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以‘丰香’草莓为试材,通过同源克隆法获得FaHY5 cDNA全长序列,运用生物学软件对该序列及其编码的氨基酸序列进行相关生物信息学及结构功能分析,同时采用半定量及荧光定量方法对FaHY5基因在不同组织和果实不同发育阶段的表达模式进行研究。结果表明,FaHY5全长为501 bp,Gen Bank登录号为KP984791,编码166个氨基酸。蛋白质分子量约为18.136 kD,理论等电点为10.05,C末端存在典型的bZIP保守结构域,是一个非分泌型核定位蛋白。序列比对及进化树分析,HY5在进化的过程中具有高度的保守性。表达谱分析,FaHY5在各组织中均有表达,且在花中表达量最高;而在果实发育过程中,FaHY5主要在前期积累。本研究分离鉴定了草莓中HY5(LONG HYPOCOTYL 5)转录因子并探索了其在草莓中的表达模式,为进一步研究草莓FaHY5转录因子的功能和作用机制提供理论基础。
Abstract：Fa HY5 gene was cloned from strawberry(Fragaria×ananassa cv. Toyonaka) though homology cloning method. Then, Bioinformatics and expression pattern of Fa HY5 were analysed by some softwares and PCR methods, respectively. The full length of Fa HY5 c DNA is 501 bp and Gen Bank accession number is KP984791. It encodes a protein consist of 166 amino acids residues with a molecular mass of 18.136 KD and a p I of 10.05. The protein contains the b ZIP conserved domain, which was located in nucleus and has no signal peptide. Sequence alignment and phylogenetic analysis, HY5 is highly conserved in evolution process. Gene expression profiling,Fa HY5 expresses among all different tissues, and the highest expression level is detected in flower. During fruit development, Fa HY5 accumulates mainly in the early stages. This research isolated and identificated light responsive b ZIP Transcription Factor HY5(LONG HYPOCOTYL 5) from strawberry and analysed gene expression pattern, which may provide a theoretical basis for further study of the function and molecular regulation mechanism.
Ang L.H.,Ch atto padhyay S.,Wei N.,Oyama T.,Okada K.Batschauer A.,and Deng X.W.,1998,Molecular interaction between COP1 and HY5 defines a regulatory switch fo light control of Arabidopsis development,Molecular Cell,1(2):213-222
Azuma A.,Fujii H.,Shimada T.,and Yakushiji H.,2015,Mic roarray analysis for the screening of genes inducible by ligh or low temperature in post-veraison grape berries,The Hor ticulture Journal,84(3):214-226
Catala R.Medina J.,and Salinas J.,2011,Integration of low temper ature and light signaling during cold acclimation response in Arabidopsis,Proc.Natl.Acad.Sci.USA,108(39):16475-16480
Chen H.,Zhang J.,Neff M.M.,Hong S.W.,Zhang H.Y.,Deng XW.,and Xiong L.,2008,Integration of light and abscisi acid signaling during seed germination and early seedling development,Proc.Natl.Acad.Sci.USA,105(11):4495-4500
Chen Q.,Yu H.W.,Wang X.R.,Xie X.L.,Yue X.Y.,and Tang H.R2012,An alternative cetyltrimethylammonium bromide-based protocol for RNA isolation from blackberry(Rubus L.),Ge netics and Molecular Research,11(2):1773-1782
Christie J.M.,Arvai A.S.,Baxter K.J.,Heilmann M.,Pratt A.J.O’Hara A.,Kelly S.M.,Hothorn M.,Smith B.O.,Hitomi K.Jenkins G.I.,and Getzoff E.D.,2012,Plant UVR8 photore ceptor senses UV-B by tryptophan-mediated disruption o cross-dimer salt bridges,Science,335(6075):1492-1496
Cluis C.P.,Mouchel C.F.,and Hardtke C.S.,2004,The Ara bidopsis transcription factor HY5 integrates light and hor mone signaling pathways,The Plant Journal,38(2):332-347
Franklin K.A.,Toledo-Ortiz G.,Pyott D.E.,and Halliday K.J.2014,Interaction of light and temperature signaling,Journa of Experimental Botany,65(11):2859-2871
Hardtke C.S.,Gohda K.,Osterlund M.T.,Oyama T.,Okada K.Okada K.,and Deng X.W.,2000,HY5 stability and activit in arabidopsis is regulated by phosphorylation in its COP1binding domain,The EMBO Journal,19(18):4997-5006
Hou Y.X.,Tang H.R.,Zhang Y.,Luo Y.,and Chen Q.,2009Cloning and expression analysis of ascorbate peroxidas gene during fruit development and ripening in Fragaria×ananassa cv.Toyonaka,World Journal of Agricultural Sci ence,5(6):675-679
Jakoby M.,Weisshaar B.,DR魻GE-Laser W.,Vicente-Carbajos J.,Tiedemann J.,Kroj T.,and Parcy F.,2002,b ZIP tran scription factors in Arabidopsis,Trends in Plant Science7(3):106-111
Jiao Y.,Lau O.S.,and Deng X.W.,2007,Light-regulated tran scriptional networks in higher plants,Nature Reviews Ge netics,8(3):217-230
Lau O.S.,and Deng X.W.,2010,Plant hormone signaling light ens up:integrators of light and hormones,Current Opinion in Plant Biology,13(5):571-577
Li H.F.,Wang X.F.,Ran K.,He P.,Wang H.B.,and Li L.G.,2014Expression and protein interaction analysis of light respon sive b ZIP transcription factor Md HY5,Zhongguo Nongy Kexue(Scientia Agricultura Sinica),47(21):4318-4327(李慧峰,王小非,冉昆,何平,王海波,李林光,2014,苹果光响应转录因子Md HY5表达及蛋白互作分析,中国农业科学,47(21):4318-4327)
Oyama T.,Shimura Y.,and Okada K.,1997,The Arabidopsi HY5 gene encodes a b ZIP protein that regulates stimu lus-induced development of root and hypocotyl,Genes&Development,11(22):2983-2995
Rizzini L.,Favory J.J.,Cloix C.,Faggionato D.,O'Hara A.Kaiserli E.,Baumeister R.,Sch覿fer E.,Nagy F.,Jenkins G.I.and Ulm R.,2011,Perception of UV-B by the Arabidopsi UVR8 protein,Science,332(6025):103-106
Sun M.,Zhou B.,Wang Y.,and Li Y.H.,2009,Advance o researches on light regulator COP1,HY5 in plant,Shengwu Jishu Tongxun(Letters in Biotechnology),20(2):291-294(孙梅,周波,王宇,李玉花,2009,植物光调控因子COP1HY5的研究进展,生物技术通讯,20(2):291-294)
Ito S.,Song Y.H.,and Imaizumi T.,2012,LOV domain-contain ing f-box proteins:light-dependent protein degradation mod ules in Arabidopsis,Molecular Plant,5(3):573-582
Vandenbussche F.,Habricot Y.,Condiff A.S.,Maldiney R.,Van der Straetend D.,and Ahmad M.,2007,HY5 is a point o convergence between cryptochrome and cytokinin signalling pathways in Arabidopsis thaliana,The Plant Journal,49(3)428-441
Weller J.L.,Hecht V.,Vander-Schoor J.K.,Davidson S.E.,and Ross,J.J.,2009,Light regulation of gibberellin biosynthesi in Pea is mediated through the COP1/HY5 pathway,Th Plant Cell Online,21(3):800-813
Wu D.,Hu Q.,Yan Z.,Chen W.,Yan C.Y.,Huang X.,Zhang J.Yang P.,Deng H.,Wang J.,Deng X.,and Shi Y.,2012Structural basis of ultraviolet-B perception by UVR8,Na ture,484(7393):214-219
Yang L.Y.,Li J.Y.,Wang L.T.,Duan S.Z.,Ma J.H.,Ma E.D.and Gong M.,2015,Research advances about effects o light environment on growth and material metabolism of to bacco(Nicotiana tabacum L.)plants,Jiyinzuxue Yu Yingy ong Shengwuxue(Genomics and Applied Biology),34(5)1114-1128(杨利云,李军营,王丽特,段胜智,马俊红,马二登,龚明,2015,光环境对烟草生长及物质代谢的影响研究进展,基因组学与应用生物学,34(5):1114-1128)
Yang Y.,Gao S.Q.,Tang Y.M.,Ye X.F.,Wang Y.B.,Liu Y.M.and Zhao C.P.,2009,Advance of b ZIP transcription factor in plant,Mailei Zuowu Xuebao(Journal of Triticeae Crops)29(4):730-737(杨颖,高世庆,唐益苗,冶晓芳,王永波,刘美英,赵昌平,2009,植物b ZIP转录因子的研究进展,麦类作物学报,29(4):730-737)
Yang Y.X.,Wang F.,Cai J.X.,Yu J.Q.,and Zhou Y.H.,2014Recent advances in the role of light quality and phy tochrome in plant defense resistance against environmenta stresses,Yuanyi Xuebao(Acta Horticulturae Sinica),41(9)1861-1872(杨有新,王峰,蔡加星,喻景权,周艳虹,2014光质和光敏色素在植物逆境响应中的作用研究进展,园艺学报,41(9):1861-1872)
Yoon M.K.,Shin J.,Choi G.,and Choi B.S.,2006,Intrinsicall unstructured N-terminal domain of b ZIP transcription facto HY5,Proteins:Structure,Function,and Bioinformatics,65(4):856-866
Yu Y.W.,Wang J.,Zhang Z.J.,Quan R.D.,Zhang H.W.,Deng XW.,Ma L.,and Huang R.,2013,Ethylene promotes hypo cotyl growth and HY5 degradation by enhancing the move ment of COP1 to the nucleus in the light,PLo S Genetics,9(12):e1004025
Zhang L.,Zhou B.,and Li Y.H.,2010,Advances in the structur and function of HY5 in plant,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),46(10):985-990(张荔周波,李玉花,2010,植物HY5蛋白结构与功能的研究进展,植物生理学通讯,46(10):985-990)
Zhou B.,Wang Y.,Sun M.,and Li Y.H.,2008,c DNA cloning of b ZIP protein HY5 in Tsuda Turnip and its transcript expression analysis,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),6(1):59-64(周波,王宇,孙梅,李玉花,2008,津田芜菁b ZIP蛋白HY5 c DNA的克隆及表达特性,分子植物育种,6(1):59-64)
基本信息:
DOI：10.13417/j.gab.034.002695
中图分类号:S668.4;Q943.2
引用信息:
[1]张云婷,陈品文,江雷雨,等.草莓光信号转导因子FaHY5的克隆及其表达分析[J].基因组学与应用生物学,2015,34(12):2695-2701.DOI:10.13417/j.gab.034.002695.
基金信息:
高等学校博士学科点专项科研基金(20125103110005)资助
2015-12-25
2015-12-25