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γ-谷氨酰转肽酶是微生物体内一种结构特殊的异源二聚体酶,该酶能够将γ-谷氨酰基转移到其他氨基酸或肽类物质相关基团,它具有特殊的转肽、自转肽和水解三种催化反应。本研究综述通过吸附法、包埋法、交联法和共价结合法对γ-谷氨酰转肽酶进行固定化。该酶通过定点突变提高活性。它合成γ-谷氨酰基衍生物具有重要工业化应用前景。
Abstract:Gamma glutamyltranspe ptidase i s a heterodimer enzyme with special structure in microorganism. It could transfer gamma pancreatic acyl to other amino acids or peptides related groups. GGT has three catalytic reactions including turn peptide, rotation peptide and hydrolysis. In this review, GGT were immobilized by adsorption method, embedding method, crosslinking method and covalent bonding. It would improve activity by Site-directed mutagenesis techniques. Gamma glutamine derivatives synthesized by gamma glutamyltranspeptidase might have an important industrial application prospect.
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基本信息:
DOI:10.13417/j.gab.037.002438
中图分类号:Q814.2
引用信息:
[1]徐礼生,王梦婷,高贵珍,等.酶固定化技术及其在γ-谷氨酰转肽酶中的应用[J].基因组学与应用生物学,2018,37(06):2438-2442.DOI:10.13417/j.gab.037.002438.
基金信息:
安徽省高校自然科学研究重点项目(KJ2017A440);; 安徽省质量工程项目(2015zjjh034;2015ckjh108;2016jyxm1038);; 宿州区域发展协同创新中心(特色种植业方向)开放课题(2015SZXTZXKFYB01);; 宿州学院科研平台开放课题(2015YKF05)共同资助
2018-06-25
2018-06-25