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塑料凭借其优越的特性,在人类生活中被广泛应用,其中聚对苯二甲酸乙二醇酯(polyethylene terephthalate, PET)的应用范围极为广泛。PET的不当处理严重影响生态系统,干扰动植物生存,因此,PET的降解与处理已成为亟待解决的紧迫议题。生物降解法因其绿色环保的特性,成为处理PET的有效方法,其中酶解法作用高效、条件温和,符合清洁理念,是目前的研究热点。本文主要综述了酯酶、脂肪酶、角质酶和PET水解酶等降解PET常用酶的相关研究,并基于不同PET降解酶结构和功能的差异,重点阐述降解机制及限制因素以及提高PET降解效率的研究情况,为未来开发高效的降解酶技术提供新思路。
Abstract:Plastic is widely used in human life due to its unique superior characteristics, among which polyethylene terephthalate(PET) has a wide range of applications. The improper handling of PET seriously affects the ecosystem and interferes with the survival of animals and plants. Therefore, the degradation and treatment of PET have become an urgent issue that needs to be addressed. Biodegradation has become an effective method for treating PET due to its green environmental protection, and the enzymatic hydrolysis method has high efficiency, operates under mild conditions, and conforms to the concept of cleanliness, making it a current research hotspot. This paper mainly summarizes the relevant research on commonly used enzymes for PET degradation, such as esterase, lipase, cutinase and PET hydrolase. Based on the differences in the structure and function of different PET degrading enzymes, we focus on the degradation mechanism and limiting factors, improve the degradation efficiency of PET, and provide new ideas for the development of efficient degradation enzyme technologies in the future.
金昶序,2023.交叉共混预处理策略提高PET塑料的酶解效率及产物无害处理,硕士学位论文.无锡:江南大学.(JINC X,2023.Cross-blending pretreatment strategy to improve the PET enzymatic degradation efficiency and pro-duct detoxication,Thesis for M.S.Wuxi:Jiangnan University.)
康菡子,袁璐璇,王彦博,等,2021.废旧PET聚酯回收利用进展.塑料,50 (5):61-66.[KANG H Z,YUAN L X,WANG Y B,et al.,2021.Research progress of waste PETrecycling.Plastics,50(5):61-66.]
李磊,高鑫,齐宏斌,等,2022.现代生物技术推动塑料中聚对苯二甲酸乙二酯绿色降解的研究进展.合成生物学,3(4):763-780.[LI L,GAO X,QI H B,et al.,2022.Research progress of modern biotechnology-promoted green degradation of polyethylene terephthalate in plastics.Synthetic Biology Journal,3(4):763-780.]
李清筱,2023.生物降解微塑料的机制和研究现状.食品与发酵工业,49(15):311-319.[LI Q X,2023.Mechanism and research status of biodegradable microplastics.Food and Fermentation Industries,49(15):311-319.]
李秀,杨海涛,王泽方,2019.聚对苯二甲酸乙二醇酯降解酶的研究进展.微生物学报,59 (12):2251-2262.[LI X,YANG H T,WANG Z F,2019.Advance in polyethylene terephthalate degrading enzyme.Acta Microbiologica Sinica,59(12):2251-2262.]
李旭明,师利芬,钱志华,等,2012.脂肪酶处理对涤纶织物亲水性能的改善.纺织学报,33(4):91-94.[LI X M,SHIL F,QIAN Z H,et al.,2012.Improvement of wettabi-lity of PET fabrics treated by lipase.Journal of Textile Research,33(4):91-94.]
刘欣悦,耿文超,孙瑨原,等,2024.基于结构信息的MHET降解酶挖掘及温和温度下的酶级联塑料降解.生物工程学报,40(3):773-785.[LIU X Y,GENG W C,SUN J Y,et al.,2024.Structure motif guided mining of MHET hydrolase and development of a two-enzyme cascade for plastics depolymerization at mild temperature.Chinese Journal of Biotechnology,40(3):773-785.]
娄文勇,吴有权,郭泽望,等,2022.角质酶的异源表达和超声辅助下降解PET塑料.华南理工大学学报(自然科学版),50(1):132-142.[LOU W Y,WU Y Q,GUO Z W,et al.,2022.Heterologous expression of cutinase and degradation of polyethylene terephthalate with ultrasound.Journal of South China University of Technology (Natural Science Edition),50(1):132-142.]
邱语馨,李来一,林来昌,等,2025.宏基因组揭示西藏市政污水中微生物的塑料降解功能潜力.厦门大学学报(自然科学版),64(3):538-545.[QIU Y X,LI L Y,LIN L C,et al.,2025.Metagenomics reveals the plastic degradation potential of microorganisms in municipal sewage in Xizang.Journal of Xiamen University (Natural Science),64 (3):538-545.]
魏漪,肖云杰,杨海涛,等,2023.聚对苯二甲酸乙二醇酯水解酶IsPETase及其应用展望.微生物学报,63(1):15-29.[WEI Y,XIAO Y J,YANG H T,et al.,2023.Polyethylene terephthalate hydrolase IsPETase and its application prospect.Acta Microbiologica Sinica,63(1):15-29.]
伍换,蒋小良,李轩,等,2022.食品包装高分子材料的种类及安全性.当代化工研究(17):10-12.[WU H,JIANG XL,LI X,et al.,2022.Type and safety of food packaging polymer materials.Modern Chemical Research,(17):10-12.]
杨媚媛,樊芳芳,何灵娟,等,2024.对苯二甲酸单羟乙酯水解酶结构与功能的研究进展.生物工程学报,40 (9):2812-2830.[YANG M Y,FAN F F,HE L J,et al.,2024.Advances in the structure and function of MHETase.Chinese Journal of Biotechnology,40(9):2812-2830.]
余林玲,张盼盼,孙彦,2024.有机溶剂和聚电解质提升MHET水解酶催化性能.天津大学学报(自然科学与工程技术版),57(5):452-461.[YU L L,ZHANG P P,SUNY,2024.Catalytic performance of mono (2-hydroxyethyl)terephthalate hydrolase enhanced by organic solvents and polyelectrolytes.Journal of Tianjin University (Science and Technology),57(5):452-461.]
张馨月,袁博,白晓拴,2023.PET生物降解酶的研究进展.内蒙古林业科技,49(3):59-64.[ZHANG X Y,YUAN B,BAI X S,2023.Research progress of PET biodegrading enzymes.Journal of Inner Mongolia Forestry Science and Technology,49(3):59-64.]
赵彧瑾,陈仔君,赵晶晶,等,2021.聚对苯二甲酸乙二醇酯(PET)降解酶的研究进展.微生物学杂志,41(6):90-96.[ZHAO Y J,CHEN Z J,ZHAO J J,et al.,2021.Advanced in degradation enzyme of polyethylene terephthalate (PET).Journal of Microbiology,41(6):90-96.]
ACERO E H,RIBITSCH D,STEINKELLNER G,et al.,2011.Enzymatic surface hydrolysis of PET:effect of structural diversity on kinetic properties of cutinases from Thermobifida.Macromolecules,44(12):4632-4640.
AGUIAR M I S,SOUSA A F,TEIXEIRA G,et al.,2024.Enhancing plastic waste recycling:evaluating the impact of additives on the enzymatic polymer degradation.Catal.Today,429:114492.
ARAúJO R,SILVA C,O'NEILL A,et al.,2007.Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6,6 fibers.J.Biotechnol.,128(4):849-857.
BARTH M,HONAK A,OESER T,et al.,2016.A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films.Biotechnol.J.,11(8):1082-1087.
CHAE Y,AN Y J,2018.Current research trends on plastic pollution and ecological impacts on the soil ecosystem:a review.Environ.Pollut.,240:387-395.
CHOI H M,CHO J Y,2016.Microwave-mediated rapid tailoring of PET fabric surface by using environmentally-benign,biodegradable urea-choline chloride deep eutectic solvent.Fibers.Polym.,17(6):847-856.
?OLNIK M,KNEZ ?,?KERGET M,2021.Sub-and supercritical water for chemical recycling of polyethylene terephthalate waste.Chem.Eng.Sci.,233:116389.
CUDJOE D,ACQUAH P M,2021.Environmental impact analysis of municipal solid waste incineration in African countries.Chemosphere,265:129186.
CUI Y L,CHEN Y C,LIU X Y,et al.,2021.Computational redesign of a PETase for plastic biodegradation under ambient condition by the GRAPE strategy.ACS Catal.,11 (3):1340-1350.
EBERL A,HEUMANN S,BRüCKNER T,et al.,2009.Enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules.J.Biotechnol.,143(3):207-212.
EIAMTHONG B,MEESAWAT P,WONGSATIT T,et al.,2022.Discovery and genetic code expansion of a polyethylene terephthalate (PET) hydrolase from the human saliva metagenome for the degradation and bio-functionalization of PET.Angew.Chem.Int.Ed.Engl.,61(37):e202203061.
ERICKSON E,GADO J E,AVILáN L,et al.,2022.Sourcing thermotolerant poly (ethylene terephthalate) hydrolase scaffolds from natural diversity.Nat.Commun.,13(1):7850.
FECKER T,GALAZ-DAVISON P,ENGELBERGER F,et al.,2018.Active site flexibility as a hallmark for efficient PETdegradation by I.sakaiensis PETase.Biophys.J.,114 (6):1302-1312.
GAN L L,XIAO Z H,PAN H,et al.,2021.Efficiently production of micron-sized polyethylene terephthalate (PET) powder from waste polyester fibre by physicochemical method.Adv.Powder Technol.,32(2):630-636.
GOODES L M,WONG E V S,ALEX J,et al.,2022.A scoping review protocol on in vivo human plastic exposure and health impacts.Syst.Rev.,11(1):137.
GUO W B,DUAN J J,SHI Z G,et al.,2023.Biodegradation of PET by the membrane-anchored PET esterase from the marine bacterium Rhodococcus pyridinivorans P23.Commun.Biol.,6(1):1090.
HAN X,LIU W D,HUANG J W,et al.,2017.Structural insight into catalytic mechanism of PET hydrolase.Nat.Commun.,8(1):2106.
HOSOKAWA M,2008.Structure and catalytic properties of carboxylesterase isozymes involved in metabolic activation of prodrugs.Molecules,13(2):412-431.
JOO S,CHO I J,SEO H,et al.,2018.Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation.Nat.Commun.,9(1):382.
KAWAI F,ODA M,TAMASHIRO T,et al.,2014.A novel Ca2+-activated,thermostabilized polyesterase capable of hydrolyzing polyethylene terephthalate from Saccharomonospora viridis AHK190.Appl.Microbiol.Biotechnol.,98(24):10053-10064.
KLEEBERG I,WELZEL K,VANDENHEUVEL J,et al.,2005.Characterization of a new extracellular hydrolase from Thermobifida fusca degrading aliphatic-aromatic copolyesters.Biomacromolecules,6(1):262-270.
KNOTT B C,ERICKSON E,ALLEN M D,et al.,2020.Characterization and engineering of a two-enzyme system for plastics depolymerization.Proc.Natl.Acad.Sci.USA,117(41):25476-25485.
KUMAR R,MANNA C,PADHA S,et al.,2022.Micro(nano)plastics pollution and human health:how plastics can induce carcinogenesis to humans?.Chemosphere,298:134267.
LU H Y,DIAZ D J,CZARNECKI N J,et al.,2022.Machine learning-aided engineering of hydrolases for PET depolymerization.Nature,604(7907):662-667.
MüLLER R J,SCHRADER H,PROFE J,et al.,2005.Enzymatic degradation of poly(ethylene terephthalate):rapid hydrolyse using a hydrolase from T.fusca.Macromol.Rapid Commun.,26(17):1400-1405.
PALM G J,REISKY L,B?TTCHER D,et al.,2019.Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate.Nat.Commun.,10(1):1717.
PINTO A V,FERREIRA P,NEVES R P P,et al.,2021.Reaction mechanism of MHETase,a PET degrading enzyme.ACSCatal.,11(16):10416-10428.
RIBITSCH D,HEUMANN S,TROTSCHA E,et al.,2011.Hydrolysis of polyethylene terephthalate by p-nitrobenzylesterase from Bacillus subtilis.Biotechnol.Prog.,27 (4):951-960.
RONKVIST?M,XIE W C,LU W H,et al.,2009.Cutinasecatalyzed hydrolysis of poly(ethylene terephthalate).Macromolecules,42(14):5128-5138.
SAFDAR A,ISMAIL F,IMRAN M,2024.Biodegradation of synthetic plastics by the extracellular lipase of Aspergillus niger.Environ.Adv.,17:100563.
SAGONG H Y,SEO H,KIM T,et al.,2020.Decomposition of the PET film by MHETase using exo-PETase function.ACSCatal.,10(8):4805-4812.
SILVA C,DA S,SILVA N,et al.,2011.Engineered Thermobifida fusca cutinase with increased activity on polyester substrates.Biotechnol.J.,6(10):1230-1239.
SILVA C M,CARNEIRO F,O'NEILL A,et al.,2005.Cutinase:a new tool for biomodification of synthetic fibers.J.Polym.Sci.Part A Polym.Chem.,43(11):2448-2450.
SULAIMAN S,YAMATO S,KANAYA E,et al.,2012.Isolation of a novel cutinase homolog with polyethylene terephthalatedegrading activity from leaf-branch compost by using a metagenomic approach.Appl.Environ.Microbiol.,78 (5):1556-1562.
SULAIMAN S,YOU D J,KANAYA E,et al.,2014.Crystal structure and thermodynamic and kinetic stability of metagenome-derived LC-cutinase.Biochemistry,53 (11):1858-1869.
TOURNIER V,TOPHAM C M,GILLES A,et al.,2020.An engineered PET depolymerase to break down and recycle plastic bottles.Nature,580(7802):216-219.
VILJAKAINEN V R,HUG L A,2021.New approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes.Comput.Struct.Biotechnol.J.,19:6191-6200.
WEI R,BREITE D,SONG C,et al.,2019.Biocatalytic degradation efficiency of postconsumer polyethylene terephthalate packaging determined by their polymer microstructures.Adv.Sci.,6(14):1900491.
YIN Q D,YOU S P,ZHANG J X,et al.,2022.Enhancement of the polyethylene terephthalate and mono-(2-hydroxyethyl)terephthalate degradation activity of Ideonella sakaiensis PETase by an electrostatic interaction-based strategy.Bioresour.Technol.,364:128026.
YOSHIDA S,HIRAGA K,TAKEHANA T,et al.,2016.A bacterium that degrades and assimilates poly (ethylene terephthalate).Science,351(6278):1196-1199.
ZHU B T,WANG D,WEI N,2022.Enzyme discovery and engineering for sustainable plastic recycling.Trends Biotechnol.,40(1):22-37.
基本信息:
DOI:10.13417/j.gab.044.000781
中图分类号:TQ925;X705
引用信息:
[1]董心雨,吴昊.PET塑料降解酶及其降解效率研究进展[J].基因组学与应用生物学,2025,44(08):781-790.DOI:10.13417/j.gab.044.000781.
基金信息:
湖南省科技创新计划项目(2023RC3137)资助
2025-06-27
2025-06-27
2025-06-27