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为了解叶绿素合成关键酶基因在大豆不同生育期的表达情况,本研究以拟南芥叶绿素合成关键酶基因序列设计引物,大豆科丰14和它的持绿突变体BN108为试材,利用半定量RT-PCR分析了9种叶绿素合成关键酶基因在开花期和成熟前期表达量的变化。结果表明,HEMA、GSA、HEME、POR、CHLG基因在成熟前期表达量显著下降(p<0.05),尤其是HEMA和GSA基因的表达量下降幅度较大,说明上述基因在叶绿素生物合成过程中起到主要调控作用;而突变体BN108有别于科丰14,GSA、HEME、CHLM和CAO基因表达量在成熟前期不降反升,推测持绿突变体在成熟前期叶绿素能够维持较大的合成量,是植物持绿现象的重要原因之一。
Abstract：The objective of this study was to dissect the expression variation of key enzyme gene involved in chlorophyll biosynthesis at different development stages of soybean. Primers were designed according to the key enzyme gene sequence of chlorophyll biosynthesis in Arabidopsis, semi-quantitative RT-PCR was employed to analyze expression variations of key enzyme gene in chlorophyll biosynthesis at flowering and early maturity stages for KF14 and its stay-green mutant BN108 in soybean. The results showed that gene expression levels of HEMA, GSA, HEME, POR and CHLG in KF14, especially for HEMA and GSA had significant decline(p<0.05)during early maturity stage than during flowering stage. It meant above chlorophyll biosynthesis genes that might play a major role in key enzyme genes. However, gene expression levels of GSA, HEME, CHLM and CAO in stay-green mutant BN108, unlike KF14, had significant raise(p<0.05) during early maturity stage. It would infer that synthetic content of chlorophyll in stay-green mutant was not reduced during early maturity stage, which might be one of the important reasons for stay-green phenomenon in plant.
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基本信息:
DOI：10.13417/j.gab.034.000593
中图分类号:Q943.2
引用信息:
[1]姚嘉龙,郭蓓,谢皓.叶绿素合成关键酶基因表达的半定量RT-PCR分析[J].基因组学与应用生物学,2015,34(03):593-598.DOI:10.13417/j.gab.034.000593.
基金信息:
国家自然科学基金(31371648)资助
2015-03-25
2015-03-25