| 337 | 21 | 43 |
| 下载次数 | 被引频次 | 阅读次数 |
Micro RNAs(miRNAs)在植物的生长发育以及植物对环境的适应方面发挥了重要的作用,成为近几年研究的热点。mi R172家族是一个保守的mi RNA家族,之前的研究表明mi R172的作用涉及生长期过渡、闭花受精、花器官发育、开花时间调控等方面。但是到目前为止,人们对大豆mi R172家族的了解还不是很清楚。本实验通过mi RBase、PLACE数据库及DNAman软件对gma-mi R172家族成员的前体序列、成熟序列、启动子序列进行比较分析,发现gma-mi R172家族成员成熟序列虽然高度保守,但前体序列具有一定的差异,这可能导致了各成员的功能差异;启动子分析显示其启动子含有光反应原件和多种非生物胁迫响应元件,推测其可能参与大豆的开花调控和逆境胁迫响应。通过实时定量PCR分析了大豆mi R172成员的组织特异性表达情况。利用PMRD数据库对gma-mi R172的靶基因进行了生物信息学分析,获得了7个AP2-like类转录因子,参与花器官发育、开花时间调控及非生物胁迫响应的方面。上述结果初步分析了大豆mi R172家族的功能与靶基因,对今后进一步分析研究大豆mi R172家族及其靶基因打下了基础。
Abstract：Micro RNAs(mi RNAs) play crucial roles in many aspects of plant development and responses to the environment, it has become one of popular research topic recent years. The mi R172 family is a conserved mi RNA,they have been extensively studied in growth-phase transition, cleistogamy, flower development and flowering-time control in previous studies. But so far, the function of gma-mi R172 is not clear to us. In the present study, the premi RNAs sequence, mature sequence and promoter of gma-mi R172 were compared and analyzed through mi RBase,PLACE databases and DNAman. Analysis results showed that the sequence similarity between mature mi R172 family members is very high, but it varies among the pre-mi RNAs, this may caused the functional differences between the members. Promoter analysis showed that many important photoreaction and abiotic stress cis-acting regulatory elements were found in the promoter sequence, speculating it may participate in soybean flowering regulation and adversity stress response. We analyzed the expression profile of gma-mi R172 in different tissues. Seven AP2-like transcription factor target genes of gma-mi R172 were identified by PMRD software, which involved in flower development and flowering-time control and abiotic stress responses. These results preliminary reveals the function of gma-mi R172, laying foundation for the future analytical investigation of gma-mi R172 and their target genes.
Aukerman M.J.,and Sakai H.,2003,Regulation of flowering time and floral organ identity by a micro RNA and its APETALA2-like target genes,Plant Cell,15(11):2730-2741
Bartel D.P.,2004,Micro RNAs:genomics,biogenesis,mechanism,and function,Cell,116(2):281-297
Gupta O.P.,Meena N.L.,Sharma I.,and Sharma P.,2014,Differential regulation of micro RNAs in response to osmotic,salt and cold stresses in wheat,Molecular Biology Reports,41(7):4623-4629
Higo K.,Ugawa Y.,Iwamoto M.,and Korenaga T.,1999,Plant cisacting regulatory DNA elements(PLACE)database,Nucleic Acids Research,27:297-300
Hu H.W.,Hou X.Y.,Cai J.,Niu J.,Xiang Z.,An J.Y.,and Lin S.Z.,2014,Prediction and expressing analysis of the cold stress-responsive mi R475 target genes from Populus suaveolens,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(3):487-494(胡惠雯,侯欣瑜,蔡兼,钮俊,项争,安基永,林善枝,2014,低温胁迫响应的甜杨mi R475靶基因的预测及表达,基因组学与应用生物学,33(3):487-494)
Joshi T.,Yan Z.,Libault M.,Jeong D.H.,Park S.,Green P.J.,Sherrier D.J.,Farmer A.,May G.,Meyers B.C.,Xu D.,and Stacey G.,2010,Prediction of novel mi RNAs and associated target genes in Glycine max,BMC Bioinformatics,11(S1):S14
Lauter N.,Kampani A.,Carlson S.,Goebel M.,and Moose S.P.,2005,micro RNA172 down-regulates glossy15 to promote vegetative phase change in maize,Proc.Natl.Acad.Sci.USA,102(26),9412-9417
Li H.Y.,Dong Y.Y.,Yin H.L.,Wang N.,Yang J.,Liu X.M.,Wang Y.F.,Wu J.Y.,and Li X.K.,2011,Characterization of the stress associated micro RNAs in Glycine max by deep sequencing,BMC Plant Biol.,11:170
Park W.,Li J.J.,Song R.T.,Messing J.,and Chen X.M.,2002,CARPEL FACTORY,a dicer homolog,and HEN1,a novel protein,act in micro RNA metabolism in Arabidopsis thaliana,Current Biology,12(17):1484-1495
Subramanian S.,Fu Y.,Sunkar R.,Barbazuk W.B.,Zhu J.K.,and Yu O.,2008,Novel and nodulation-regulated micro RNAs in soybean roots,BMC Genomics,9:160
Voinnet O.,2009,Origin,biogenesis,and activity of plant micro RNAs,Cell,136(4):669-687
Zhang B.H.,Pan X.P.,and Stellwag E.J.,2008,Identification of soybean micro RNAs and their targets,Planta,229(1):161-182
Zhou L.G.,Liu Y.H.,Liu Z.C.,Kong D.Y.,Duan M.,and Luo L.J.,2010,Genome-wide identification and analysis of droughtresponsive micro RNAs in Oryza sativa,Journal of Experimental Botany,61(15):4157-4168
基本信息:
DOI：10.13417/j.gab.034.001277
中图分类号:S565.1
引用信息:
[1]王涛,李永光,李文滨.大豆miR172家族成员序列及表达模式初步分析[J].基因组学与应用生物学,2015,34(06):1277-1282.DOI:10.13417/j.gab.034.001277.
基金信息:
国家核心大豆基因工程项目(2014ZX08004002);; 国家自然科学基金(31201228)共同资助
2015-06-25
2015-06-25