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于2015年5月至2016年3月对太湖湖滨湿地浮游细菌群落结构及时间动态进行考察。运用实时荧光定量PCR考察了浮游细菌生物量的动态变化;通过16S r RNA基因高通量测序及聚类分析研究了浮游细菌群落组成及时间动态;并运用LEf Se分析探究了浮游细菌群落的主要差异物种;通过冗余分析探究了环境因子对浮游细菌群落结构变化的驱动作用。结果显示:太湖湖滨湿地浮游细菌主要分布于12个门中的24个纲,其中变形菌门、放线菌门、拟杆菌门及蓝细菌门微生物占总体相对丰度的90.11%。浮游细菌生物量在7月达到最高(2.25×105 copies/L),同时Shannon-Winner指数及Simpson指数别为5.313±0.124与0.010±0.002,显示群落多样性亦高于其他采样时间;1月浮游细菌生物量仅次于7月(1.70×105 copies/L),而群落多样性指数较其他采样时间显著下降,这与Flavobacteria纲微生物相对丰度大幅上升有关。较高的DO值与较低的温度及NH3-N含量是1月水体Flavobacteria纲微生物快速繁殖的重要原因。
Abstract：The community structure and temporal dynamics of bacterioplankton in lakeside wetland of Tai Lake from May 2015 to June 2016 were investigated. Real-time quantitative PCR was applied to monitor the dynamic changes of planktonic bacteria biomass. 16 S r RNA high throughput sequencing and cluster analysis were used to study the community structure and temporal dynamics of bacterioplankton. LEfSe analysis was applied to explore the main different species of bacterioplankton community. Redundancy analysis was used to discuss the driving effects of environmental factors on the change of bacterioplankton community. The results showed that bacterioplankton in lakeside wetland of Tai Lake mainly distributed in 24 classes belonging to 12 phyla, of which Proteobacteria, Actinobacteria, Bacteroidetes and Cyanobacteria covered 90.11% of total relative abundance. The biomass of bacterioplankton peaked in July(2.25×105 copies/L). Meanwhile, Shannon-Winner index and Simpson index were 5.313±0.124 and 0.010±0.002, respectively, indicating the community diversity was also higher than that of other sampling times. The biomass of bacterioplankton in January was 1.70 ×105 copies/L, only second to that of July, while the community diversity index decreased significantly compared with other sampling times,which was related to the significant increase in the relative abundance of Flavobacteria microbes. The relative high DO, low temperature and low NH3-N content in January were the important reasons for the rapid growth of Flavobacteria microbes.
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基本信息:
DOI：10.13417/j.gab.037.005325
中图分类号:Q938.8
引用信息:
[1]钱玮,张济凡,张铭连,等.太湖湖滨湿地浮游细菌群落结构及时间动态[J].基因组学与应用生物学,2018,37(12):5325-5331.DOI:10.13417/j.gab.037.005325.
基金信息:
苏州市科技计划项目“基于微生物多样性的苏州湿地健康评价研究”(SS201616)和“不同施肥条件对苏州茶园土壤微生物多样性的影响研究”(SYN201414)共同资助
2018-03-06
2018-03-06
2018-03-06