| 225 | 13 | 22 |
| 下载次数 | 被引频次 | 阅读次数 |
4-羟基-3-甲基-2-(E)-丁烯基-4-磷酸还原酶(4-hydroxy-3-methyl-2-(E)-butenyl-4-diphosphate reductase,HDR)是异戊烯基焦磷酸合成途径之一甲基赤藓糖磷酸(methylerythritol phosphate,MEP)途径中的最后一个酶,催化4-羟基-3-甲基-(2E)-丁烯基-4-磷酸生成异戊烯基焦磷酸。根据植物HDR的同源序列设计引物,通过RT-PCR结合RACE的方法在橡胶树中获得了与其相应的HDR基因,命名为HbHDR。序列分析表明HbHDR长1627bp,编码462个氨基酸,属于LYTB家族,该氨基酸序列与烟草、长春花、胡黄连、拟南芥、银杏和火炬松的HDR同源性为79.1%、78.4%、76.5%、75.3%、72.2%和70.9%。半定量RT-PCR结果显示,乙烯诱导胶乳HbHDR的表达。
Abstract：4-hydroxy-3-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) is proved to be the terminal-acting enzyme in the plastid MEP pathway which provides isoprenoids. The full-length cDNA encoding a HDR, designated as HbHDR, was isolated for the first time from Hevea brasiliensis by the RACE method. HbHDR contained a 1 627-bp open reading frame encoding 462 amino acids. The deduced HbHDR, which shared 79.1%, 78.4%, 76.5%, 75.3%, 72.2% and 70.9% identities with the HDR protein from Nicotiana langsdorffii×Nicotiana sandera, Catharanthus roseus, Picrorhiza kurrooa, Arabidopsis thaliana, Ginkgo biloba and Pinus taeda, belonged to the LYTB family. Transcription pattern analysis revealed that HbHDR was constitutively expressed in laticifer, barks and leaf. The transcription of HbHDR in latex was induced by ethephon.
Adam P.,Hecht S.,Eisenreich W.,Kaiser J.,Gr覿wert T.,ArigoniD.,Bacher A.,and Rohdich F.,2002,Biosynthesis of ter-penes:studies on1-hydroxy-2-methyl-2-(E)-butenyl4-diphosphate reductase,Proc.Natl.Acad.Sci.,USA.,99:12108-12113
Archer B.L.,and Audley B.G.,1987,New aspects of rubberbiosynthesis,Bot.J.Linn.Soc.,94:181-196
Botella-Pavia P.,Besumbes O.,Phillips M.A.,Carretero-Paulet L.,Boronat A.,and Rodríguez-Concepci仵n M.,2004,Regula-tion of carotenoid biosynthesis in plants:evidence for a keyrole of hydroxymethylbutenyl diphosphate reductase in con-trolling the supply of plastidial isoprenoid precursors,PlantJ.,40:188-199
Cornish K.,2001,Similarities and differences in rubber biochem-istry among plant species,Phytochemistry,57(7):1123-1134
Eisenreich W.,Rohdich F.,and Bacher A.,2001,Deoxyxylulosephosphate pathway to terpenoids,Trends Plant Sci.,6(2):78-84
Eisenreich W.,Schwarz M.,Cartayrade A.,Arigoni D.,Zenk M.H.,and Bacher A.,1998,The deoxyxylulose phosphate pathwayof terpenoid biosynthesis in plants and microorganisms,Chem.Biol.,5(9):R221-R233
Hao B.Z.,and Wu J.L.,2000,Laticifer differentiation in Heveabrasiliensis:induction by exogenous jasmonic acid andlinolenic acid,Ann.Bot.,85:37-43
Hsieh M.H.,and Goodman H.M.,2005,The Arabidopsis IspHhomolog is involved in the plastid nonmevalonate pathwayof isoprenoid biosynthesis,Plant Physiol.,138:641-653
Jr Cunningham F.X.,Lafond T.P.,and Gantt E.,2000,Evidenceof a role for LytB in the nonmevalonate pathway of iso-prenoid biosynthesis,J.Bacteriol.,182(20):5841-5848
Kim S.M.,Kuzuyama T.,Kobayashi A.,Sando T.,Chang Y.J.,and Kim S.U.,2008,1-Hydroxy-2-methyl-2-(E)-butenyl4-diphosphate reductase(IDS)is encoded by multicopygenes in gymnosperms Ginkgo biloba and Pinus taeda,Plan-ta,227(2):287-298
Ko J.H.,Chow K.S.,and Han K.H.,2003,Transcriptome analy-sis reveals novel features of the molecular events occurringin the laticifers of Hevea brasiliensis(para rubber tree),Plant Molecular Biology,53(4):479-492
Kush A.,1994,Isoprenoid biosynthesis:the Hevea factory,PlantPhysiol.Biochem.,32(6):761-767
Liu W.P.,Wang M.J.,Han Y.Z.,and Zhao D.G.,2008,Biosynthet-ic mechanism of natural rubber,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),38(4):382-388(刘卫平,王敏杰,韩玉珍,赵德刚,2008,天然橡胶的生物合成机制,植物生理学通讯,38(4):382-388)
Lu J.,Wu W.,Cao S.,Zhao H.,Zeng H.,Lin L.,Sun X.,andTang T.,2008,Molecular cloning and characterization of1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reduc-tase gene from Ginkgo biloba,Mol.Biol.Rep.,35(3):413-420
Rohdich F.,Hecht S.,G覿rtner K.,Adam P.,Krieger C.,Am-slinger S.,Arigoni D.,Bacher A.,and Eisenreich W.,2002,Studies on the nonmevalonate terpene biosynthetic pathway:metabolic role of IspH(LytB)protein,Proc.Natl.Acad.Sci.,USA,99(3):1158-1163
Sambrook J.,Fritsch E.R.,and Maniatis T.,eds.,1989,Cloningand analysis of eukaryotic genomes;in molecular cloning:alaboratory manual,Cold Spring Harbor Laboratory Press,Cold Spring Harbor,NY,pp.456-489
Sando T.,Takaoka C.,Mikai T.,Yamashita A.,Hattori M.,Oga-sawara N.,Fukusaki E.,and Kobayashi A.,2008,Cloningand characterization of mevalonate pathway genes in a natu-ral rubber producing plant,Hevea brasiliensis,Biosci.Biotcehnol.Biochem.,72(8):2049-2060
Seetang-Nun Y.,Sharkey T.D.,and Suvachittanont W.,2008a,Molecular cloning and characterization of two cDNAs en-coding1-deoxy-d-xylulose5-phosphate reductoisomerasefrom Hevea brasiliensis,J.Plant Physiol.,165(9):991-1002
Seetang-Nun Y.,Sharkey T.D.,and Suvachittanont W.,2008b,Isolation and characterization of two distinct classes of DXSgenes in Hevea brasiliensis,DNA Seq.,19:291-300
Tang C.R.,Qi J.Y.,Li H.P.,Zhang C.L.,and Wang Y.K.,2007,A convenient and efficient protocol for isolating high-qualityRNA from latex of Hevea brasiliensis(para rubber tree),J.Biochem.Biophys.Methods,70(5):749-754
Wang Q.,Pi Y.,Hou R.,Jiang K.J.,Huang Z.S.,Hsieh M.S.,SunX.F.,and Tang K.X.,2008,Molecular cloning and charac-terization of1-hydroxy-2-methyl-2-(E)-butenyl4-diphos-phate reductase(CaHDR)from Camptotheca acuminata andits functional identification in Escherichia coli,BMB Rep.,41:112-118
基本信息:
中图分类号:S794.1
引用信息:
[1]李辉亮,雷美玉,彭世清.巴西橡胶树4-羟基-3-甲基-2-(E)-丁烯基-4-磷酸还原酶基因(HbHDR)的克隆及表达分析[J].基因组学与应用生物学,2009,28(01):15-21.
基金信息:
国家自然科学基金(30560120;30760203);; 中央级公益性科研院所基本科研业务费专项资金资助项目(ITBBZD0712)资助
2009-02-28
2009-02-28