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三羧酸转运体系(柠檬酸,苹果酸和丙酮酸)可为脂肪酸生物合成提供原料乙酰辅酶A与还原力NADPH;而苹果酸酶可催化苹果酸发生氧化脱羧而产生NADPH,是调控脂肪酸代谢的重要酶。山杏种子脂肪酸含量丰富、产油率高,是研究植物油脂代谢的良好材料。本研究以山杏为试材,采用RACE克隆和电子拼接相结合的方法,首次从山杏种子中克隆得到苹果酸酶基因(命名为SaME,GenBank上登记号为JX262381),该基因的cDNA编码区全长1923bp、编码640个氨基酸,预测其编码蛋白分子量为70.15kD、等电点为6.38;同时,运用生物信息学方法对SaME基因编码蛋白的理化特性、结构域和亚细胞定位等方面进行预测分析,结果显示,该蛋白定位在细胞的质体膜上,具有苹果酸酶活性,属于NADP-ME超家族。本文对山杏SaME基因的克隆与分析,为进一步探究苹果酸酶对油脂代谢的调控机制奠定了基础。
Abstract：The tricarboxylic acid transport system is composed of citrate, malate and pyruvate, and its main physiological function is to provide the raw materials acetyl-CoA and the reducing power NADPH necessary for the biosynthesis of fatty acids. Malic enzyme, by which malate is converted to pyruvate via the tricarboxylic acid transport system with the production of NADPH, is the main regulated enzyme of the fatty acid biosynthesis in the plants. Prunus armeniaca is a kind of oil woody plant with high oil content and present a high rate of oil production in the seeds. It is an ideal material to study the mechanism of plant lipid metabolism. In this paper, a gene encoding malic enzyme, named as SaME (JX262381), was cloned from P. armeniaca by RACE and electronic splicing tech niques. This full-length cDNA contained an open reading frame of 1 923 bp in length, encoding a polypeptide of 640 amino acid residues with a molecular mass of 70.15 kD and a pI of 6.38. And because of the bioinformatics analysis, the ME protein, localized in the plasma membrane, has the activity of malic enzyme, and it belongs to the superfamily of NADP-ME. The analysis of SaME gene in P. armeniaca provides a theoretical basis to further explore of malic enzyme regulation in lipid metabolism.
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基本信息:
中图分类号:S662.2
引用信息:
[1]章文乐,蔡兼,钮俊,等.山杏苹果酸酶基因的克隆与生物信息学分析[J].基因组学与应用生物学,2013,32(03):332-338.
基金信息:
国家自然科学基金项目(J1103516);; “十二五”农村领域国家科技计划课题(2011BAD22B08)共同资助
2013-06-28
2013-06-28