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本研究以濒危药用植物美花石斛种子萌发苗为外植体,考察不同基本培养基、植物生长激素、天然添加物、蔗糖浓度和活性炭对其壮苗构建的影响。研究结果表明:无菌萌发100 d左右的美花石斛种子苗,其最佳的壮苗培养基配方是1/2 MS+0.5 mg/L NAA+30%椰汁+30 g/L蔗糖+1 g/L活性炭+琼脂7 g/L,p H为5.8。
Abstract：This study investigated the influence of different factors(including minimal medium, plant growth hormone, natural additives, sugar concentration and activated carbon) on the seedling, using the seed germination seedling of endangered medicinal plant Dendrobium loddigesii Rolfe as explant. The result showed that the 1/2 MS with 0.5 mg/L NAA, 30%(v/v) coconut juice and 30 g/L sugar, 1 g/L activated carbon and 7 g/L agar with p H of5.8 was the best strengthening medium for the seed germination seedling of D. loddigesii about 100 days.
Editorial committee of Chinese journal of plant of Chinese acade my of sciences,ed.,1999,Flora of China(Vol.19),Scienc Press,Beijing,China,pp.104(中国科学院中国植物志编辑委员会,1999,中国植物志(第十九卷),科学出版社,中国北京,pp.104)
Fu L.G.,ed.,1991,China plant red data book:rare and endan gered plants,Science Press,Beijing,China,pp.1-10(傅立国主编,1991,中国植物红皮书-稀有濒危植物,科学出版社,中国,北京,pp.1-10)
Huang Y.H.,Ye J.H.,Dai Y.F.,Ren J.,Shun Q.S.,and Wei G.2014,Comparative study on pre-column derivation HPLCcharacteristic spectrum of polysaccharide from Dendrobium huoshanense and Dendrobium loddigesii,Zhongyao Xinyao Yu Linchuang Yaoli(Traditional Chinese Drug Research and Clinical Pharmacology),(6):725-730(黄月纯,叶家宏,戴亚峰,任晋,顺庆生,魏刚,2014,霍山石斛与美花石斛多糖柱前衍生HPLC特征图谱比较,中药新药与临床药理(6):725-730)
Li Z.Y.,and Xu L.,2014,Investigation of morphological charac teristics during liquid oscillation propagation of Dendrobium officinale,Jiyinzuxue Yu Yingyong Shengwuxue(Genomic and Applied Biology),33(2):382-385(李志英,徐立,2014铁皮石斛液体振荡培养的形态学观察,基因组学与应用生物学,33(2):382-385)
National pharmacopoeia commission,ed.,2015,Pharmacopoeia of people's republic of China,China Medical Science and Technology Press,Beijing,China,pp.92(国家药典委员会编著,2015,中华人民共和国药典,中国医药科技出版社中国,北京,pp.92)
Tong W.J.,Zhang L.,Xue Q.Y.,Hou B.W.,and Ding X.Y.2014,Isolation of endophytic bacteria in Dendrobium loddi gesii collected from different locations and comparison on their plant-growth-promoting potential,Zhiwu Ziyuan YHuanjing Xuebao(Journal of Plant Resources and Environ ment),23(1):16-23(童文君,张礼,薛庆云,侯北伟,丁小余2014,不同产地美花石斛内生细菌分离及促生潜力比较植物资源与环境学报,23(1):16-23)
Zhu H.,Teng J.B.,Cai Y.,Liang J.,Zhu Y.L.,and Wei T.,2011Relativity among starch quantity,polysaccharides conten and total alkaloid content of Dendrobium loddigesii,Zhong guo Zhongyao Zazhi(China Journal of Chinese Materia Me dica),36(23):3262-3264(朱华,滕建北,蔡毅,梁洁,朱意麟,韦韬,2011,美花石斛茎的淀粉粒数量,多糖含量与生物碱含量的相关性研究,中国中药杂志,36(23):3262-3264
基本信息:
DOI：10.13417/j.gab.034.002743
中图分类号:S567.239
引用信息:
[1]韦艳梅,谭小明,周雅琴.不同因素对濒危药用植物美花石斛壮苗的影响[J].基因组学与应用生物学,2015,34(12):2743-2747.DOI:10.13417/j.gab.034.002743.
基金信息:
广西科学基金(2015GXNSFDA139012)资助
2015-12-25
2015-12-25