| 404 | 1 | 110 |
| 下载次数 | 被引频次 | 阅读次数 |
芸薹属(Brassica)植物(甘蓝型油菜,白菜,甘蓝)是重要的经济作物,它们之间的关系可用禹氏三角表示。CAMTA是在进化过程中高度保守的一类转录因子家族,该家族成员在芸薹属植物抗逆境胁迫过程中具有重要作用。为了探讨芸薹属CAMTA3基因家族的功能,本研究利用生物信息学手段,鉴定了芸薹属(白菜,甘蓝和甘蓝型油菜) CAMTA3基因家族的8个基因,并从蛋白质的理化性质、家族进化与基因结构和上游启动子等方面进行了分析研究。分析表明,芸薹属CAMTA3基因家族8个成员在进化过程中具有较高的保守性,其亲缘关系与禹氏三角理论相符,且各成员在植物响应抗胁迫过程中占重要地位。研究结果为芸薹属CAMTA3基因家族的功能研究提供了一定的信息基础。
Abstract：B. oleracea, B. napus and B. rapa are important economic crops, belonging Brassica, and the relationship between them can be expressed in the theory of Yu's triangle. CAMTA is a family of transcription factors that are highly conserved during evolution. This family member plays an important role in the stress resistance of Brassica plants. In order to investigate the function of the CAMTA3 gene family of Brassica, this study used bioinformatics to identify eight genes of the CAMTA3 gene family of Brassica(B. napus, B. rapa, B. oleracea) and evolved from the physicochemical properties and family of proteins. Analytical studies were carried out with regard to gene structure and upstream promoters. The analysis showed that the members of the eight CAMTA3 gene families were highly conserved during the evolution process, and their genetic relationship was consistent with the theory of Yu's triangle. At the same, each member played an important role in plant response to stress. The results provided a certain information basis for the functional study of the CAMTA3 gene family in Brassica.
Doherty C.J.,Van Buskirk H.A.,Myers S.J.,and Thomashow M.F.,2009,Roles for Arabidopsis CAMTA transcription factors in cold-regulated gene expression and freezing tolerance,Plant Cell,21(3):972-984.
Du L.Q.,Ali G.S.,Simons K.A.,Hou J.G.,Yang T.B.,Reddy A.S.N.,and Poovaiah B.W.,2009,Ca2+/calmodulin regulates salicylic-acid-mediated plant immunity,Nature,457(7233):1154-1158.
Galon Y.,Nave R.,Boyce J.M.,Nachmias D.,Knight M.R.,and Fromm H.,2008,Calmodulin-binding transcription activator (CAMTA) 3 mediates biotic defense responses in Arabidopsis,FEBS Lett.,582(6):943-948.
Gao H.H.,Zhang Y.X.,Hu S.W.,and Guo Y.,2017,Genome-wide survey and phylogenetic analysis of MADS-box gene family in Brassica napus,Zhiwu Xuebao (Bulletin of Botany),52(6):699-712.(高虎虎,张云霄,胡胜武,郭媛,2017,甘蓝型油菜MADS-box基因家族的鉴定与系统进化分析,植物学报,52(6):699-712.)
Harberd D.J.,1972,A contribution to the cyto-taxonomy of Brassica (Cruciferae) and its allies,Bot.J.Linn.Soc.,65(1):1-23.
Kakar K.U.,Nawaz Z.,Cui Z.Q.,Cao P.J.,Jin J.J.,Shu Q.Y.,and Ren X.L.,2018,Evolutionary and expression analysis of CAMTA gene family in Nicotiana tabacum yielded insights into their origin,expansion and stress responses,Sci.Rep.,8(1):10322.
Kim Y.S.,Park S.,Gilmour S.J.,and Thomashow M.F.,2013,Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis,Plant J.,75(3):364-376.
Li X.H.,Huang L.,Zhang Y.F.,Ouyang Z.G.,Hong Y.B.,Zhang H.J.,Li D.Y.,and Song F.M.,2014,Tomato SR/CAMTAtranscription factors Sl SR1 and Sl SR3L negatively regulate disease resistance response and Sl SR1L positively modulates drought stress tolerance,BMC Plant Biol.,14:286.
Li Z.Y.,Li M.T.,Huang R.G.,Wu X.M.,and Song Y.C.,2002,Genomic in situ hybridization (GISH) discriminates the AA,BB and CC genomes in Brassica allotteraploid species,Zhongguo Youliao Zuowu Xuebao (Chinese Journal of Oil Crop Sciences),24(1):10-14.(李宗芸,栗茂腾,黄荣桂,伍晓明,宋运淳,2002,基因组原位杂交辨别芸薹属异源四倍体AA、BB、CC基因组研究,中国油料作物学报,24(1):10-14.)
Lu J.X.,Bai H.Y.,and Zhang T.,2017,Molecular evolution of MYB28 gene family in Brassica species (B.napus,B.rapa,B.oleracea,B.juncea,B.nigra),Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(2):393-403.(陆俊杏,白辉扬,张涛,2017,芸薹属物种(B.napus,B.rapa,B.oleracea,B.juncea,B.nigra) MYB28家族的分子进化,分子植物育种,15(2):393-403.)
Lu J.X.,Lu K.,Zhu B.,Peng Q.,Lu Q.F.,Qu C.M.,Yin J.M.,Li J.N.,Liang Y.,and Chai Y.R.,2013,Cloning,evolution and expression features of MAPK1 gene family from Brassica species (B.napus,B.oleracea,B.rapa),Zhongguo Nongye Kexue (Scientia Agricultura Sinica),46(16):3478-3487.(陆俊杏,卢坤,朱斌,彭茜,陆奇丰,曲存民,殷家明,李加纳,梁颖,柴友荣,2013,芸薹属物种(B.napus,B.oleracea,B.rapa) MAPK1家族的克隆、进化和表达特征,中国农业科学,46(16):3478-3487.)
Nagaharu U.,1935,Genome analysis in Brassica with special reference to the experimental formation of B.napus and peculiar mode of fertilization,Jap.J.Bot.,7:389-452.
Reddy A.S.N.,Ali G.S.,Celesnik H.,and Day I.S.,2011,Coping with stresses:roles of calcium-and calcium/calmodulin-regulated gene expression,Plant Cell,23(6):2010-2032.
Sun F.,Xia X.L.,and Yin W.L.,2009,The mutual regulations between ABA and calcium signal transduction pathways under abiotia stress,Jiyinzuxue yu Yingyong Shengwuxue (Genomics and Applied Biology),28(2):391-397.(孙芳,夏新莉,尹伟伦,2009,逆境胁迫下ABA与钙信号转导途径之间的相互调控机制,基因组学与应用生物学,28(2):391-397.)
Wang J.,and Meng J.L.,2010,Progress on genome research of Brassica crops and its significance for breeding,Fenzi Zhiwu Yuzhong (Molecular Plant Breeding),8(5):837-845.(王晶,孟金陵,2010,芸薹属作物基因组研究进展及其在育种中的意义,分子植物育种,8(5):837-845.)
Zhou C.,Fu D.H.,and Zhou Q.H.,2014,Advance in genome evolution and comparative genetic research of Brassica species,Jiyinzuxue yu Yingyong Shengwuxue (Genomics and Applied Biology),33(5):1139-1146.(周灿,付东辉,周庆红,2014,芸薹属物种基因组进化及比较基因组学研究进展,基因组学与应用生物学,33(5):1139-1146.)
基本信息:
DOI：10.13417/j.gab.040.001238
中图分类号:Q943.2
引用信息:
[1]罗庆萱,向奥玲,张涛,等.芸薹属(Brassica napus,B.rapa,B.oleracea)CAMTA3基因家族的鉴定及生物信息学分析[J].基因组学与应用生物学,2021,40(03):1238-1247.DOI:10.13417/j.gab.040.001238.
基金信息:
重庆市科学技术局基金项目(cstc2017jcyjAX0108; cstc2019jscx-msxm0612); 重庆高校创新团队(CXTDX2016-01018); 重庆师范大学基金项目(16XYY27; 16XYY28)共同资助
2021-03-25
2021-03-25