| 110 | 3 | 41 |
| 下载次数 | 被引频次 | 阅读次数 |
为了探讨局灶性脑缺血再灌注大鼠采用Ephrin-B2干预促进大鼠神经功能的恢复及作用机制,本研究选取了72只成年雄性SD大鼠,采用随机数字表法分为:空白组(等量生理盐水)、假手术组(等量生理盐水)、模型组(等量生理盐水)和干预组(Ephrin-B2连续干预3 d),每组各18只。通过对比各组大鼠神经功能评分,检测对比各组大鼠缺血侧大脑海马组织中血管细胞间黏附分子-1(VCAM-1)、核转录因子(NF-κB)蛋白、微血管密度的水平,检测并比较各组大鼠缺血侧海马组织中血管内皮生长因子(VEGF)m RNA及蛋白的表达水平,我们发现造模后第4、8、12、14天,干预组大鼠的神经功能评分显著的低于模型组(p<0.05);造模后第4、8、12、14天,干预组、模型组大鼠的缺血侧大脑海马组织中VCAM-1、NF-κB蛋白显著的高于空白组和假手术组(p<0.05);干预组大鼠的缺血侧大脑海马组织中VCAM-1、NF-κB蛋白显著的低于模型组(p<0.05);造模后第4、8、12、14天,干预组大鼠的缺血脑组织中微血管密度显著的高于模型组(p<0.05);造模后第4、8、12、14天,干预组、模型组大鼠的血侧脑组织VEGF m RNA及蛋白显著的高于空白组和假手术组(p<0.05);干预组大鼠的血侧脑组织VEGF m RNA及蛋白显著的高于模型组(p<0.05)。本研究表明,局灶性脑缺血再灌注大鼠采用Ephrin-B2干预能促进大鼠神经功能的恢复,可能与促进新生血管形成有关。
Abstract：In order to investigate the effect of Ephrin-B2 intervention on the recovery of neurological function in rats with focal cerebral ischemia and reperfusion, we selected 72 adult male SD rats were randomly divided into control group(saline), sham operation group(saline), model group(saline) and intervention group(intervention Ephrin-B2 for continuous 3 days), with 18 rats in each group. With the comparison of neural function score in rats,vascular cell adhesion molecule-1(VCAM-1), a nuclear transcription factor(NF-κB) protein level, and microvessel density were detected and compared in hippocampus of brain tissue bleeding side, vascular endothelial growth factor(VEGF) m RNA and protein expression level were detected in the hippocampus tissue of ischemia. We found that four, eight, twelve, and fourteen days after modeling, neural function score in the intervention group was significantly lower than that in the model group(p<0.05). Four, eight, twelve, fourteen days after modeling, VCAM-1,NF-κB protein in hippoca mp us of brain hemorrh age side in the model group and intervention group weresignificantly higher than those of blank group and sham operation group(p<0.05). VCAM-1, NF-κB protein of brain hemorrhage in the intervention group were significantly lower than those in the model group(p<0.05). Four, eight,twelve, 14 days after modeling, microvessel density of ICH in the intervention group was significantly higher than that in model group(p<0.05). Four, eight, twelve, 14 days after modeling blood, VEGF m RNA and protein in intervention group and model group were significantly higher than those in the control group and sham operation group(p<0.05). In addition, VEGF m RNA and protein of blood brain tissue were significantly higher than in the intervention group compared with those in model group(p<0.05). Our research indicated that the intervention of Ephrin-B2 could promote the recovery of neurological function in rats with focal cerebral ischemia and reperfusion, which might be associated with the form of neovascularization.
Bonaldo M.F.,Yu M.T.,Jelenc P.,Brown S.,Su L.,Lawton L.,Deaven L.,Efstratiadis A.,Warburton D.,and Soares M.B.,1994,Selection of c DNAs using chromosome-specific genomic clones:application to human chromosome 13,Hum.Mol.Genet.,3(9):1663-1673
Camandola S.,Poli G.,and Mattson M.P.,2000,The lipid peroxidation product 4-hydroxy-2,3-nonenal inhibits constitutive and inducible activity of nuclear factor kappa B in neurons,Brain.Res.Mol.Brain Res.,85(1-2):53-60
Chen Y.M.,Chen T.P.,and Feng H.L.,2012,Mechanism and therapy state of cerebral ischemia reperfusion injury,Yixue Yanjiu Yu Jiaoyu(Medical Research and Education),29(6):47-54(陈玉敏,陈涛平,冯浩楼,2012,脑缺血再灌注损伤机制与治疗现状,医学研究与教育,29(6):47-54)
Clemens J.A.,2000,Cerebral ischemia:gene activation,neuronal injury,and the protective role of antioxidants,Free Radic.Biol.Med.,28(10):1526-1531
Dai B.,Wang W.,Ma Y.,Liu R.,and Zhang Y.,2016,A taspine derivative supresses Caco-2 cell growth by competitively targeting Ephrin-B2 and regulating its pathway,Oncol.Rep.,36(3):1526-1534
Han J.T.,Dong X.Q.,and Zhang W.C.,2005,Progress in the studies of Eph B2 receptor protein tyrosine kinase and its lig ands,Wujing Houqin Xueyuan Xuebao(Yixue Ban)(Journal of Logistics University of PAP(Medical Sciences)),14(2):136-138(韩景田,董小青,张文成,2005,酪氨酸蛋白激酶Eph B_2受体及其配体研究进展,武警后勤学院学报(医学版),14(2):136-138)
Liu X.,Luo Q.,Zheng Y.,Liu X.,Luo Q.,Zheng Y.,Liu X.,Hu Y.,Liu W.,Luo M.,Zhao Y.,and Zou L.,2016,Notch4 sig naling controls ephrin B2-induced endothelial progenitor cell dysfunction in preeclampsia,Reproduction,152(1):47-55
Liu Y.S.,Han L.,and Qin W.H.,2016,Study on the relationship between carotid stenosis and ischemic stroke,Zhongguo Shequ Yishi(Chinese Community Doctors),32(2):25-26(刘彦书,韩丽,秦文花,2016,对于颈动脉狭窄与缺血性脑卒中相关性的研究,中国社区医师,32(2):25-26)
Min W.U.,Wenting A.I.,Chen L.,Zhao S.,and Liu E.,2016,Bradykinin receptors and Eph B2/Ephrin B2 pathway in response to high glucose-induced osteoblast dysfunction and hyperglycemia-induced bone deterioration in mice,Int.J.Mol.Med.,37(3):565-574
Planagum觓J.,Haselmann H.,Mannara F.,Planagum觓J.,Haselmann H.,Mannara F.,Petit-Pedrol M.,Grünewald B.,Aguilar E.,R觟pke L.,Martín-García E.,Titulaer M.J.,Jercog P.,Graus F.,Maldonado R.,Geis C.,and Dalmau J.,2016,Ephrin-B2 prev ents N-methyl-D-aspartate receptor antibody effects on memory and neuroplasticity,Ann.Neurol.,80(3):388-400
Tang J.,Zhang Y.H.,Wang H.,Zhang H.L.,and Liu G.Y.,2012,Etiology classification and risk factors analysis of young pa tients with acute ischemic stroke,Linchuang Shenjingbingxue Zazhi(Journal of Clinical Neurology),25(6):465-466(唐娟,张峪涵,王宏,张慧丽,刘桂云,2012,青年缺血性脑卒中的病因学分型及危险因素分析,临床神经病学杂志,25(6):465-466)
Valverde-Franco G.,Lussier B.,Hum D.,Wu J.,Hamadjida A.,Dancause N.,Fahmi H.,Kapoor M.,Pelletier J.P.,and Mar tel-Pelletier J.,2016,Cartilage-specific deletion of ephrin-B2in mice results in early developmental defects and an osteoarthritis-like phenotype during aging in vivo,Arthritis Res.Ther.,18(1):65
Wang A.Y.,2017,The Expression and clinical significance of thr ombomodulin and vascular cell adhesion molecule-1 in patients with ischemic stroke,Guiyang Yixueyuan Xuebao(Journal of Guiyang Medical College),42(8):960-963(王爱岳,2017,血栓调节蛋白和血管细胞黏附分子-1在缺血性脑卒中患者外周血中的表达,贵阳医学院学报,42(8):960-963)
Wang L.P.,and Cheng Z.F.,2017,Vascular cell adhesion molecule1 and its correlation with gout and other metabolic diseases,Yixue Zongshu(Medical Recapitulate),23(11):2111-2115(王丽培,成志锋,2017,血管细胞黏附分子1及其与痛风和其他代谢性疾病的相关性,医学综述,23(11):2111-2115)
Wang Y.,Nakayama M.,Pitulescu M.E.,Schmidt T.S.,Bochenek M.L.,Sakakibara A.,Adams S.,Davy A.,Deutsch U.,Lüthi U.,Barberis A.,Benjamin L.E.,M覿kinen T.,Nobes C.D.,and Adams R.H.,2010,Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis,Nature,465(7297):483-486
Xiao H.,Gu W.P.,Hu Y.,and Wang Z.,2014,Ephrin-B2 enhances expression of VEGF and angiogenesis after focal cerebral ischemia in rats,Xiandai Shengwu Yixue Jinzhan(Progress in Modern Biomedicine),14(30):5824-5828(肖慧,谷文萍,胡珏,王振,2014a,Ephrin-B2促进大鼠局灶脑缺血再灌注后VEGF表达及血管新生,现代生物医学进展,14(30):5824-5828)
Xiao H.,Gu W.P.,Yang Q.D.,and Zeng X.,2014,Expression of Ephrin-B2 after focal cerebral ischemia in rats,Zhongnan Daxue Xuebao(Yixue Ban)(Journal of Central South University(Medical Science)),39(5):452-457(肖慧,谷文萍,杨期东,曾学辉,2014b,大鼠局灶脑缺血再灌注后Ephrin-B2的表达,中南大学学报(医学版),39(5):452-457)
Zhao X.Q.,Deng C.Y.,Li S.Y.,Zhang J.X.,He Y.G.,Yu H.,and Liu B.,2016,Neuroprotective effect of dl-3n-butylphthalide on focal cerebral ischemia reperfusion injury in rats,Shandong Daxue Xuebao(Yixueban)(Journal of Shandong University(Health Sciences)),54(4):25-31(赵秀芹,邓春颖,李世英,张晋霞,贺永贵,余红,刘斌,2016,丁苯酞注射液对大鼠局灶性脑缺血再灌注损伤的神经保护作用,山东大学学报(医学版),54(4):25-31)
Zhong J.,Qu Y.M.,Han S.,Luo Y.L.,and Li J.F.,2017,VCAM-1improves migration and invasion of human glioma cell lines,Jichu Yixue Yu Linchuang(Basic&Clinical Medicine),37(1):19-24(钟杰,曲彦明,韩松,罗艳琳,李俊发,2017,血管细胞黏附分子-1增强人脑胶质瘤细胞系迁移和侵袭能力,基础医学与临床,37(1):19-24)
基本信息:
DOI：10.13417/j.gab.036.004906
中图分类号:R-332;R743.3
引用信息:
[1]李静,王继光,魏琰,等.Ephrin-B2对脑缺血大鼠模型神经功能恢复的促进作用及机制研究[J].基因组学与应用生物学,2017,36(12):4906-4913.DOI:10.13417/j.gab.036.004906.
基金信息:
哈励逊国际和平医院资助
2017-12-25
2017-12-25