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本研究以不同浓度的白花蛇舌草注射液处理人胃癌SGC-7901细胞，探究其对细胞增殖的影响。利用台盼蓝拒染法和吉姆萨染色法分别检测不同浓度的白花蛇舌草注射液对人胃癌SGC-7901细胞存活率和细胞克隆形成率的影响；采用EdU法检测药物处理后SGC-7901的细胞增殖率；最后应用RT-qPCR和Western blot分别检测药物处理后对细胞周期相关蛋白CCNB1和CCND1在mRNA水平和蛋白水平表达的影响。实验结果表明：白花蛇舌草注射液可抑制人胃癌细胞SGC-7901的细胞活力，其细胞存活率随着药物浓度的升高而降低；不同浓度的白花蛇舌草注射液处理人胃癌SGC-7901细胞48 h后，其细胞克隆形成率和细胞增殖率显著降低；RT-qPCR和Western blot结果显示，CCNB1和CCND1基因的mRNA及蛋白表达水平随着药物浓度的升高而显著降低。综上所述，白花蛇舌草通过下调CCNB1和CCND1基因的表达抑制人胃癌SGC-7901细胞增殖。
Abstract：In this study, gastric cancer SGC-7901 cells were treated with Hedyotis diffusa injection in different concentrations in order to detect the effect on cell proliferation. Trypan blue and giemsa staining method were used to detect the effects of different concentrations of Hedyotis diffusa on SGC-7901 cell viability and cell clone formation. The cell proliferation rate of SGC-7901 after drug treatment was detected by EdU method.RT-qPCR and Western blot were also performed to determine the mRNA and protein levels of CCNB1 and CCND1. The results showed that Hedyotis difflosa injection could inhibit the cell viability of SGC-7901, and the cell viability decreased with the increase of drug concentration. Human gastric cancer cells SGC-7901 were treated with different concentrations of Hedyotis diffusura injection for 48 h, and the cell clone formation rate and cell proliferation rate were sig-nificantly decreased. RT-qPCR and Western blot analysis showed that the mRNA and protein levels of CCNB1 and CCND1 were significantly downregulated by Hedyotis diffusa injection in different concentrations. With the higher drug concentration, the expressions of CCNB1 and CCND1 showed a declining trend. In conclusion, Hedyotis diffusa suppresses the proliferation of gastric cancer SGC-7901 cells by inhibiting the expression of CCNB1 and CCND1.
Fang Y.F.,2016,Chk1-induced CCNB1 overexpression promotes cell proliferation and tumor growth in human colorectal cancer,Dissertation for Ph.D.,Zhejiang University,Supervisor:Cai X.J.,pp.6-7.(方益锋,2016,Chk1介导的CCNB1高表达在结直肠癌中的作用及机制研究,博士学位论文,浙江大学,导师:蔡秀军,pp.6-7.)
Jiao K.H.,Shao S.L.,Chen L.,Cui T.T.,Zhang W.W.,and Feng Y.,2018,Hedyotis diffusa induce apoptosis of the gastric cancer SGC-7901 cell,Jiyinzuxue yu Yingyong Shengwuxue(Genomics and Applied Biology),37(7):3060-3065.(焦凯贺,邵淑丽,陈丽,崔婷婷,张伟伟,冯元,2018,白花蛇舌草诱导胃癌SGC-7901细胞凋亡,基因组学与应用生物学,37(7):3060-3065.)
Li C.H.,Zhang X.J.,Xie M.,and Huang L.,2019,Clinical trial of paclitaxel combined with nedaplatin in the treatment of patients with advanced ovarian cancer,Zhongguo Linchuang Yaolixue Zazhi(The Chinese Journal of Clinical Pharmacology),35(18):2036-2039.(李常虹,章小娟,谢曼,黄翎,2019,紫杉醇联合奈达铂治疗晚期卵巢癌患者的临床研究,中国临床药理学杂志,35(18):2036-2039.)
Li F.,Yang P.M.,Cao G.S.,and Gao P.,2014,Research progress on determination methods of active constituents from Oldenlandia diffusa and its preparations,Zhongcaoyao(Chinese Traditional and Herbal Drugs),45(12):1803-1808.(李芳,杨培民,曹广尚,高鹏,2014,白花蛇舌草及其制剂有效成分定量测定方法的研究进展,中草药,45(12):1803-1808.)
Li H.,Lai Z.J.,Yang H.,Peng J.,Chen Y.Q.,and Lin J.M.,2019,Hedyotis diffusa Willd.inhibits VEGF-C-mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways,Oncol.Rep.,42(3):1225-1236.
Li Y.Y.,Shao X.Y.,Jin L.T.,Li Q.F.,and Chen X.M.,2019,Apoptosis of ovarian cancer cells induced by toosendanin through Fas/FasL signaling pathway,Zhongguo Zhongxiyi Jiehe Zazhi(Chinese Journal of Integrated Traditional and Western Medicine),39(9):1089-1094.(李雨颖,邵喜英,金莉婷,李群锋,陈旭明,2019,川楝素通过Fas/Fas L信号通路诱导人卵巢癌细胞凋亡,中国中西医结合杂志,39(9):1089-1094.)
Liu Y.Y.,Jiang Q.P.,Liu X.,Lin X.,Tang Z.B.,Liu C.,Zhou J.,Zhao M.Y.,Li X.,Cheng Z.,Li L.B.,Xie Y.Y.,Liu Z.,and Fang W.Y.,2019,Cinobufotalin powerfully reversed E-BV-miR-BART22-induced cisplatin resistance via stimulating MAP2K4 to antagonize non-muscle myosin heavy chain IIA/glycogen synthase 3β/β-catenin signaling pathway,EBioMedicine,48:386-404.
Ma D.H.,Lian F.,and Wang X.B.,2019,PLCG2 promotes hepatocyte proliferation in vitro via NF-κB and ERK pathway by targeting bcl2,myc and ccnd1,Artif.Cells Nanomed.Biotechnol.,47(1):3786-3792.
Salic A.,and Mitchison T.J.,2008,A chemical method for fast and sensitive detection of DNA synthesis in vivo,Proc.Natl.Acad.Sci.USA,105(7):2415-2420.
Sui Y.T.,Chi W.C.,and Jiang J.K.,2018,Influence of Qishan Fang combined with cisplatin on PI3K/AKt signal pathway of lung cancer A549 cells,Beijing Zhongyiyao Daxue Xuebao(Journal of Beijing University of Traditional Chinese Medicine),41(7):553-558.(隋雨桐,迟文成,姜家康,2018,芪杉方联合顺铂对肺癌A549细胞PI3K/AKt信号通路的影响,北京中医药大学学报,41(7):553-558.)
Sun K.X.,Zheng R.S.,Zhang S.W.,Zeng H.M.,Zou X.N.,Chen R.,Gu X.Y.,Wei W.Q.,and He J.,2019,Report of cancer incidence and mortality in different areas of China,2015,Zhongguo Zhongliu(China Cancer),28(1):1-11.(孙可欣,郑荣寿,张思维,曾红梅,邹小农,陈茹,顾秀瑛,魏文强,赫捷,2019,2015年中国分地区恶性肿瘤发病和死亡分析,中国肿瘤,28(1):1-11.)
Xu H.,Liu L.L.,Zhao H.,and Zhang G.J.,2018,Research progress on treatment of cancer with'couplet medicines',Zhonghua Zhongyiyao Zazhi(China Journal of Traditional Chinese Medicine and Pharmacy),33(10):4533-4538.(徐浩,刘磊楼,招欢,张光霁,2018,中医“药对”治疗癌症的研究进展,中华中医药杂志,33(10):4533-4538.)
Yang X.Z.,Tian M.H.,Yang Z.X.,Ma Y.F.,and Tian X.J.,2019,Study on extraction technology and antioxidant activity of flavonoids,polyphenols,polysaccharides in Hedyotis diffusa Willd.,Huaxue Yanjiu yu Yingyong(Chemical Research and Application),31(1):22-30.(杨新周,田孟华,杨子仙,马艳粉,田先娇,2019,白花蛇舌草中黄酮、多酚、多糖提取工艺及抗氧化研究,化学研究与应用,31(1):22-30.)
Yao Y.,Liu K.H.,and Hu X.Q.,2019,Effects of pterostilbene on lipopolysaccharide-induced inflammatory cytokine expres sion in macrophag,Shanghai Haiyang Daxue Xuebao(Journal of Shanghai Ocean University),28(1):29-36.(姚昀,刘克海,胡晓倩,2019,紫檀茋影响脂多糖诱导巨噬细胞炎症因子的变化,上海海洋大学学报,28(1):29-36.)
Yu L.,Wang F.,Guo Q.,Liu K.K.,Zhang D.Z.,and Liang D.L.,2017,Research progress on chemical constituents of Hedyotis diffusa Willd.and its pharmacological activities,Shenyang Yaoke Daxue Xuebao(Journal of Shenyang Pharmaceutical University),34(12):1104-1114.(于亮,王芳,郭琪,刘葵葵,张岱州,梁大连,2017,白花蛇舌草的化学成分及其药理活性研究进展,沈阳药科大学学报,34(12):1104-1114.)
Zhang S.P.,Guo N.,Wan G.Y.,Zhang T.,Li C.Y.,Wang Y.F.,Wang Y.S.,and Liu Y.Y.,2019,pH and redox dual-responsive nanoparticles based on disulfde-containing poly(β-amino ester) for combining chemotherapy and COX-2inhibitor to overcome drug resistance in breast cancer,J.Nanobiotechnol.,17(1):109.
基本信息:
DOI：10.13417/j.gab.040.003302
中图分类号:R285
引用信息:
[1]孙岚,邵淑丽,代飞,等.白花蛇舌草对人胃癌SGC-7901细胞增殖的影响[J].基因组学与应用生物学,2021,40(Z3):3302-3308.DOI:10.13417/j.gab.040.003302.
基金信息:
黑龙江省教育厅基本业务专项重点项目(135109104); 黑龙江省高教强省优势特色学科—玉米“粮头食尾”重点专项(LTSW201737); 黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项)(YSTSXK201809)共同资助
2020-01-08
2020-01-08
2020-01-08