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水稻细菌性条斑病菌(Xamthomonas.oryzae pv.oryzicola,Xoc)是水稻的主要病原菌之一,其引发的水稻细菌性条斑病可造成水稻严重减产。本文利用Xoc GX01作为实验菌株,通过细菌培养、蛋白提取、纯化获得总蛋白,利用高p H值C18反相色谱作为第一维分离方式,再将收集的馏分通过LC-MS/MS技术得到了总蛋白质表达谱,共鉴定蛋白质1 621个,同时利用生物信息学技术对鉴定蛋白进行基因本体(gene ontology,GO)生物学分类,对生物功能、生物进程以及细胞组件进行了注释。为明确植物病原菌的作用机理和研究植物-病原微生物互作提供了方法学基础。
Abstract：Xamthomonas. oryzae pv. oryzicola(Xoc), the causal agent of rice bacterial leaf streak, is one of the most serious diseases of rice. It can cause a severe reduction of rice output. In this study, Xoc GX01 isolated was used as experimental strain, we got the total proteins by bacterial culture, extraction and purification of protein. Firstly,used high p H C18 reverse phase chromatography as the first dimensional separation, then got the intracellular protein expression spectrum using LC-MS/MS, finally identificated 1 621 proteins. Meanwhile biological function,biological process and cellular component had been carried on the Gene Ontology(GO) annotation. This result provides the basis for the further study of the mechanism of the plant pathogenic bacteria and plant-pathogenic microorganism interactions of Xanthomonas oryzae pv. oryzicola.
Alfano J.R.,and Collmer A.,1996,Bacterial pathogens in plants:life up against the wall,Plant Cell,8(10):1683-1698
Andrade A.E.,Silva L.P.,Pereira J.L.,Noronha E.F.,Reis F.B.Jr.,Bloch C.Jr.,dos Santos M.F.,Domont G.B.,Franco O.L.,and Mehta A.,2008,In vivo proteome analysis of Xanthomonas campe stris pv.campestris in the interaction with the host plant Brassica oleracea,FEMS Microbiol.Let.,281(2):167-174
Cen W.J.,Yu K.,Gan Y.L.,Huang D.L.,Fu Q.,and Jiang B.L.,2014,Expression profile analysis of secreted proteins in Xanthomonas campestris pv.campestris,Jiyinzuxue Yu Yingyong Shengwuxe(Genomics and Applied Biology),33(2):386-391(岑卫健,于凯,甘永亮,黄德伦,付强,姜伯乐,十字花科黑腐病菌分泌蛋白表达谱分析,基因组学与应用生物学,33(2):386-391)
Chen F.Y.,Huang Q.Y.,Zhang H.X.,Lin T.,Guo Y.C.,Lin W.X.,and Chen L.,2007,Proteomic analysis of rice cultivar Jiafuzhan in the responses to Xanthomonas campestris pv.oryzicola infection,Zuowu Xuebao(Acta Agronomica Sinica),33 (7):1051-1058(陈芳育,黄青云,张红心,林涛,郭玉春,林文雄,陈亮,2007,水稻品种“佳辐占”应答细菌性条斑病病原菌侵染的蛋白质组学分析,作物学报,33(7):1051-1058)
Kocevar N.,Grazio S.F.,and Komel R.,2014,Two-dimensional gel electrophoresis of gastric tissue in an alkaline p H range,Proteomics,14(2-3):311-321
Link A.J.,Eng J.,Schieltz D.M.,Carmack E.,Mize G.J.,Morris D.R.,Garvik B.M.,and Yates J.R.,1999,Direct analysis of protein complexes using Masspectrometry,Nat.Biotechnol.,17:676-682
Mehta A.,and Rosato Y.B.,2001,Differentially expressedproteins in the interaction of Xanthomonas axonopodis pv.citri with leaf extract of the host plant,Proteomics,1(9):1111-1118
Qian G.L.,Zhou Y.J.,Zhao Y.C.,Song Z.W.,Wang S.Y.,Fan J.Q.,Hu B.S.,Venturi V.,and Liu F.Q.,2013,Proteomic analysis reveals novel extracellular virulence-associated proteins and functions regulated by the diffusible signal factor(DSF)in Xanthomonas oryzae pv.oryzicola,J.Proteome Res.,12(7):3327-3341
Schirmer E.C.,Florens L.,Guan T.L.,Yates J.R.,and Gerace L.,2003,Nuclear membrane proteins with potential disease links found by subtractive proteomics,Science,301(5638):1380-1382
Stephanowitz H.,Lange S.,Lang D.,Freund C.,and Krause E.,2012,Improved two-simensional reversed phase-reversed phase LC-MS/MS approach for identification of peptide-protein interactions,J.Proteome Res.,11(2):1175-1183
Swings J.,van den Mooter M.,Vauterin L.,Hoste B.,Gillis M.,Mew T.W.,and Kersters K.,1990,Reclassification of the causal agents of bacterial blight(Xanthomonas campestris pv.oryzae)and bacterial leaf streak(Xanthomonas campestris pv.oryzicola)of rice as pathovars of Xanthomonas oryzae(exIshiyama 1922)sp.nov.,nom.rev,Int.J.Syst.Bacteriol.,40:309-311
Xiao Y.S.,Wei X.X.,Gao H.P.,Niu X.N.,Cen Z.L.,Huang P.,Wu Y.,Wang R.,and He Y.Q.,2011,Characterization of a Xanthomonas oryzae pv.oryzicola strain and the establishment of its genetic manipulation system,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),30:1211-1217(肖永胜,韦雪雪,郜惠苹,牛祥娜,岑贞陆,黄萍,吴玥,汪嵘,何勇强,2011,一株水稻细菌性条斑病菌的鉴定与遗传操作系统的建立,基因组学与应用生物学,30:1211-1217)
Zhao Y.C.,Qian G.L.,Yin F.Q.,Fan J.Q.,Zhai Z.W.,Liu C.H.,Hu B.S.,and Liu F.Q.,2011,Proteomic analysis of the regulatory function of DSF-dependent quorum sensing in Xanthomonas oryzae pv.oryzicola,Microbial Pathogenesis,50(1):48-55
Zou H.S.,Yuan L.,Guo W.,Li Y.R.,Che Y.Z.,Zou L.F.,and Chen G.Y.,2011,Construction of a Tn5-tagged mutant library of Xanthomonas oryzae pv.oryzicola as an invaluable resource for functional genomics,Curr.Microbiol.,62(3):908-916
基本信息:
DOI：10.13417/j.gab.034.001239
中图分类号:S435.111.4
引用信息:
[1]朱平川,覃宏平,付珊,等.基于LC-MS-MS技术建立水稻细菌性条斑病菌的蛋白质表达谱的研究[J].基因组学与应用生物学,2015,34(06):1239-1244.DOI:10.13417/j.gab.034.001239.
基金信息:
广西自然科学研究基金项目(20130231);; 广西教育厅基金重点项目(20130300);; 广西教育厅高校科学技术研究一般项目(KY2015YB012);; 广西研究生教育创新计划资助项目(YCBZ2015006)共同资助
2015-06-25
2015-06-25