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柑橘黄龙病导致柑橘属(Citrus)植物叶片出现异常的淀粉积累，其分子机制尚未明确。为解析淀粉代谢生物学过程在柑橘与黄龙病菌互作过程中的作用，本研究利用生物信息学手段鉴定甜橙(Citrus sinensis)的淀粉合成相关基因，分析甜橙淀粉合成相关基因的染色体分布、系统发生关系、基因结构和启动子区域顺式作用元件，鉴定编码蛋白的保守基序和结构域，探索甜橙淀粉合成相关基因在柑橘黄龙病菌(Candidatus Liberibacter asiaticus, CLas)和溃疡病菌(Xanthomonas citri subsp.citri, Xcc)侵染过程中的表达模式和转录调控网络。研究结果发现，甜橙中存在17个淀粉合成相关基因，不均匀地分布在8条染色体上；甜橙淀粉合成相关基因的外显子数目为9～22个；甜橙淀粉合成相关蛋白与拟南芥(Arabidopsis thaliana)淀粉合成相关蛋白聚为4簇，其编码的蛋白质具有与生物学功能一致的保守基序或结构域；甜橙淀粉合成相关基因的启动子含有多种参与生物和非生物胁迫的顺式作用元件，在柑橘黄龙病菌和溃疡病菌侵染过程中，这些基因的表达模式发生显著变化。此外，多种转录因子参与调控甜橙淀粉合成相关基因的表达。本研究为深入分析淀粉合成代谢参与甜橙应答病原菌侵染提供数据支撑，为解析植物能量代谢与免疫之间的关系提供新的见解。
Abstract：Citrus Huanglongbing leads to abnormal starch accumulation in Citrus plant leaves, and its molecular mechanisms are not yet clear. In order to elucidate the role of starch metabolism in the interaction between citrus and Candidatus Liberibacter spp., this study used bioinformatics methods to identify the genes related to starch synthesis in Citrus sinensis, analyze the chromosomal distribution, phylogenetic relationships, gene structure, and cis-acting elements in the promoter regions of genes related to sweet orange starch synthesis, identify conserved motifs and structural domains of proteins, and explore the expression pattern and transcriptional regulatory network of genes related to starch synthesis in Citrus sinensis during infection by Candidatus Liberibacter asiaticus and Xanthomonas citri subsp. citri. The results of study found that there are 17 starch synthesis-related genes in Citrus sinensis, unevenly distributed on 8 chromosomes; the number of exons in genes related to starch synthesis in C. sinensis ranges from 9 to 22; proteins related to starch synthesis in C. sinensis cluster into 4 groups with proteins related to starch synthesis in Arabidopsis thaliana; proteins encoded by starch synthesis-related genes have conserved motifs or structural domains consistent with their biological functions; the promoter regions of genes related to starch synthesis in Citrus sinensis contain various cis-acting elements involved in biotic and abiotic stresses; the expression patterns of genes related to starch synthesis in C. sinensis change significantly during the infection process by Candidatus Liberibacter asiaticus and Xanthomonas citri subsp. citri. In addition, multiple transcription factors are involved in regulating the expression of genes related to starch synthesis in Citrus sinensis. This study provides data support for in-depth analysis of starch synthesis metabolism in response to pathogen infection in C. sinensis, and offers new insights into the relationship between plant energy metabolism and immunity.
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基本信息:
DOI：10.13417/j.gab.044.000121
中图分类号:S436.66
引用信息:
[1]刘新军,曾宪章,包凯强,等.甜橙淀粉合成相关基因鉴定及应答病原菌感染的表达模式分析[J].基因组学与应用生物学,2025,44(02):121-133.DOI:10.13417/j.gab.044.000121.
基金信息:
国家自然科学基金项目(32260659)资助
2025-02-28
2025-02-28
2025-02-28