| 374 | 7 | 85 |
| 下载次数 | 被引频次 | 阅读次数 |
欧拉羊(Oula sheep)属于藏羊(Tibetan sheep)的一个分支。不同于其他国内外绵羊(Ovis aries)品种，欧拉羊的公母羊皆生有大犄角。这是长期放牧条件下自然选择的结果，但其大犄角不利于现代高效畜牧业生产需求。已有较多研究把绵羊无角性状的主效控制基因定位于绵羊10号染色体上的RXFP2基因，但RXFP2基因在不同绵羊品种中的调控作用仍有差别。为加快青藏高原地区无角绵羊品种培育，本研究对青海有角型、无角型欧拉羊成年母羊各15只进行了10×深度的基因组重测序，采用FST选择性消除定位角表型主要选择基因，并对欧拉羊RXFP2基因CDS区域及上下50 kbp区域以2 000 bp窗口、 1 000 bp步长进行扫描，对获得的单核苷酸多态性(single nucleotide polymorphism, SNP)进行Tajima′s D、π、FST选择分析。结果发现，欧拉羊RXFP2基因及3′下游区域存在强选择信号。通过对RXFP2基因编码区SNP筛选，挖掘了欧拉羊中4个高质量SNPs,分别是位于RXFP2基因第9、第14外显子的同义突变(g.29458690A>G, g.29446067T>C)和第17外显子的2个非同义突变(g.29439053C>T, g.29439011C>T);突变区域均处于无角、有角欧拉羊RXFP2基因的强选择区域。本研究结果可为无角欧拉羊品种(品系)培育相关分子辅助选择工作提供技术和理论依据，有助于加快无角欧拉羊品种培育的早期选种工作。
Abstract：Oula sheep is one branch of Tibetan sheep, which is different from other domestic and foreign breeds of sheep(Ovis aries). Both male and female sheep are born with large horns, which is the result of natural selection under long-term grazing conditions, but its large horns are not conducive to modern efficient animal husbandry production needs. Many previous studies have mapped the main control gene of sheep hornless trait to RXFP2 gene on sheep chromosome 10, but the regulatory effect of RXFP2 gene in different sheep breeds is still different. In order to speed up the breeding of polled sheep breeds in the Qinghai-Tibet Plateau, 15 adult horned ewe and 15 adult hornless ewe Oula sheep(Ovis aries) were detected by genome resequencing, respectively, and FST was used to selectively eliminate the main selection genes for the angular phenotype. The CDS region and the upper and lower 50 kbp regions of RXFP2 gene of Oula sheep were scanned by 2 000 bp window and 1 000 bp step. Tajima′s D, π, and FST selection analysis were performed on the single nucleotide polymorphism(SNP) obtained. The results showed that there were strong selective signals in the RXFP2 gene and its downstream regions in Oula sheep. Four high quality SNP in the RXFP2 gene of Oula sheep were identified by SNP screening, which were synonymous mutations(g.29458690A>G, g.29446067T>C) in exon 9 and exon 14 of RXFP2 gene, and two non-synonymous mutations(g.29439053C>T, g.29439011C>T) in exons 17 of RXFP2 gene. All the mutant regions were in the strong selection region of RXFP2 gene of hornless and horned Oula sheep. The results of this study can provide technical and theoretical basis for molecular assisted selection related to the breeding of hornless Oula sheep breeds(strains), and help speed up the early breeding of hornless Oula sheep breeds.
ASHENAFI GETACHEW M,2016.RXFP2基因在青藏高原地区不同绵羊品种中的多态性研究，硕士学位论文.兰州：中国农业科学院研究生院，兰州畜牧与兽药研究所.(ASHENAFI GETACHEW M,2016.Polymorphism of RXFP2 gene in different sheep breeds in Qinghai-Tibet Plateau,Thesis for M.S.Lanzhou:Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Granduate school of Chinese Academy of Agricultural Sciences.)
程红，2021.外源基因渗入对绵羊基因组及表型的影响研究，博士学位论文.杨凌：西北农林科技大学.(CHENG H,2021.Effects of alien introgression on genome and phenoty-pic variation in sheep,Dissertation for Ph.D.Yangling:Northwest A & F University.)
李晨，2019.无角牦牛降生高原.中国科学报，2019-07-12(001)[2022-02-10].[LI C,2019.Hornless yak born on plateau.China Science Daily,2019-07-12(001).[2022-02-10]]
刘梦，阎明毅，余忠祥，等，2022.补饲对无角欧拉羊屠宰性能的影响.中国草食动物科学，42(5):75-79.[LIU M,YAN M Y,YU Z X,et al.,2022.Effects of supplementary feeding on slaughter performance of hornless Oula sheep.China Herbivore Science,42(5):75-79.]
王维民，2018.基于全基因组选择信号挖掘中国地方绵羊在驯化过程中的选择印记，博士学位论文.武汉：华中农业大学.[WANG W M,2018.Genome-wide detection of selection signatures reveals selective imprints of Chinese indigenous sheep breeds during domestication,Dissertation for Ph.D.Wuhan:Huazhong Agricultural University.]
王禹，2019.反刍动物角起源进化的比较基因组研究，博士学位论文.杨凌：西北农林科技大学.[WANG Y,2019.Comparative genomics analysis of origin and evolution of ruminant headgear,Dissertation for Ph.D.Yangling:Northwest A & F University.]
庄蕾，刘梦，阎明毅，等，2022.欧拉羊生长曲线拟合分析.黑龙江畜牧兽医 (10):34-39.[ZHUANG L,LIU M,YAN M Y,et al.,2022.Fitting analysis of growth curve of Oula sheep.Heilongjiang Animal Science and Veterinary Medicine (10):34-39.]
CAO Y H,XU S S,SHEN M,et al.,2021.Historical introgression from wild relatives enhanced climatic adaptation and resistance to pneumonia in sheep.Mol.Biol.Evol.,38(3):838-855.
DONIZETTI A,FIENGO M,DEL GAUDIO R,et al.,2015.Expression pattern of zebrafish rxfp2 homologue genes during embryonic development.J.Exp.Zool.B Mol.Dev.Evol.,324(7):605-613.
DUIJVESTEIJN N,BOLORMAA S,DAETWYLER H D,et al.,2018.Genomic prediction of the polled and horned phenotypes in Merino sheep.Genet.Sel.Evol.,50(1):28.
FENG S,FERLIN A,TRUONG A,et al.,2009.INSL3/RXFP2 signaling in testicular descent:mice and men.Ann.N.Y.Acad.Sci.,1160(1):197-204.
HARRIS R M,FINLAYSON C,WEISS J,et al.,2010.A missense mutation in LRR8 of RXFP2 is associated with cryptorchidism.Mamm.Genome,21(9):442-449.
HE X H,ZHOU Z K,PU Y B,et al.,2016.Mapping the four-horned locus and testing the polled locus in three Chinese sheep breeds.Anim.Genet.,47(5):623-627.
HU X J,YANG J,XIE X L,et al.,2019.The genome landscape of Tibetan sheep reveals adaptive introgression from argali and the history of early human settlements on the Qinghai-Tibetan plateau.Mol.Biol.Evol.,36(2):283-303.
KARDOS M,LUIKART G,BUNCH R,et al.,2015.Whole-genome resequencing uncovers molecular signatures of natural and sexual selection in wild Bighorn sheep.Mol.Ecol.,24(22):5616-5632.
KIJAS J W,LENSTRA J A,HAYES B,et al.,2012.Genome-wide analysis of the world′s sheep breeds reveals high levels of historic mixture and strong recent selection.PLoS Biol.,10(2):e1001258.
LI X,YANG J,SHEN M,et al.,2020.Whole-genome resequencing of wild and domestic sheep identifies genes asso-ciated with morphological and agronomic traits.Nat.Commun.,11(1):2815.
LüHKEN G,KREBS S,ROTHAMMER S,et al.,2016.The 1.78 kb insertion in the 3′-untranslated region of RXFP2 does not segregate with horn status in sheep breeds with variable horn status.Genet.Sel.Evol.,48(1):78.
PAN Z Y,LI S D,LIU Q Y,et al.,2018.Whole-genome sequences of 89 Chinese sheep suggest role of RXFP2 in the development of unique horn phenotype as response to semi-feralization.GigaScience,7(4):giy019.
SEROUSSI E,ROSOV A,SHIRAK A,et al.,2017.Unveiling genomic regions that underlie differences between Afec-Assaf sheep and its parental Awassi breed.Genet.Sel.Evol.,49(1):19.
WANG X L,ZHOU G X,LI Q,et al.,2014.Discovery of SNPs in RXFP2 related to horn types in sheep.Small Rumin.Res.,116(2-3):133-136.
WANG Y,ZHANG C Z,WANG N N,et al.,2019.Genetic basis of ruminant headgear and rapid antler regeneration.Science,364(6446):eaav6335.
WIEDEMAR N,DR?GEMüLLER C,2015.A 1.8-kb insertion in the 3′-UTR of RXFP2 is associated with polledness in sheep.Anim.Genet.,46(4):457-461.
XU S S,GAO L,SHEN M,et al.,2021.Whole-genome selective scans detect genes associated with important phenotypic traits in sheep (Ovis aries).Front.Genet.,12:738879.
ZHANG Y Y,XUE X L,LIU Y E,et al.,2021.Genome-wide comparative analyses reveal selection signatures underlying adaptation and production in Tibetan and Poll Dorset sheep.Sci.Rep.,11:2466.
基本信息:
DOI：10.13417/j.gab.043.000442
中图分类号:S826
引用信息:
[1]张强龙,吴森,阎明毅,等.青海欧拉羊(Ovis aries)群体中角发育控制基因RXFP2的选择信号分析[J].基因组学与应用生物学,2024,43(03):442-452.DOI:10.13417/j.gab.043.000442.
基金信息:
青海省重大科技专项(2021-NK-A5); 青海省畜牧兽医科学院基本科研业务费自主选题项目(MKY-2019-04); 农业生产发展资金-联合育种(羊)(2130122)共同资助
2023-12-07
2023-12-07
2023-12-07