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本试验研究了色氨酸合成酶合成2-L-甲基色氨酸,验证色氨酸合成酶基因工程菌活性,用单因素实验及响应面分析法对色氨酸合成酶合成2-L-甲基色氨酸的合成条件进行优化。结果表明,最佳的合成条件:pH=8.06,温度为33.3℃,L-丝氨酸浓度为12.7 g/L,此条件下,手性药物2-L-甲基色氨酸的合成量为10.5 g/L。研究结果可为手性药物2-L-甲基色氨酸工业化生产提供参考价值。
Abstract：Synthesis of 2-L-methyl t ryptophan by tryptophan synthase was studied and activity of tryptophan synthetase gene engineering bacteria was verified in this experiment. The synthesis reaction conditions of2-L-methyl tryptophan by tryptophan synthase were optimized with single-factor experiment and response surface methodology. The results showed that the optimal synthesis conditions were that: pH was 8.06, temperature was 33.3℃, concentration of L-serine was 12.7 g/L. Under this condition, synthesis quantity of chiral drug2-L-methyltryptophan was 10.5 g/L. This research should provide a reference for industrial production of chiral drug 2-L-methyltryptophan.
Chen Y.L.,Wei X.Q.,Du L.Q.,Wei Y.T.,and Huang R.B.,2014,Irrational design directed evolution of glycerol dehydratase of Klebsiella pneumoniae in vitro,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(1):42-50(陈云来,韦旭钦,杜丽琴,韦宇拓,黄日波,2014,基于非理性设计的肺炎克雷伯氏杆菌甘油脱水酶的分子定向进化,基因组学与应用生物学,33(1):42-50)
Cheng L.K.,Xu Q.Y.,Xie X.X.,and Chen N.,2010,Quick determination of L-tryptophan in fermented broth by HPLC,Tianjin Keji Daxue Xuebao(Journal of Tianjin University of Science&Technology),25(1):9-12(程立坤,徐庆阳,谢希贤,陈宁,2010,HPLC快速测定发酵液中L-色氨酸含量,天津科技大学学报,25(1):9-12)
Huang Y.J.,Liu A.F.,and Yao L.,1987,Synthesis of DL-5-methyltryptophan,Anjisuan Zazhi(Journal of Amino Acid),4:1-3(黄宜基,刘爱福,姚莉,1987,DL-5-甲基色氨酸的化学合成,氨基酸杂志,4:1-3)
Jakoblinnert A.,and Rother D.,2014,A two-step biocatalytic cascade in micro-aqueous medium:using whole cells to obtain high concentrations of a vicinal diol,Green Chem.,16:3472-3482
Karel H.,Igor Z.,Giovanna P.,Isabel U.,Claudia M.W.,Jordi B.,Joglar J.,Teodor P.,and Pere C.,2015,Engineered L-serine hydroxymethyltransferase from Streptococcus thermophilus for the synthesis ofa,a-dialkyl-a-amino acids,Angew.Chem.Int.Ed.,54:3013-3017
Li W.Q.,Mu W.M.,Jiang B.,Zhang T.,and Miao M.,2011,Optimization of enzymatic synthesis of glutamylmethylamide with response surface methodology,Shiping Gongye Keji(Science and Technology of Food Industry),32(1):215-218(李伟桥,沐万孟,江波,张涛,缪铭,2011,响应面优化-谷氨酰甲胺酶法合成条件的研究,食品工业科技,32(1):215-218)
Mark D C.,and Arthur J.R.,2015,Laccase-catalyzed synthesis of2,3-ethylenedithio-1,4-quinones,Journal of Molecular Catalysis B:Enzymatic,119:85-89
Willem P.D.,Claudia B.,Marco W.F.,and Andrea M.,2015,Structure-based enzyme tailoring of 5-hydroxymethylfurfural oxidase,ACS Catal.,5:1833-1839
Willem P.D.,Daphne E.G.,Marco W.F.,and Andrea M.,2014,Enzyme-catalyzed oxidation of 5-hydroxymethylfurfural to Furan-2,5-dicarboxylic acid,Angew.Chem.Int.Ed.,53:6515-6518
Xu F.,Deng Z.X.,and Lin S.J.,2013,Tryptophan,an important starting material in biosynthesis of microbial natural products,Weishengwu Tongbao(Microbiology China),40(10):1796-1809(徐飞,邓子新,林双君,2013,色氨酸,天然产物生物合成中的重要结构单元,微生物学通报,40(10):1796-1809)
Xu L.S.,Liu J.Z.,Wang Z.Y.,Zhang H.J.,Liu W.,Liu Q.,and Jiao Q.C.,2011,Enzymatic synthesis of L-tryptophan catalyzed by tryptophan synthase in a water/organic solvent biphase system,Cuihua Xuebao(Chinese Journal of Catalysis),32(8):1405-1410(徐礼生,刘均忠,王治元,张宏娟,刘伟,刘茜,焦庆才,2011,水/有机溶剂双相体系中色氨酸合成酶酶法L-色氨酸,催化学报,32(8):1405-1410)
Zhou G.X.,Chen G.Y.,and Yan B.B.,2015,Two-step biocatalytic process using lipase and whole cell catalysts for biodiesel production from unrefined jatropha oil,Biotechnol.Lett.,37:1959-1963
基本信息:
DOI：10.13417/j.gab.035.001143
中图分类号:TQ464.7
引用信息:
[1]徐礼生,高贵珍,赵亮,等.响应面法优化色氨酸合成酶合成2-L-甲基色氨酸[J].基因组学与应用生物学,2016,35(05):1143-1148.DOI:10.13417/j.gab.035.001143.
基金信息:
安徽省优秀青年人才基金重点项目(2013SQRL086ZD);; 宿州学院教授(博士)科研启动基金项目(2014jb06);宿州学院质量工程项目(szxyzyjh201402);宿州学院校级平台项目(2014YKF17)共同资助;; 宿州区域发展协同创新中心开放课题(2015SZXTZXKFYB01)
2016-05-25
2016-05-25