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本研究从农药污染土样中筛选出1株具有敌百虫降解特性的菌株Lin F-1,通过显微结构观察,生理生化测试,16S rDNA序列测定和同源性分析,初步鉴定为Streptomyces levis,属于链霉菌属。对该菌的农药降解特性研究表明,Lin F-1对有机磷、有机氯以及菊酯类等农药具有较好的广谱降解能力。在敌百虫浓度为100 mg/L,温度为28℃条件下培养1周,Lin F-1对其降解动力曲线满足降解一级动力学方程ln(Ct/Co)=-1.732 9t+1.893,其最高降解率达到63.3%,敌百虫浓度半数降解时间(DT50)为4.41 d。在基础培养基中,Lin F-1的敌百虫最适降解浓度为100 mg/L,同时在加入5种外源碳源的条件下,Lin F-1的生物量和敌百虫降解率均有显著性提高,最高分别达到了106.5 mg/mL和97.7%。
Abstract：One strain Lin F-1,with highly trichlorfon biodegradation ability,was isolated from the pesticide polluted soil samples,and was firstly identified as Streptomyces levis by the analysis of micro-structure,physiology and biochemistry tests,the 16 S rDNA sequences and homology analysis.The broad-spectrum pesticide degradation test also proved the preferable degradation abilities of Lin F-1 in organophosphorus,organochlorine and pyrethroid pesticides.Lin F-1 was inoculated in the medium containing 100 mg/L trichlorfon at 28℃ for one week,and the degradation of power curve was measured by the first-order kinetic model as followed:ln(Ct/Co)=-1.732 9t+1.893.The highest degradation rate,which was 63.3%,was measured after one week culturing,and the time that half of the pesticide concentration was degraded as 4.41 days.The optimum trichlorfon concentration in the mineral medium was determined as 100 mg/L.The dry weight and trichlorfon degradation rate were significantly improved with adding five kinds of different exogenous carbon sources,which reached the highest of 106.5 mg/mL and 97.7%,respectively.
Ahmed R.Z.,and Ahmed N.,2014,Effect of yeast extract on fluoranthene degradation and aromatic ring dioxygenase expressing bacterial community structure of a fluoranthene degrading bacterial consortium,Int.Biodeter.Biodegr.,88(1):56-61
Awad N.S.,Sabit H.H.,Abo-Aba S.E.M.,and Bayoumi R.A.,2011,Isolation,characterization and fingerprinting of some chlorpyrifos-degrading bacterial strains isolated from Egyptian pesticides-polluted soils,Aft.J.Microbiol.Res.,5(18):2855-2862
Benvenuti M.,Briganti F.,Scozzafava A.,Golovleva L.,Travkin V.M.,and Mangani S.,1999,Crystallization and preliminary crystallographic analysis of the hydroxyquinol 1,2-dioxygenase from nocardioides simplex 3E:a novel dioxygenase involved in the biodegradation of polychlorinated aromatic compounds,Acta Crystallogr.D.,55(4):901-903
Cycon M.,Zmijowska A.,and Piotrowska-Seget Z.,2011,Biodegradation kinetics of 2,4-D by bacterial strains isolated from soil,Cent.Eur.J.Bio1,6(2):188-198
Deshpande N.M.,Samaik S.S.,Paranjpe S.A.,and Kanekar P.P.,2004,Optimization of dimethoate degradation by brevundimonas sp.MCM B-427 using factorial design:studies on interactive effects of environmental factors,World J.Microb Biot.,20(5):455-462
Fan Y.X.,Chen X.L.,Jiang X.P.,and Wang Z.,2002,Advance in methamidophos biodegradation study,Weishengwuxue Zazhi(Journal Of Microbiology),22(3):45-47(范永仙,陈小龙,姜晓平,汪钊,2002,甲胺磷农药的生物降解研究进展,微生物学杂志,22(3):45-47)
Jauregui J.,Valderrama B.,Albores A.,and Vazquez-Duhalt R.,2003,Microsomal transformation of organophosphorus pesticides by white rot fungi,Biodegradation,14(6):397-406
Jiang J.D.,2006,Construction of multifunctional pesticides degrading genetically engineered microorganism and its risk assessment for environmental release,Dissertation for Ph.D.,College of Life Science,Nanjing Agricultural University,Supervisor:Li S.P.,pp.79-95(蒋建东,2006,多功能农药降解基因工程菌的构建及其环境释放安全评价研究,博士学位论文,南京农业大学生命科学学院,导师:李顺鹏,pp.79-95)
Jiang Y.J.,2006,Isolation of organophosphorous pesticides degrading bacterium and determination of the responsible enzyme's properties,Dissertation for Ph.D.,College of Plant Protection,Fujian Agriculture and Forestry University,Supervisor:Hu F.P,pp.80-92(江玉姬,2006,有机磷农药降解菌的筛选及其降解酶特性,博士学位论文,福建农林大学植物保护学院,导师:胡方平,pp.80-92)
Karpouzas D.G.,Hatziapostolou P.,Papadopoulou-Mourkidou E.,Giannakou I.O.,and Georgiadou A.,2004,The enhanced biodegradation of fenamiphos in soils from previously treated sites and the effect of soil fumigants,Environ.Toxicol.Chem.,23(9):2099-2107
Li R.,Jia K.Z.,Jiang J.D.,Shen X.Q.,Pan R.Q.,and Li S.P.,2007,Isolation and identification of a bacterium DDB-1capable of degrading dichlorvos and trichlorfon simultaneously and its degrading characteristics,Nongye Huanjing Kexue Xuebao(Journal of Agro-environment Science),26(2):554-558(李荣,贾开志,蒋建东,沈小琴,潘荣清,李顺鹏,2007,敌敌畏,敌百虫高效降解菌株DDB-1的分离鉴定及降解特性研究,农业环境科学学报,26(2):554-558)
Liu L.,Zhang Y.J.,Xu C.Z.,and Luo F.,2014,A modified CTAB method for isolating genome DNA from fungus with abundant polysaccharose,Zhongguo Shengwu Gongcheng Zazhi(China Biotechbology),34(5):75-79(刘丽,张永军,许长征,罗锋,2014,一种改良的CTAB法提取产多糖真菌DNA,中国生物工程杂志,34(5):75-79)
Mataqueiro M.I.,Nakaghi L.S.O.,Yamada P.K.,de Camargo Ferraz G.,de Queiroz-Neto A.,and De Oliveira G.H.,2014,Degradation,residual determination,and cholinesterase activity of triclorfon in piaractus mesopotamicus holmberg(PACU)1887,J.Toxicol.Env.Health,77(1-3):125-132
Pu L.,You L.,He Q.,and Wang T.,2011,Adaptability of 8streptomyces sp.strains isolated from mud of pit wall to acid and alcohol,Zhongguo Niangzao(China Brewing),30(7):61-64(蒲岚,游玲,何琴,王涛,2011,8株窖泥链霉菌对酸、乙醇的适应性研究,中国酿造,30(7):61-64)
Tang M.Q.,2012,Study on the isolation and identification of a triazophos-degrading bacterium and the characterization of its degradative enzyme,Dissertation for Ph.D.,College of Plant Protection,Fujian Agriculture and Forestry University,Supervisor:You M.S.,pp.65-80(汤鸣强,2012,三唑磷降解菌的分离鉴定及其降解酶特性的研究,博士学位论文,福建农林大学植物保护学院,导师:尤民生,pp.65-80)
Wang R.,and Zeng X.Y.,2012,Character of organophosphorus pesticide trichlorphon-degrading bacterial strain multiplexed system,Shengwu Jishu Shijie(Biotechnology World),(4):6-7(王锐,曾祥羽,2012,有机磷农药敌百虫多菌株复合系降解菌的降解特性研究,生物技术世界,(4):6-7)
Yang Y.,Deng J.,Zhang L.,Jiang C.J.,On Q.,Shen P.H.,and Wu B.,2015,Identification of a Klebsiella pneumoniae sp.GXW-1 which produced lysine decarboxylase,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(12):2639-2643(杨颖,邓洁,张亮,蒋承建,欧情,申佩弘,武波,2015,一株产赖氨酸脱羧酶的菌株Klebsiella pneumoniae sp.GXW-1的鉴定,基因组学与应用生物学,34(12):2639-2643)
Yu Y.L.,Song K.M.,Zheng Z.,Chen H.X.,and Fan D.F.,1997,Isolation and identification of a broad spectrum bacterial strain(Alcaligenes sp.)degrading pesticides,Zhejiang Nongye Daxue Xuebao(Journal of Zhejiang Agricultural University),23(2):111-115(虞云龙,宋凤鸣,郑重,陈鹤鑫,樊德方,1997,一株广谱性农药降解菌(Alcaligenes sp.)的分离与鉴定,浙江农业大学学报,23(2):111-115)
Zhu H.Z.,2008,Study on extraction,separation and detection of trichlorfon residue and capillary electrophoresis separation and detection of heavy metal,Dissertation for Ph.D.,College of Food Science And Technology,Nanjing Agricultural University,Supervisor:Yang M.M.,pp.13-18(祝海珍,2008,敌百虫残留的萃取、分离与检测的研究和重金属离子的芯片毛细管电泳分离检测,博士学位论文,南京农业大学食品科学技术学院,导师:杨明敏,pp.13-18)
基本信息:
DOI：10.13417/j.gab.035.001451
中图分类号:X592;X172
引用信息:
[1]余臣磊,田江,邓松强,等.一株广谱农药降解放线菌对敌百虫的降解特性研究[J].基因组学与应用生物学,2016,35(06):1451-1457.DOI:10.13417/j.gab.035.001451.
基金信息:
中央高校基本科研业务费专项资金(项目编号:2042014kf0234);; 微生物-植物强化修复工业场地农药污染土壤基金(项目编号:230100027)共同资助
2016-06-29
2016-06-29
2016-06-29