc) is the causal agency of the black rot disease of cruciferous plants,and the microorganism used for xanthan gum production in ferment industry.In previous work a novel locus,XC3813-3815,had been identified to be required for full virulence and extracellular polysaccharide(EPS,also called xanthan gum) production.Here the promoter region of XC3813-3815 was investigated.DNA fragments spanning downstream and different length of upstream region of open reading frame(ORF) were amplified by PCR and cloned into the promoterless plasmid pLAFR6,respectively.The resulting recombinant plasmids were introduced into the corresponding mutants for the complementation of these three genes mutants.By this method of functional complementation,the promoter for each of the three genes was limited to 50 bp upstream region of the corresponding ORF.
[1]SWINGS J G,CIVEROLO E L.Xanthomonas[M].London:Chapman&Hall,1993.
[2]KENNEDYJ F,BRADSHAWI J.Production,properties and application of xanthan[J].Prog Ind Microbiol,1984,19:319-371.
[3]LU G T,MA Z F,HU J R,et al.A novel locus involved in extracellular polysaccharide production andvirulence ofXanthomonas campestrispathovarcampestris[J].Microbiol,2007,153(3):737-746.
[4]谭旖宁,马增凤,陆光涛,等.野油菜黄单胞菌6-磷酸葡萄糖脱氢酶基因的初步分析[J].广西农业生物科学,2007,26(3):190-195.
[5]DANIELS MJ,BARBER C E,TURNER P C,et al.Cloning of genes involvedin pathogenicity ofXanthomonascampestrispv.campestrisusing the broad host range cosmid pLAFR1[J].EMBOJ,1984,3:3323-3328.
[6]HUYNH T V,DAHLBECK D,STASKAWICZ B J.Bacterial blight of soybean:regulation of a pathogen genedetermining host cultivar specificity[J].Science,1989,245:1374-1377.
[7]SAMBROOK J,RUSSE D W.Molecular cloning:a laboratory manual[M].3rd ed.New York:Cold SpringHarbor Laboratory Press,2001.
[8]TURNER P,BARBER C,DANNIELS M.Behaviour of the transposans Tn5 and Tn7 inXanthomonascampestrispv.campestris[J].Mol Gen Genet,1984,195:101-107.
[9]王亚馥,戴灼华.遗传学[M].北京:高等教育出版社,1999.
基本信息:
中图分类号:S435.654
引用信息:
[1]陆光涛,彭方印,李彩月,等.野油菜黄单胞菌XC3813-3815基因启动子的定位分析[J].广西农业生物科学,2008(02):104-109.
基金信息:
国家自然科学基金重点项目(30130010);; 国家自然科学基金面上项目(30470944)
2008-06-15
2008-06-15