基因组学与应用生物学

本研究探讨了乙二胺四乙酸钠(EDTA-N a)对猪卵母细胞的体外成熟和孤雌激活后早期发育的影响。利用屠宰场猪卵巢卵母细胞,在不添加或添加不同浓度EDTA-N a的成熟培养液中体外成熟44~48 h,挑选核成熟卵母细胞进行孤雌激活,然后移到不添加或添加不同浓度EDTA-N a的胚胎培养液中进行体外培养168 h。结果说明:(1)成熟液中添加适当浓度(25~100μm o l/L)EDTA-N a对体外成熟猪卵母细胞孤雌激活后的早期发育具有促进作用,在EDTA-N a浓度为100μm o l/L时,效果最佳;(2)胚胎培养液中添加适当浓度(25~100μm o l/L)EDTA-N a,对体外成熟猪卵母细胞孤雌激活后的早期发育具有促进作用,在EDTA-N a浓度为100μm o l/L时,效果最佳;(3)成熟液和胚胎培养液中同时添加100μm o l/L EDTA-N a对体外成熟猪卵母细胞孤雌激活后的早期发育具有促进作用;(4)成熟液或/和胚胎培养液中添加不同浓度EDTA-N a,对体外成熟培养后的猪核成熟卵母细胞及孤雌激活后的4-细胞孤雌胚SDS-PAGE电泳的蛋白质表达图谱都无显著影响。

This study was to investigate the effect of EDTA-Na on in vitro maturation of porcine oocytes and early development after parthenogenetically activation.Porcine oocytes from abattoir ovaries were matured in maturation medium whether or not containing different concentrations of EDTA-Na for 44~ 48 hours.Oocytes with the first polar body were parthenogenetically activated and then cultured in NCSU-23 medium whether or not containing different concentrations ofEDTA-Na.The results showed:(1) Supplementation with 25~100 μmol/L EDTA-Na in maturation medium was beneficial to early development of porcine parthenogenetic embryos derived from oocytes matured and artificially activated in vitro,and when EDTA-Na concentration was 100 μmol/L its effect was the best;(2) Supplementation with 25~100 μmol/L EDTA-Na in embryo culture medium was beneficial to early development of porcine parthenogenetic embryos derived from oocytes matured and artificially activated in vitro,and when EDTA-Na concentration was 100 μmol/L its effect was the best;(3) Supplementation with 100 μmol/L EDTA-Na in maturation and embryo culture media together was benificial to early development of porcine parthenogenetic embryos derived from oocytes matured and artificially activated in vitro;(4) Supplementation with different concentrations of EDTA-Na in maturation medium or/and embryo culture medium had no significant effect on protein expression profiles of SDS-PAGE electrophoresis of porcine oocytes matured in vitro and 4-cell parthenogenetic embryos after artificial activated.