nav emailalert searchbtn searchbox tablepage yinyongbenwen piczone journalimg journalInfo journalinfonormal searchdiv searchzone qikanlogo popupnotification paper paperNew
2017, 07, v.36 2862-2866
磷酸化蛋白质组学在哺乳动物精子研究中的应用
基金项目(Foundation): 国家自然科学基金(31160421);; 广西高等学校高水平创新团队及卓越学者计划项目(201338)共同资助
邮箱(Email):
DOI: 10.13417/j.gab.036.002862
摘要:

蛋白质磷酸化是蛋白质翻译后最普遍、最重要的修饰之一,是生物体内一种普通的调节方式,参与调控细胞增殖、信号转导、新陈代谢、肿瘤发生等分子机能,并在精子信号转导和酶合成表达的过程中起重要作用。对精子磷酸化蛋白的研究有助于深入了解精子发生、运输、获能,以及精卵识别的调控机理。因此,在磷酸化蛋白组学的层面上研究精子的各项机能可以为雄性不育更深层的研究提供一条新的道路。

Abstract:

Phosphorylation is one of the most common and important post-translational modifications of proteins,which is an ordinary way of organism regulation and participates in controlling molecular mechanism such as cell proliferation, signal transduction, metabolism, and tumorigenesis. Furthermore, it plays an important role in the process of sperm signal transduction and the expression of enzymatic synthesis. Researches about phosphorylated proteins of sperm contribute to the further understanding of the molecular mechanism of spermatogenesis,transportation, capacitation, and sperm-egg recognition. Therefore, the study of the functions of sperm on phosphoproteomics level could provide a new way for further research on male sterility.

参考文献

Brandt H.,and Hoskins D.D.,1980,A camp-dependent phosphorylated motility protein in bovine epididymal sperm,Journal of Biological Chemistry,255(3):982-987

Baker M.A.,Smith N.D.,Hetherington L.,Taubman K.,Graham M.E.,Robinson P.J.,and Aitken R.J.,2010,Label-free quantitation of phosphopeptide changes during rat sperm capacitation,Journal of Proteome Research,9(2):718-729

Diella F.,Gould C.M.,Chica C.,Via A.,and Gibson T.J.,2011,Phospho.ELM:a database of phosphorylation sites-update 2008,Nucleic Acids Research,36(Database issue):D240-D244

Fardilha M.,Esteves S.L.,Korrodi-Gregório L.,da Cruze Silva O.A.,and da Cruze Silva F.F.,2010,The physiological relevance of protein phosphatase 1 and its interacting proteins to health and disease,Current Medicinal Chemistry,17(33):3996-4017

Ficarro S.,Chertihin O.,Westbrook V.A.,White F.,Jayes F.,Kalab P.,Marto J.A.,Shabanowitz J.,Herr J.C.,Hunt D.F.,and Visconti P.E.,2003,Phosphoproteome analysis of capacitated human sperm.evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein pp.97 during capacitation,Journal of Biological Chemistry,278(13):11579-11589

Gerrits B.,and Bodenmiller B.,2010,Mapping of phosphorylation sites by LC-MS/MS,Methods in Molecular Biology,658:127-136

Gnad F.,Gunawardena J.,and Mann M.,2011,Phosida 2011:the posttranslational modification database,Nucleic Acids Research,39(Database issue):D253-D260

Hoskins D.D.,Casillas E.R.,and Stephens D.T.,1972,Cyclic amp-dependent protein kinases of bovine epididymal spermatozoa,Biochemical&Biophysical Research Communications,48(6):1131-1138

Hornbeck P.V.,Zhang B.,Murray B.,Ko rnhauser J.M.,Latham V.,and Skrzypek E.,2015,Phospho Site Plus,2014:mutations,PTMs and recalibrations,Nucleic Acids Res.,43(Database issue):D512-D520

Hunter T.,2000,Signaling-2000 and beyond,Cell,100(1):113-127

Ingles C.J.,and Dixon G.H.,1967,Phosphorylation of protamine during spermatogenesis in trout testis,Proceedings of the National Academy of Sciences,58(3):1011-1018

Johnson L.,1995,Efficiency of spermatogenesis,Microscopy Research&Technique,32(5):385-422

Jedrychowski M.P.,Huttlin E.L.,Haas W.,Sowa M.E.,Rad R.,and Gygi S.P.,2011,Evaluation of hcd-and cid-type fragmentation within their respective detection platforms for murine phosphoproteomics,Molecular&Cellular Proteomics,10(12):227-230

Krupa A.,Preethi G.N.,and Srinivasan N.,2004,Structural modes of stabilization of permissive phosphorylation sites in protein kinases:distinct strategies in ser/thr and tyr kinases,Journal of Molecular Biology,339(5):1025-1039

Kim J.H.,Lee J.,Oh B.,Kimm K.,and Koh I.,2004,Prediction of phosphorylation sites using svms,Bioinformatics,20(20):3179-3184

Kobor M.S.,and Greenblatt J.,2002,Regulation of transcription elongation by phosphorylation,Biochimica Et Biophysica Acta,1577(1577):261-275

Lardy H.A.,Garbers D.L.,Lust W.D.,and First N.L.,1971,Effects of phosphodiesterase inhibitors and cyclic nucleotides on sperm respiration and motility,Biochemistry,10(10):1825-1831

Leyton L.,and Saling P.,1989,95 kd sperm proteins bind zp3and serve as tyrosine kinase substrates in response to zona binding,Cell,57(7):1123-1130

Lau P.N.I.,and Cheung P.,2013,Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity,Nucleic Acids Research,41(3):e49

Mandal A.,Naabyhansen S.,Wolkowicz M.J.,Klotz K.,Shetty J.,Retief J.D.,Coonrod S.A.,Kinter M.,Sherman N.,Cesar F.,Flickinger C.J.,and Herr J.C.,1999,Fsp95,a testis-specific95-kilodalton fibrous sheath antigen that undergoes tyrosine phosphorylation in capacitated human spermatozoa,Biology of Reproduction,61(5):1184-1197

Moorhead G.B.,De W.V.,Templeton G.,and Kerk D.,2009,Evolution of protein phosphatases in plants and animals,Biochemical Journal,417(2):401-409

Nilsson C.L.,2012,Advances in quantitative phosphoproteomics,Analytical Chemistry,84(2):735-746

Naaby-Hansen S.,Flickinger C.J.,and Herr J.C.,1997,Two-dimensional gel electrophoretic analysis of vectorially labeled surface proteins of human spermatozoa,Biology of Reproduction,56(3):771-787

Ohkura H.,2004,Phosphorylation:polokinase joins an elite club,Current Biology,13(23):912-914

Platt M.,Salicioni A.,Hunt D.,and Visconti P.,2009,Use of differential isotopic labeling and mass spectrometry to analyze capacitation-associated changes in the phosphorylation status of mouse sperm proteins,Journal of Proteome Research,8(3):1431-1440

Puri P.,Myers K.,Kline D.,and Vijayaraghavan S.,2008,Proteomic analysis of bovine sperm ywha binding partners identify proteins involved in signaling and metabolism,Biology of Reproduction,79(6):1183-1191

Ruvolo P.P.,Deng X.,and May W.S.,2001,Phosphorylation of bcl2 and regulation of apoptosis,Leukemia,15(4):515-522

Szpunar J.,2005,Advances in analytical methodology for bioinorganic speciation analysis:metallomics,metalloproteomics and heteroatom-tagged proteomics and metabolomics,Analyst,130(4):442-465

Visconti P.E.,Bailey J.L.,Moore G.D.,Pan D.,Oldsclarke P.,and Kopf G.S.,1995,Capacitation of mouse spermatozoa.i.correlation between the capacitation state and protein tyrosine phosphorylation,Development,121(121):1129-1137

Wang F.,Song C.,Cheng K.,Jiang X.,Ye M.,and Zou H.,2011,Perspectives of comprehensive phosphoproteome analysis using shotgun strategy,Analytical Chemistry,83(21):8078-8085

Wang J.,Qi L.,Huang S.,Zhou T.,Guo Y.,Wang G.,Guo X.,Zhou Z.,and Sha J.,2015,Quantitative phosphoproteomics analysis reveals a key role of igf1r tyrosine kinase in human sperm capacitation,Molecular&Cellular Proteomics,14(4):1104-1112

Wasinger V.C.,Cordwell S.J.,Cerpa-Poljak A.,Yan J.X.,Gooley A.A.,Wilkins M.R.,Duncan M.W.,Harris R.,Williams K.L.,and Humphery-Smith I.,1995,Progress with gene-product mapping of the mollicutes:mycoplasma genitalium,Electrophoresis,16(1):1090-1094

Yang C.Y.,Chang C.H.,Yu Y.L.,Lin T.C.,Lee S.A.,Yen C.C.,Yang J.M.,Lai J.M.,Hong Y.R.,Tseng T.L.,Chao K.M.,and Huang C.Y.,2008,Phosphopoint:a comprehensive human kinase interactome and phospho-protein database,Bioinformatics,24(16):i14-i20

基本信息:

DOI:10.13417/j.gab.036.002862

中图分类号:S814.1

引用信息:

[1]张鹏飞,濮黎萍,王焕景,等.磷酸化蛋白质组学在哺乳动物精子研究中的应用[J].基因组学与应用生物学,2017,36(07):2862-2866.DOI:10.13417/j.gab.036.002862.

基金信息:

国家自然科学基金(31160421);; 广西高等学校高水平创新团队及卓越学者计划项目(201338)共同资助

发布时间:

2017-07-25

出版时间:

2017-07-25

检 索 高级检索