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采用RACE-PCR技术,获得中华绒螯蟹胰岛素样促雄激素腺基因(Es-IAG)全长cDNA序列,并利用相关生物信息学软件对该基因及其蛋白质的结构、理化特性进行生物信息学分析。结果发现,Es-IAG基因序列全长1 392 bp,编码151个氨基酸,Es-IAG多肽的相对分子量为16.61 kD,理论等电点pI为5.08,前体多肽中存在分泌型信号肽,存在两个糖基化位点和16个磷酸化位点,存在两个典型的R**R蛋白酶酶切位点,未发现跨膜结构域。Es-IAG为分泌性蛋白,蛋白需要通过结合细胞膜上的受体而发挥性别调控等作用。对22个近缘物种的IAG基因序列系统进化分析显示,中华绒螯蟹与蓝蟹、拟穴青蟹的亲缘关系较近。为深入研究Es-IAG基因及其蛋白的结构和功能提供了相关依据。
Abstract：The full length cDNA sequence of insulin-like androgenic gland hormone gene from Eriocheir sinensis was cloned using RACE-PCR technology and the structure, physical and chemical characteristics were analyzed using relevant bioinformatics softwares. The results showed that the Es-IAG gene is 1 392 bp in length and encodes175 aa with the relative molecular weight 16.61 k D. The theoretic isoelectric point is 5.08. There are one secretional signal peptide, two glycosylation sites and 16 phosphorylation sites in the precursor peptides. Two typical R**R protease cutting sites and no transmembrane domain are found. Es-IAG is a kind of secreted protein,which needs to bind receptors on the cell membrane to play regulatory roles. The evolutionary analysis of the IAG gene sequence system of 22 proximal species shows that the Chinese mitten crab is closely related to the blue crab and the green crab. These results provide theoretical basis for further study on structure and functions of Es-IAG gene and its encoded protein.
Chung J.S.,Manor R.,and Sagi A.,2011,Cloning of an insulin-like androgenic gland factor(IAG)from the blue crab,Callinectes sapidus:implications for eyestalk regulation of IAG expression,General and Comparative Endocrinology,173(1):4-10
Fu C.,Zeng Q.,Li F.,Wang H.,Sun J.,and Wang H.,2017,Comparative transcriptome analysis reveals related regulatory mechanisms of androgenic gland in Eriocheir sinensis,Biomed Research International,(1):1-12
Li F.J.,Jiang F.W.,Bai H.K.,Fu H.T.,Jin S.B.,Sun S.M.,Qiao H.,and Zhang W.Y.,2015a,Genomic cloning,expression and single nucleotide polymorphism association analysis of the insulin-like androgenic gland hormone gene in the oriental river prawn(Macrobrachium nipponense),Genetics and Molecular Research Gmr,14(2):5910-5921
Li F.J.,Bai H.K.,Xiong Y.W.,Fu H.T.,Jiang S.F.,Jiang F.W.,Jin S.B.,Sun S.M.,Qiao H.,and Zhang W.Y.,2015b,Molecular characterization of insulin-like androgenic gland hormone-binding protein gene from the oriental river prawn Macrobrachium nipponense and investigation of its transcriptional relationship with the insulin-like androgenic gland hormone gene,General and Comparative Endocrinology,216(6):152-160
Li S.,Li F.,Sun Z.,and Xiang J.,2012,Two spliced variants of insulin-like androgenic gland hormone gene in the Chinese shrimp,Fenneropenaeus chinensis,General and Comparative Endocrinology,177(2):246-255
Manor R.,Weil S.,Oren S.,Glazer L.,Aflalo E.D.,Ventura T.,Chalifa-Caspi V.,Lapidot M.,and Sagi A.,2007,Insulin and gender:an insulin-like gene expressed exclusively in the androgenic gland of the male crayfish,General and Comparative Endocrinology,150(2):326-336
Mareddy V.R.,Rosen O.,Thaggard H.B.,Manor R.,Kuballa A.V.,Aflalo E.D.,Sagi A.,Paterson B.,and Elizur A.,2011,Isolation and characterization of the complete cDNA sequence encoding a putative insulin-like peptide from the androgenic gland of Penaeus monodon,Aquaculture,318(3-4):364-370
Nagamine C.,Knight A.W.,Maggenti A.,and Paxman G.,1980,Effects of androgenic gland ablation on male primary and secondary sexual characteristics in the malaysian prawn,Macrobrachium rosenbergii(decapoda,palaemonidae),with first evidence of induced feminization in a nonhermaphroditic decapod,General and Comparative Endocrinology,41(4):423-441
Qiu G.F.,and Wu P.,2000,Structure and function of the androgenic gland in Eriocheir sinensis,Shuichan Xuebao(Journal of Fisheries of China),24(2):108-112(邱高峰,吴萍,2000,中华绒螯蟹促雄腺的结构与功能,水产学报,24(2):108-112)
Sagi A.,and Aflalo E.D.,2005,The androgenic gland and monosex culture of freshwater prawn Macrobrachium rosenberg:a biotechnological perspective,Aquaculture Research,36(3):231-237
Sagi A.,Cohen D.,and Milner Y.,1990,Effect of androgenic gland ablation on morphotypic differentiation and sexual characteristics of male freshwater prawns,Macrobrachium rosenbergii,General and Comparative Endocrinology,77(1):15-22
Sagi A.,and Khalaila I.,2001,The crustacean androgen:a hormone in an isopod and androgenic activity in decapods,American Zoologist,41(3):477-484
Sroyraya M.,Chotwiwatthanakun C.,Stewart M.J.,Soonklang N.,Kornthong N.,Phoungpetchara I.,Hanna P.J.,and Sobhon P.,2010,Bilateral eyestalk ablation of the blue swimmer crab,Portunus pelagicus,produces hypertrophy of the androgenic gland and an increase of cells producing insulin-like androgenic gland hormone,Tissue and Cell,42(5):293-300
Ventura T.,Manor R.,Aflalo E.D.,Weil S.,Raviv S.,Glazer L.,and Sagi A.,2009,Temporal silencing of an androgenic gland-specific insulin-like gene affecting phenotypical gender differences and spermatogenesis,Endocrinology,150(3):1278-1286
Ventura T.,Manor R.,Aflalo E.D.,Weil S.,Rosen O.,and Sagi A.,2012,Timing sexual differentiation:Full functional sex reversal achieved through silencing of a single insulin-like gene in the prawn,Macrobrachium rosenbergii,Biology of Reproduction,86(3):90
Ventura T.,and Sagi A.,2012,The insulin-like androgenic gland hormone in crustaceans:From a single gene silencing to a wide array of sexual manipulation-based biotechnologies,Biotechnology Advances,30(6):1543-1550
Wang G.L.,Wei R.B.,and Qiu G.F.,2004,The effect of 17β-estradiol on the tissue structure and ultrastructure of androgenic gland of Chinese mitten crab Eriocheir sinensis,Shanghai Haiyang Daxue Xuebao(Journal of Shanghai Fisheries University),13(1):10-15(汪桂玲,韦荣编,邱高峰,2004,17β-雌二醇对中华绒螯蟹促雄腺组织结构和超微结构的影响,上海海洋大学学报,13(1):10-15)
Wu P.,Qiu G.F.,and Lou Y.D.,2002,The effects of the structure changes of the androgenic gland on the spermatogenesis in Eriocheir sinensis,Shuichanxue Zazhi(Chinese Journal of Fisheries),15(1):88-92(吴萍,邱高峰,楼允东,2002,中华绒螯蟹促雄腺结构变化对精子发生的影响,水产学杂志,15(1):88-92)
Wu Z.H.,Du J.,Tan H.L.,Tiemuer A.,Zhang P.,and Wu B.,2017,Cloning and sequence analysis of lipoxygenase PaLOXgene from small white apricot,Shengwu Jishu(Biotechnology),(1):1-8(吴忠红,杜鹃,谭慧林,阿塔吾拉·铁木尔,张平,吴斌,2017,小白杏脂氧合酶基因PaLOX的克隆与序列分析,生物技术,(1):1-8)
Xiao M.N.,Xu J.N.,Cai S.L.,and Liu H.,2010,Preliminary study on isolation and purification of the androgenic gland hormone of Eriocheir sinensis,Shanghai Haiyang Daxue Xuebao(Journal of Shanghai Fisheries University),19(1):28-33(肖美南,徐佳念,蔡生力,刘红,中华绒螯蟹促雄性腺素分离、纯化的初步研究,上海海洋大学学报,19(1):28-33)
Xiao Z.,Li J.Y.,Fan Z.Q.,Li X.L.,and Yin H.F.,2016,Cloning and expression analysis of lipohylol tea GA2ox1 gene,Linye Kexue Yanjiu(Forestry Science Research),29(1):41-47(肖政,李纪元,范正琪,李辛雷,殷恒福,荔波连蕊茶GA2ox1基因的克隆及表达分析,林业科学研究,29(1):41-47)
基本信息:
DOI：10.13417/j.gab.038.003946
中图分类号:S917.4
引用信息:
[1]付春鹏,于杰伦,李法君,等.中华绒螯蟹IAG基因的克隆及生物信息学分析[J].基因组学与应用生物学,2019,38(09):3946-3951.DOI:10.13417/j.gab.038.003946.
基金信息:
山东半岛卤水资源高值化绿色化综合利用工程技术研发中心资助项目(2018LS015; 2018LS009);; 山东省“双一流”奖补资金;; 山东省海洋与渔业科技创新计划项目(2017YY27);; 山东省研究生教育创新计划项目(SDYY11083; SDYC12048)共同资助
2019-09-25
2019-09-25