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蛋白质磷酸化是生物体中广泛存在的翻译后修饰方式,参与多种过程的调节。精子是高度分化的特殊细胞,不具有转录活性,主要依赖于蛋白质的磷酸化完成精子成熟、分化和受精等过程。因此,对于精子磷酸化蛋白质组学的研究有助于进一步了解精子发生、精子获能、超激活以及精卵识别等过程的调控机制。本文简要综述了精子磷酸化蛋白质组学的研究方法及磷酸化蛋白质组学在精子中的应用,为精子磷酸化蛋白质组学在实际科研应用中提供了理论参考。
Abstract:Protein phosphorylation is one of the most important and extensive post-translation modification in organisms, which is involved in the regulation of various processes. Sperm is a highly differentiated cell, without transcriptional activity, and sperm maturation, differentiation and fertilization rely on protein phosphorylation.Therefore, the study of sperm phosphorylation proteomics will helpful to illustrate the regulatory mechanism of spermatogenesis, sperm capacitation, hyperactivation and sperm-oocyte recognition. Here, we briefly summary the strategy of phosphorylation-enrichment and the application of sperm phosphorylation proteomics, which would provide a theoretical reference for the application of sperm phosphorylation proteomics in practical research.
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基本信息:
DOI:10.13417/j.gab.037.001842
中图分类号:Q492
引用信息:
[1]候振,付强,黄愉淋,等.精子磷酸化蛋白质组学研究应用[J].基因组学与应用生物学,2018,37(04):1842-1846.DOI:10.13417/j.gab.037.001842.
基金信息:
广西自然科学基金(2016GXNSFAA380225);; 广西水牛遗传繁育重点实验室开放课题(SNKF-2015-02)共同资助
2018-04-25
2018-04-25