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以黑木耳为原料,分别采用普通机械粉碎法和超微粉碎法对黑木耳进行粉碎处理,以黑木耳多糖的得率为指标,探究不同粒度对黑木耳多糖提取的影响,并通过其对OH·、DPPH·以及ABTS的清除能力来研究不同粒度得到的多糖的抗氧化活性。研究表明在最佳的提取条件下,不同粒度提取出的黑木耳多糖得率不同:60目以下、60目、120目和超微粉碎下的多糖得率分别为3.51%、5.25%、8.25%、9.72%,原料粒度越小,得率越高,且超微粉碎下用时仅为其他粒度的1/4;抗氧化活性实验表明,原料粒度越小,其抗氧化活性越高,其中对DPPH自由基清除效果最好,可达到86.96%,对ABTS的清除能力相对较差,通过与抗坏血酸阳性对照发现,不同粒度下的抗氧化性均低于抗坏血酸。
Abstract：In this paper, Auricularia auricula as raw material, which was ground by ordinary machine and superfine comminution, respectively, explored the effect of different particle sizes on the extraction of A. auricula polysaccharide, and studied on its antioxidant effect. With the A. auricula polysaccharide yield as the index, the effect of different particle sizes on the extraction of A. auricula polysaccharide and the antioxidant activities, such as the scavenging abilities of OH·, DPPH·, ABTS was explored. The results showed that under the optimum conditions of ultrasonic assisted extraction of polysaccharide from A. auricula, the yield of A. auricula polysaccharides extracted from different particle sizes was different. Polysaccharide yield of Auricularia auricula below60 mesh, 60 mesh, 120 mesh and superfine comminution were 3.51%, 5.25%, 8.25% and 9.72%, respectively. The smaller particle size of the raw material, the higher yield of A. auricula polysaccharide. And for the extraction time, superfine comminution is only 1/4 of other granularities. Antioxidant activities showed that the smaller particle size, the higher antioxidant activities. Among them, the scavenging effect of DPPH radical was the best, it could reach 86.96%, and the scavenging ability of ABTS was relatively poor, and the antioxidant properties were lower than that of ascorbic acid under different particle sizes by positive control with ascorbic acid.
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基本信息:
DOI：10.13417/j.gab.039.002183
中图分类号:R151
引用信息:
[1]鲍妮娜,骆晗,吕滔,等.探究不同粒度对黑木耳多糖的提取及其抗氧化活性的影响[J].基因组学与应用生物学,2020,39(05):2183-2190.DOI:10.13417/j.gab.039.002183.
基金信息:
宿州学院教授(博士)科研启动基金资助项目(2015JB09);; 宿州学院药物生物技术研究所开放课题(2015-YKF04,2017YKF05);; 大学生创新创业计划项目(201710379091)共同资助
2020-05-25
2020-05-25