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本研究探讨了还原剂β-巯基乙醇(β-ME)、二硫苏糖醇(DTT)及谷胱甘肽(GSH)预处理水牛精子对水牛ICSI介导转基因(ICSI-mediated gene transfer,ICSI-Tr)效果的影响。结果显示,β-ME、DTT和GSH预处理水牛精子对提高ICSI介导转基因的效果不显著(p>0.05),但GSH能显著提高2原核(PN)的形成率(p<0.05)。
Abstract:Effects of sperm pretreatment with reducing agents(β-mercaptoethanol, β-ME; Dithiothreitol, DTT;Glutathione, GSH) on efficiency of ICSI-Tr in water buffalo were investigated. The results showed that, the sperm pretreatment of the three reducing agents could not improve the efficiency of ICSI-Tr in buffalo(p>0.05). But the pretreatment of GSH could significantly improve the 2PN formation rat(p<0.05).
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基本信息:
DOI:10.13417/j.gab.033.000537
中图分类号:S823.83
引用信息:
[1]陈自洪,孟凡丽,王丹,等.不同还原剂预处理精子对水牛ICSI介导转基因效果影响的初步研究[J].基因组学与应用生物学,2014,33(03):537-542.DOI:10.13417/j.gab.033.000537.
基金信息:
国家高技术研究发展计划(863)项目(2011AA100607);; 国家转基因重大专项(2011ZX08007-003)共同资助
2014-06-28
2014-06-28