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利用CRISPR/Cas9技术靶向编辑水稻(Oryza sativa)类囊体腔蛋白基因OsTLP16.5(Os06g0705100)，使该基因编码的大部分氨基酸序列发生改变，经筛选鉴定获得突变体ostlp16.5。对水稻突变体ostlp16.5及野生型ZH11进行代谢组检测，结果表明，有机酸、氨基酸、维生素及其衍生物等物质的含量在突变体ostlp16.5和野生型ZH11中表现出显著差异。进一步分析发现，突变体ostlp16.5与野生型ZH11差异较大的代谢产物(3,4二羟基苯基丙酸、多巴胺、L-花生酸和L-酪氨酸、L-2-羟基戊二酸、2-酮基戊二酸、泛酸等)及差异显著的代谢通路(异喹啉生物碱生物合成、酪氨酸代谢等)大部分直接或间接与光合反应相关，表明OsTLP16.5蛋白可能通过参与光合调控来影响水稻体内有机酸、氨基酸和维生素等的代谢。研究结果对揭示水稻类囊体腔蛋白OsTLP16.5的功能及提高水稻的生产力具有重要意义，并可为研究OsTLP16.5蛋白调控水稻体内次生代谢及生长发育提供参考。
Abstract：Targeted editing the rice(Oryza sativa) thylakoid lumen protein gene OsTLP16.5(Os06g0705100) using CRISPR/Cas9technology,most of the amino acid sequences encoded by the gene were changed,and the mutant ostlp16.5 was screened and identified.Metabonomic analysis of rice mutant ostlp16.5 and wild type ZH11 showed that the contents of organic acids,amino acids,vitamins and their derivatives were significantly different between mutant ostlp16.5 and wild type ZH11.Further analysis showed that the metabolites(3,4-dihydroxyphenylpropanoate,dopamine,L-arogenate acid and L-tyrosine,L-2-hydroxyglutaric acid,2-oxoarginine,pantothenic acid,etc.) and the metabolic pathways(isoquinoline alkaloid biosynthesis,tyrosine metabolism,etc.) with significant differences between mutant ostlp16.5 and wild type ZH11 were mostly directly or indirectly related to photosynthetic reaction.These results indicated that OsTLP16.5 protein may affect the metabolism of organic acids,amino acids and vitamins in rice by participating in photosynthetic regulation.The results are important for revealing the function of OsTLP16.5 and improving the productivity for rice,and could provide a reference for studying OsTLP16.5 protein regulation of secondary metabolism and growth and development in rice.
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基本信息:
DOI：10.13417/j.gab.042.000359
中图分类号:S511
引用信息:
[1]王雅利,康振辉.水稻类囊体腔蛋白基因OsTLP16.5突变体的代谢组分析[J].基因组学与应用生物学,2023,42(04):359-372.DOI:10.13417/j.gab.042.000359.
基金信息:
国家自然科学基金青年基金项目(31601291); 四川省科技厅重点计划项目(2020YFN0023); 地方项目五粮液产学研合作项目(CXY2021ZR010)共同资助
2023-05-05
2023-05-05
2023-05-05