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活化的蛋白激酶C受体1 (receptor for activated C kinase 1,RACK1)在动物胚胎发育以及细胞内多种激酶活性调节等过程中起着重要作用。为了进一步探讨不同物种RA CK1基因及其表达产物的特点,以昆虫拟黑多刺蚁为实验材料通过RT-PCR、基因克隆及测序等技术获得了RA CK1基因cDNA序列,并对该序列和表达产物的序列进行了生物信息学分析。研究发现,拟黑多刺蚁RA CK1基因cDNA长度为1 096 bp,其编码区序列(CDS)长度为954 bp编码317个氨基酸构成RACK1。拟黑多刺蚁RACK1蛋白中酸性氨基酸和碱性氨基酸数量分别是34和35,理论等电点为7.63。该蛋白与多个物种RACK1或G蛋白β亚基类似物是同源的具有典型的WD40蛋白家族结构域,其二级结构主要由β折叠和无规则卷曲构成,并由此形成螺旋桨状的高级结构。
Abstract：RACK1 plays an important role in the progress of embryonic development and regulating activity of various kinases in cells.To further understand the characteristics of RA CK1 gene and its expression product,a cDNA of RA CK1 gene from the ant Polyrhachis vicina was obtained by means of RT-PCR,gene cloning and sequencing.The sequences of the gene and its expression product were analyzed rely on different bioinformatics tools.The results show that RACK1 gene of Polyrhachis vicina was composed of 1 096 nucleotides,including a 954 bp CDS region which encoded a polypeptide of 317 amino acids.The RACK1 of Polyrhachis vicina which theoretical pI is 7.63 contains 34 acidic amino acids and 35 alkaline amino acids.This RACK1 protein with typical WD40 functional domain is homologous to that of other species which is named RACK1 or G β-like protein.Its secondary structure is mainly composed of strand and coil,which is the basic structure for propeller blade structure forming.
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基本信息:
DOI：10.13417/j.gab.039.004541
中图分类号:Q963
引用信息:
[1]郭新军.拟黑多刺蚁RACK1基因cDNA克隆及其生物信息学分析[J].基因组学与应用生物学,2020,39(10):4541-4546.DOI:10.13417/j.gab.039.004541.
基金信息:
陕西省自然科学基础研究计划项目(2020JM-619);; 陕西省教育厅专项科研计划项目(17JK1128);; 西安市科技计划项目(2016CXWL12)共同资助
2020-10-25
2020-10-25