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本文运用RT-PCR方法克隆了黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV)湖南邵阳株系的基因组MP(CGMMV-HuNSY movement protein)片段,测序并进行了分析。结果显示,片段全长共有803bp(KC684977),编码由264个氨基酸组成的蛋白质,推测分子量约28.87kD,理论等电点pI为9.06,ProtParam预测显示为不稳定蛋白,与已报道的辽宁分离物病毒的MP作比较,核苷酸相似性为99.6%,氨基酸相似性为98.9%;湖南邵阳株系CGMMVMP蛋白无高度卷曲螺旋部位,有跨膜结构区域,该部位表现为疏水性,可能为蛋白互作位点;磷酸化位点均匀分布于整个多肽链中,存在5个主要的B细胞抗原表位预测位点;对烟草花叶病毒属病毒MP氨基酸序列进行了motif查找,发现了该属病毒氨基酸序列的3个保守区段,还进行了密码子偏向性分析。此外,发现了1个酰胺化位点和1个依赖于cAMP和cGMP的蛋白激酶磷酸化位点,可能与病毒的侵染机制有关。
Abstract：The genome segment of MP (movement protein) of cucumber green mottle mosaic virus of hunan Shaoyang isolate was cloned by using the RT-PCR method, sequencing and analysis. The result show that the MP had 803 nucleotides (KC684977) encoding a polypeptide of 264 amino acids with a molecular weight of 28.87 kD and an isoelectric point of 9.06, it was predicted that the protein as unstable by ProtParam. Compared with Liaoning isolates, the similarities of nucleotide and amino acid respectively are 99.6% and 98.9%. There are not highly coiled coil regions on CGMMV MP protein, but find transmembrane helices with hydrophobic and it is a potential protein interaction sites, Phosphorylation sites uniform distribution in the whole of the polypeptide chain, there are five major B cell antigen epitope prediction sites. By searching the motif of tobamo virus MP amino acids, we found that three conservative sections, and amino acids exist codon bias. Moreover, we found a amidation site and a cAMP- and cGMP-dependent protein kinase phosphorylation site, which may involved in virus infection mechanism.
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基本信息:
中图分类号:S436.421
引用信息:
[1]张亚东,赵慧茹,周倩,等.黄瓜绿斑驳花叶病毒湖南邵阳株系基因组MP片段的测定及生物信息学分析[J].基因组学与应用生物学,2013,32(03):325-331.
基金信息:
国家支撑计划项目南方丘陵山地农区农业面源污染防控技术集成与示范(2012BAD15B04-1)资助
2013-06-28
2013-06-28