| 452 | 2 | 74 |
| 下载次数 | 被引频次 | 阅读次数 |
BAG(Bcl-2 associated athanogene)在真核生物中是一种保守的辅助伴侣蛋白。本研究在甘蔗割手密(Saccharum spon-taneum)基因组中鉴定获得24个BAG基因，分布在19条染色体上，根据其系统发育关系可分为3个组。研究发现，全基因组复制或片段复制是该家族的主要扩张方式。顺式作用元件预测分析表明，SsBAGs主要参与植物激素调节、非生物胁迫响应、光响应和植物生长发育等。转录表达谱分析表明，多数SsBAGs在茎中的表达量显著高于叶片中的，而在叶片的库源过渡区域的表达高于叶片其他区域的。此外，不同SsBAGs在干旱胁迫下的表达存在差异。亚细胞定位表明，ScBAG1a和ScBAG2a均定位于细胞质和细胞核，与生物信息学分析结果一致。值得指出的是，BAG蛋白的结构域和BAG基因的表达模式在甘蔗和水稻(Oryza sativa)中均相当保守。甘蔗BAG基因家族扩张所造成的家族成员功能冗余在一定程度上可能与甘蔗作为多年生作物的抗逆性、抗病性和再生性有关。本研究初步揭示了SsBAG基因在甘蔗割手密中的生长发育和逆境胁迫响应中发挥的功能，为进一步研究其作用机制奠定了基础。
Abstract：BAG(Bcl-2 associated athanogene) is a conserved co-chaperone protein in eukaryotes. In this study, 24 BAG genes were identified in sugarcane Saccharum spontaneum genome, which were distributed on 19 chromosomes and could be divided into three groups according to their phylogenetic relationships. It was found that whole genome duplication or segmental duplication was the main expansion mode of this family. The prediction analysis of cis regulatory element showed that SsBAGs were mainly involved in plant hormone regulation, abiotic stress response, light response, growth and development of plant and so on. Transcriptional expression profile analysis showed the most of SsBAGs were expressed in stems, much significantly higher than in leaves, and the expression of SsBAGs in sink-source transition region of leaves was higher than in the other regions of leaves. In addition, the different members of SsBAGs family had distinct patterns of expression under drought stress. Subcellular localization analysis showed that both ScBAG1a and ScBAG2a were localized in cytoplasm and nucleus, which was consistent with the results of bioinformatics prediction. It is noteworthy that the domains of BAG protein and the expression pattern of BAG genes are highly conserved in both sugarcane and rice(Oryza sativa). The functional redundancy of family members caused by the expansion of BAG gene family may be related to the stress resistance, disease resistance and regeneration of sugarcane as a perennial crop. These results proposed the roles of SsBAGs in the plant growth and develop-ment and stress response of sugarcane Saccharum spontaneum, which laid a foundation for further revealing their molecular function in sugarcane.
张扬，杜琳，唐贤丰，等，2019.杨树BAG基因的鉴定及表达模式分析.林业科学，55(1):138-145.[ZHANG Y,DU L,TANG X F,et al.,2019.Identification and expression pattern analyses of Populus BAG genes.Scientia Silvae Sinicae,55(1):138-145.]
ALI A,KHAN M,SHARIF R,et al.,2019.Sugarcane omics:an update on the current status of research and crop improvement.Plants,8(9):344.
ARIF M,LI Z,LUO Q,et al.,2021.The BAG2 and BAG6 genes are involved in multiple abiotic stress tolerances in Arabidopsis thaliana.Int.J.Mol.Sci.,22(11):5856.
BAILEY T L,JOHNSON J,GRANT C E,et al.,2015.The MEME suite.Nucleic.Acids Res.,43(W1):W39-W49.
BRIVE L,TAKAYAMA S,BRIKNAROVá K,et al.,2001.The carboxyl-terminal lobe of Hsc70 ATPase domain is sufficient for binding to BAG1.Biochem.Biophys.Res.Commun.,289(5):1099-1105.
CHAO J,LI Z,SUN Y,et al.,2021.MG2C:a user-friendly online tool for drawing genetic maps.Mol.Hortic.,1(1):16.
CHEN C J,CHEN H,ZHANG Y,et al.,2020.TBtools:an integrative toolkit developed for interactive analyses of big bio-logical data.Mol.Plant,13(8):1194-1202.
CHOU K C,SHEN H B,2010.Cell-PLoc 2.0:an improved package of web-servers for predicting subcellular localization of proteins in various organisms.Nat.Sci.,2(10):1090-1103.
DASH A,GHAG S B,2022.Genome-wide in silico characterization and stress induced expression analysis of BcL-2 associa-ted athanogene (BAG) family in Musa spp.Sci.Rep.,12(1):625.
D'HONT A,ISON D,ALIX K,et al.,1998.Determination of basic chromosome numbers in the genus Saccharum by physical mapping of ribosomal RNA genes.Genome,41(2):221-225.
DING H D,QIAN L,JIANG H L,et al.,2022.Overexpression of a Bcl-2-associated athanogene SlBAG9 negatively regulates high-temperature response in tomato.Int.J.Biol.Macromol.,194:695-705.
DOUKHANINA E V,CHEN S R,VAN DER ZALM E,et al.,2006.Identification and functional characterization of the BAG protein family in Arabidopsis thaliana.J.Biol.Chem.,281(27):18793-18801.
DUARTE J M,CUI L Y,WALL P K,et al.,2006.Expression pattern shifts following duplication indicative of subfunctiona-lization and neofunctionalization in regulatory genes of Arabidopsis.Mol.Biol.Evol.,23(2):469-478.
FANG S S,LI L H,CUI B Y,et al.,2013.Structural insight into plant programmed cell death mediated by BAG proteins in Arabidopsis thaliana.Acta Crystallogr.Sect.D Biol.Crystallogr.,69(Pt 6):934-945.
FLAGEL L E,WENDEL J F,2009.Gene duplication and evolutionary novelty in plants.N.Phytol.,183(3):557-564.
GASTEIGER E,HOOGLAND C,GATTIKER A,et al.,2005.Protein identification and analysis tools on the ExPASy ser-ver//Walker J M.The Proteomics Protocols Handbook.Clifton,New Jersey:Humana Press:571-607.
HE M M,WANG Y,JAHAN M S,et al.,2021.Characterization of SlBAG genes from Solanum lycopersicum and its function in response to dark-induced leaf senescence.Plants,10(5):947.
HOANG T M L,MOGHADDAM L,WILLIAMS B,et al.,2015.Development of salinity tolerance in rice by constitutive-overexpression of genes involved in the regulation of programmed cell death.Front.Plant Sci.,6:175.
HORTON P,PARK K J,OBAYASHI T,et al.,2007.WoLF PSORT:protein localization predictor.Nucleic Acids Res.,35(Suppl_2):W585-W587.
HU L Z,CHEN J T,GUO J J,et al.,2013.Functional divergence and evolutionary dynamics of BAG gene family in Maize (Zea mays).Int.J.Agric.Biol.,15(2):200-206.
HU W C,HUA X T,ZHANG Q,et al.,2018.New insights into the evolution and functional divergence of the SWEET family in Saccharum based on comparative genomics.BMC Plant Biol.,18(1):270.
IRFAN M,KUMAR P,AHMAD I,et al.,2021.Unraveling the role of tomato Bcl-2-associated athanogene (BAG) proteins during abiotic stress response and fruit ripening.Sci.Rep.,11:21734.
JAIN S,WIEMANN P,THILL E,et al.,2018.A bcl-2 associa-ted athanogene (bagA) modulates sexual development and secondary metabolism in the filamentous fungus Aspergillus nidulans.Front.Microbiol.,9:1316.
KABBAGE M,KESSENS R,BARTHOLOMAY L C,et al.,2017.The life and death of a plant cell.Annu.Rev.Plant Biol.,68:375-404.
KANG C H,JUNG W Y,KANG Y H,et al.,2006.AtBAG6,a novel calmodulin-binding protein,induces programmed cell death in yeast and plants.Cell Death Differ.,13(1):84-95.
KOKKIRALA V R,PENG Y G,ABBAGANI S,et al.,2010.Subcellular localization of proteins of Oryza sativa L.in the model tobacco and tomato plants.Plant Signal.Behav.,5(11):1336-1341.
KRZYWINSKI M,SCHEIN J,BIROL I,et al.,2009.Circos:an information aesthetic for comparative genomics.Genome Res.,19(9):1639-1645.
KUMAR S,STECHER G,LI M,et al.,2018.MEGA X:mole-cular evolutionary genetics analysis across computing platforms.Mol.Biol.Evol.,35(6):1547-1549.
LAM E,SHINE J,SILVA J D,et al.,2009.Improving sugarcane for biofuel:engineering for an even better feedstock.GCB Bioenergy,1(3):251-255.
LEE D W,KIM S J,OH Y J,et al.,2016.Arabidopsis BAG1 functions as a cofactor in Hsc70-mediated proteasomal degradation of unimported plastid proteins.Mol.Plant,9(10):1428-1431.
LESCOT M,DéHAIS P,THIJS G,et al.,2002.PlantCARE,a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences.Nucleic Acids Res.,30(1):325-327.
LI L H,XING Y F,CHANG D,et al.,2016a.CaM/BAG5/Hsc70 signaling complex dynamically regulates leaf senescence.Sci.Rep.,6:31889.
LI P T,CHAI Z,LIN P P,et al.,2020a.Genome-wide identification and expression analysis of AP2/ERF transcription factors in sugarcane (Saccharum spontaneum L.).BMC Ge-nom.,21(1):685.
LI P T,LIN P P,ZHAO Z L,et al.,2022.Gene co-expression analysis reveals transcriptome divergence between wild and cultivated sugarcane under drought stress.Int.J.Mol.Sci.,23(1):569.
LI Y R,KABBAGE M,LIU W D,et al.,2016b.Aspartyl protease-mediated cleavage of BAG6 is necessary for autophagy and fungal resistance in plants.Plant Cell,28(1):233-247.
LI Y R,WILLIAMS B,DICKMAN M,2017.Arabidopsis B-cell lymphoma2 (Bcl-2)-associated athanogene 7 (BAG7)-mediated heat tolerance requires translocation,sumoylation and binding to WRKY29.N.Phytol.,214(2):695-705.
LI Z,HUA X T,ZHONG W M,et al.,2020b.Genome-wide identification and expression profile analysis of WRKY family genes in the autopolyploid Saccharum spontaneum.Plant Cell Physiol.,61(3):616-630.
LING H,WU Q B,GUO J L,et al.,2014.Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative RT-PCR.PLoS ONE,9(5):e97469.
NAWKAR G M,MAIBAM P,PARK J H,et al.,2017.In silico study on Arabidopsis BAG gene expression in response to environmental stresses.Protoplasma,254(1):409-421.
PANJE R R,BABU C N,1960.Studies in Saccharum sponta-neum distribution and geographical association of chromosome numbers.CYTOLOGIA,25(2):152-172.
ROACH B T,1972.Chromosome numbers in Saccharum edule.CYTOLOGIA,37(1):155-161.
SONDERMANN H,HO A K,LISTENBERGER L L,et al.,2002.Prediction of novel Bag-1 homologs based on structure/function analysis identifies Snl1p as an Hsp70 co-chaperone in Saccharomyces cerevisiae.J.Biol.Chem.,277(36):33220-33227.
TAKAYAMA S,SATO T,KRAJEWSKI S,et al.,1995.Cloning and functional analysis of BAG-1:a novel Bcl-2-binding protein with anti-cell death activity.Cell,80(2):279-284.
THANTHRIGE N,JAIN S,BHOWMIK S D,et al.,2020.Centrality of BAGs in plant PCD,stress responses,and host defense.Trends Plant Sci.,25(11):1131-1140.
TOWNSEND P A,CUTRESS R I,SHARP A,et al.,2003.BAG-1:a multifunctional regulator of cell growth and sur-vival.Biochim.Biophys.Acta (BBA) Rev.Cancer,1603(2):83-98.
VAN DE PEER Y,MIZRACHI E,MARCHAL K,2017.The evolutionary significance of polyploidy.Nat.Rev.Genet.,18(7):411-424.
WANG J,NAN N,LI N,et al.,2020a.A DNA methylation reader-chaperone regulator-transcription factor complex activates OsHKT1;5 expression during salinity stress.Plant Cell,32(11):3535-3558.
WANG J Y,YECKEL G,KANDOTH P K,et al.,2020b.Targeted suppression of soybean BAG6-induced cell death in yeast by soybean cyst nematode effectors.Mol.Plant Pathol.,21(9):1227-1239.
WANG P P,MOORE B M,PANCHY N L,et al.,2018.Factors influencing gene family size variation among related species in a plant family,Solanaceae.Genome Biol.Evol.,10(10):2596-2613.
WANG Y P,TANG H B,DEBARRY J D,et al.,2012.MCScanX:a toolkit for detection and evolutionary analysis of gene synteny and collinearity.Nucleic Acids Res.,40(7):e49.
WAPINSKI I,PFEFFER A,FRIEDMAN N,et al.,2007.Natural history and evolutionary principles of gene duplication in fungi.Nature,449(7158):54-61.
WILLIAMS B,KABBAGE M,BRITT R,et al.,2010.AtBAG7,an Arabidopsis Bcl-2-associated athanogene,resides in the endoplasmic reticulum and is involved in the unfolded protein response.Proc.Natl.Acad.Sci.USA,107(13):6088-6093.
WU S D,HAN B C,JIAO Y N,2020.Genetic contribution of paleopolyploidy to adaptive evolution in angiosperms.Mol.Plant,13(1):59-71.
YUAN Y,YANG X P,FENG M F,et al.,2021.Genome-wide analysis of R2R3-MYB transcription factors family in the autopolyploid Saccharum spontaneum:an exploration of dominance expression and stress response.BMC Genom.,22(1):622.
ZHANG J S,ZHANG X T,TANG H B,et al.,2018.Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L.Nat.Genet.,50(11):1565-1573.
ZHOU H L,LI J Y,LIU X Y,et al.,2021.The divergent roles of the rice bcl-2 associated athanogene (BAG) genes in plant development and environmental responses.Plants,10(10):2169.
基本信息:
DOI：10.13417/j.gab.042.001364
中图分类号:S566.1
引用信息:
[1]莫心怡,周海连,何丹丹,等.甘蔗割手密BAG基因家族的鉴定与表达分析[J].基因组学与应用生物学,2023,42(12):1364-1378.DOI:10.13417/j.gab.042.001364.
基金信息:
广西大学甘蔗专项科研项目(2022GZA001)资助
2023-05-05
2023-05-05
2023-05-05