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植酸酶是应用最广泛的饲料添加剂之一,它在自然界中广泛存在,能提高饲料中磷的利用率,对减轻动物高磷粪便导致的环境水域污染有着重要意义。玉米是动物饲料的主要原料,在玉米饲料中添加植酸酶易造成饲料成本升高和生产效率降低等问题。近年来随着基因工程技术的发展,已克隆出多个真菌植酸酶基因,并成功在微生物、植物系统中表达。本文主要介绍了植酸酶基因的微生物来源与相关研究成果,阐述了转基因技术的常用方法和转植酸酶基因玉米的研究进展,并详细评价了转植酸酶基因玉米的安全性。通过对转植酸酶基因玉米的深入探讨,对改善玉米的品质,降低成本及推广植酸酶在饲料工业中的应用具有重要意义。
Abstract:Phytase is one of most widespread feed additives,which exists widely in nature.It can enhance the phosphorus andmineral absorption in monogastric animals,and it is significant for reducing the level of phosphorus output in nature.Maize is the major ingredient of animal feed,and it may enhance cost or reduce efficiency to maize by adding phytase.Recently,with the development of gene engineering technique,many phytase genes from fungus have been cloned and successfully expressed in the microorganisms and plants.In this review,we mainly introduced the microorganism resource of phytase gene and the relative investigation achievements,expounded the methods of transgenic technology and the research progress of the transgenic maize plants expressing phytase gene.Further,we also evaluated the safety of phytase transgenic maize.According to this article,we hope that it would improve the quality of maize,reduce the cost of production and promote the application of phytase in feed industry in the future.
Chen R.M.,Xue G.X.,Chen P.,Yao B.,Yang W.Z.,Ma Q.L.,Fan Y.L.,Zhao Z.Y.,Tarczynski M.C.,and Shi J.R.,2008,Transgenic maize plants expressing a fungal phytase gene,Transgenic Res.,17(4):633-643
Chelius M.K.,and Wodzinski R.J.,1994,Strain improvement of Aspergillus niger for phytase production,Applied Microbiol-ogy Biotechnology,41:79-83
Christensen A.H.,Sharrock R.A.,and Quail P.H.,1992,Maize polyubiquintin genes:Structure,thermal perturbation of ex-pression and transcript splicing,and promoter activity fol-lowing transfer to protoplasts by electoporation,Plant Molecular Biology,18:675-689
Chen J.J.,Wang J.,Wan Y.S.,and Jin W.J.,2007,Safety assess-ment and commercialization of transgenic crops,Zhongguo Nongye Keji Daobao(Journal of Agricultural Science and Technology),9(3):38-43(陈洁君,王劲,宛煜嵩,金芜军,2007,转基因作物安全性评价与商品化前景分析,中国农业科技导报,9(3):38-43)
Chassy B.M.,2008,Food safety of crops and foods produced through biotechnology,Updated,8:1-5
DvorákováJ.,VolfováO.,and Kopeck伥J.,1997,Characteriza-tion of phytase produced by Aspergillus niger,Folia Micro-biologica,42(4):349-352
Dong Y.Z.,Duan S.J.,Zhao L.Y.,Yang X.H.and Jia S.R.,1999,Production of transgenic millet and maize plants by particle bombardment,Zhongguo Nongye Kexue(Scientia Agricul-tura Sinica),32(2):11-15,114(董云洲,段胜军,赵连元,杨秀海,贾士荣,1999,用基因枪转化花粉获得转基因谷子和玉米,中国农业科学,32(2):11-15,114)
Dong Q.Z.,Cheng B.J.,and Zhu S.W.,2006,Identification and inheritance analysis of transgenic maize by PFGE,Anhui Nongye Daxue Xuebao(Journal of Anhui Agricultural Uni-versity),33(1):21-24(董琼珠,程备久,朱苏文,2006,转基因玉米的脉冲电泳技术检测及遗传分析,安徽农业大学学报,33(1):21-24)
Fromm M.E.,Taylor L.P.,and Walbot V.,1986,Stable transfor-mation of maize after gene transfer by electroporation,Na-ture,319:791-793
Finamore A.,Roselli M.,Britti S.,Monastra G.,Ambra R.,Turri-ni A.,and Mengheri E.,2008,Intestinal and peripheral im-mune response to MON810maize ingestion in weaning and old mice,J.Agric.Food Chem.,56(23):11533-11539
Gao X.R.,Wang G.K.,Su Q.,Wang Y.,and An L.J.,2007,Phy-tase expression in transgenic soybeans:Stable transforma-tion with a vector-less construct,Biotechnology Letters,29(11):1781-1787
Gllovkin M.V.,Abraham M.,Morocz S.,Bottka S.,Feher A.,and Dudits D.,1993,Production of transgenic maize plants by direct DNA uptake into embryogenic protoplasts,Plant Sci.,90:41-52
Grant S.R.,1999,Dissecting the mechanisms of posttranscrip-tional gene silencing:Divide and conquer,Cell,96:303-306
Igbasan F.A.,Mnner K.,Miksch G.,Borriss R.,Farouk A.,and Simon O.,2000,Comparative studies on the in vitro proper-ties of phytase from various microbial origins,Arch.Tier-ernahr.,53(4):353-373
Kleter G.A.,and Kuiper H.A.,2002,Considerations for the as-sessment of the safety of genetically modified animals used for human food or animal feed,Livestock Production Sci-ence,74:275-285
Li G.L.,Yang S.H.,Li M.G.,Qiao Y.K.,and Wang J.H.,2009,Functional analysis of an Aspergillus ficuum phytase gene in Saccharomyces cerevisiae and its root-specific,secretory ex-pression in transgenic soybean plants,Biotechnology Let-ters,31(8):1297-1303
Liu X.F.,2003,Study on transformation of linearized phyAⅡgene into maize inbred lines by pollen-tube pathway,Thesis for M.S.,Liaoning Normal University,Supervisors:Hou H.S.,and An L.J.,pp.18(刘欣芳,2003,花粉管通道法将线性化植酸酶基因导入玉米自交系的研究,硕士学位论文,辽宁师范大学,导师:侯和胜,安利佳,pp.18)
Liu Q.Q.,Li Q.F.,Jiang L.,Zhang D.J.,Wang H.M.,Gu M.H.,and Yao Q.H.,2006,Transgenic expression of the recombi-nant phytase in rice(Oryza sativa),Rice Science,13(2):79-84
Liu X.,Zheng W.J.,Tang D.Z.,Zhao W.D.,He Y.,and Liu H.,2009,The research of genetically modified maize detection with gene chip,Shipin Yanjiu Yu Kaifa(Food Research and Development),6(30):149-153(刘烜,郑文杰,唐丹舟,赵卫东,贺艳,刘辉,2009,转基因玉米DNA检测芯片的研究,食品研究与开发,6(30):149-153)
Mandviwala T.N.,and Khire J.M.,2000,Production of high ac-tivity thermostable phytase from thermotolerant Aspergillus niger in solid state fermentation,Journal of Industrial Micro-biology&Biotechnology,24(4):237-243
Miksch G.,Kleist S.,Friehs K.,and Flaschel E.,2002,Overex-pression of the phytase from Escherichia coli and its extra-cellular production in bioreactors,Applied Microbiology and Biotechnology,59(6):685-694
Naqvi S.,Ramessar K.,Farre G.,Sabalza M.,Miralpeix B.,Twyman R.M.,Capell T.,Zhu C.F.,and Christou P.,2011,High-value products from transgenic maize,Biotechnology Advances,29:40-53
Onyango E.M.,Bedford M.R.,and Adeola O.,2005,Phytase ac-tivity along the digestive tract of the broiler chick:A com-parative study of an Escherichia coli-derived and Peniophora lycii phytase,Can.J.Anim.Sci.,85(1):61-68
Pu L.K.,2009,The extraction,purification and characterization of recombinant phytase from transgenic maize seeds,Thesis for M.S.,Lanzhou University,Supervisors:Pu X.,and Chen R.M.,pp.34-37(蒲凌奎,2009,转基因玉米种子中重组植酸酶的提取、纯化和酶学性质研究,硕士学位论文,兰州大学,导师:蒲训,陈茹梅,pp.34-37)
Shen Y.O.,Wang H.N.,and Pan G.T.,2008,Improving inorganic phosphorus content in maize seeds by introduction of phy-tase gene,Biotechnology,7:323-327
Scott M.P.,and Pollak L.M.,2005,Transgenic maize,Starch/St-rke,57:187-195
Shen Y.O.,2005,Studies on transformation of phytase gene(phy A)into maize,Thesis for M.S.,Sichuan Agricultural U-niversity,Supervisor:Pan G.T.,pp.5-6(沈亚欧,2005,植酸酶基因(phyA)转化玉米的研究,硕士学位论文,四川农业大学,导师:潘光堂,pp.5-6)
Ullah A.H.,1988,Aspergillus ficuum phytase:Primary structure,substrate selectivity and kinetic characterization,Prep.Biochem.,18(4):459-471
Wang Y.F.,2004,Study on transformation linearized phytase gene fragment into maize,Thesis for M.S.,Dalian Univer-sity of Technology,Supervisor:Luan Y.S.,pp.31(王永峰,2004,植酸酶基因线性化片段转化玉米的研究,硕士学位论文,大连理工大学,导师:栾雨时,pp.31)
Wu H.B.,Sun H.W.,Li B.D.,Yang C.L.,and Lu X.B.,2009,De tection of genetically modified maize by multiplex PCR-gene chip,Nongye Shengwu Jishu Xuebao(Journal of A-gricultural Biotechnology),17(6):1075-1082(武海斌,孙红炜,李宝笃,杨崇良,路兴波,2009,转基因玉米多重PCR-基因芯片联用的检测方法,农业生物技术学报,17(6):1075-1082)
Xu Z.Q.,Gong L.G.,Huang X.,Zhang Y.Y.,and Gao L.M.,2004,Transgenic maize plants with low copy number of for-eign genes were produced with maize Ubi-1promoter,Shengwu Gongcheng Xuebao(Chinese Journal of Biotech-nology),20(1):120-125(徐子勤,龚莉桂,黄萱,张永彦,高丽美,2004,采用玉米Ubi-1启动子获得低拷贝转基因玉米植株,生物工程学报,20(1):120-125)
Yu X.F.,2003,Construction of maize recombinantion expression vector with phyA gene encoding region from an aspergillus niger N25,Thesis for M.S.,Sichuan Agriculture University,Supervisor:Wang H.N.,pp.20(于新芬,2003,黑曲霉N25植酸酶phyA基因玉米表达载体的构建,硕士学位论文,四川农业大学,导师:王红宁,pp.20)
Yao B.,and Fan Y.L.,2000,Molecular biology and gene engi-neering of phytase,Shengwu Gongcheng Xuebao(Chinese Journal of Biotechnology),16(1):5-9(姚斌,范云六,2000,植酸酶的分子生物学与基因工程,生物工程学报,16(1):5-9)
Yang W.Z.,Pu L.K.,Zhang Q.,Chen P.,Chen R.M.,and Fan Y.L.,2008,Stability of phytase in transgenic corn in simulated gastric/intestinal fluid,Zhongguo Nongye Keji Daobao(Journal of Agricultural Science and Technology),10(S1):86-89(杨文竹,蒲凌奎,张琪,陈平,陈茹梅,范云六,2008,转植酸酶基因玉米中植酸酶蛋白在模拟消化液中的稳定性研究,中国农业科技导报,10(S1):86-89)
Zhang Q.,Chen R.M.,Yang W.Z.,Chen P.,Xue G.X.,and Fan Y.L.,2010,The obtaining of transgenic maize plants with PhyA2gene constitutive express phytase,Nongye Shengwu Jishu Xuebao(Journal of Agricultural Biotechnology),18(4):623-629(张琪,陈茹梅,杨文竹,陈平,薛光行,范云六,2010,组成型表达转植酸酶基因(phyA2)玉米的获得,农业生物技术学报,18(4):623-629)
Zou L.K.,2004,Expression of Bacillus subtilis WHNB02phytase phyC gene in E.coli and the construction of maize expres-sion vector,Thesis for M.S.,Sichuan Agriculture University,Supervisor:Wang H.N.,pp.29-30(邹立扣,2004,枯草芽孢杆菌WHNB02植酸酶phyC基因在大肠杆菌中的表达及玉米表达载体的构建,硕士学位论文,四川农业大学,导师:王红宁,pp.29-30)
基本信息:
中图分类号:S513;Q943.2
引用信息:
[1]熊建文,彭端,覃晓娟,等.转植酸酶基因玉米的研究与安全评价[J].基因组学与应用生物学,2011,30(02):251-256.
2011-04-28
2011-04-28